US Patent Application for METHODS FOR SINGLE CELL NANOPORE SEQUENCING TECHNOLOGY AND DATA ANALYSIS Patent Application (Application #20240221868 issued July 4, 2024) (2024)

CROSS-REFERENCE TO RELATED PATENT APPLICATIONS

This application claims priority to and the benefit of U.S. Provisional Patent Application Ser. No. 63/430,727, filed on Dec. 7, 2022, and U.S. Provisional Patent Application Ser. No. 63/469,901, filed on May 31, 2023, all of which are incorporated herein in their entireties by reference.

STATEMENT AS TO RIGHTS UNDER FEDERALLY-SPONSORED RESEARCH

This invention was made with government support under grant number HL 160552 awarded by the National Institutes of Health. The government has certain rights in the invention.

STATEMENT REGARDING THE SEQUENCE LISTINGS

The Sequence Listings associated with this application are provided in xml format in lieu of a paper copy and are hereby incorporated by reference into the specification. The name of the xml file containing the Sequence Listings is 0116936.271US2.xml. The xml file is about 10 KB, was created on Mar. 19, 2024.

FIELD OF THE INVENTION

The present disclosure relates generally to field of bioinformatics, and more particularly to a tool to deconvolute barcoded long reads into single cell and single molecules without short reads curation nor whitelists of barcodes, and calculate both phenotypes and genotypes of the same cells for thousands of cells in parallel.

BACKGROUND OF THE INVENTION

The background description provided herein is for the purpose of generally presenting the context of the present invention. The subject matter discussed in the background of the invention section should not be assumed to be prior art merely as a result of its mention in the background of the invention section. Similarly, a problem mentioned in the background of the invention section or associated with the subject matter of the background of the invention section should not be assumed to have been previously recognized in the prior art. The subject matter in the background of the invention section merely represents different approaches, which in and of themselves may also be inventions.

Human tissues including tumors represent complex ecological systems of diverse cell types with dynamic genetic evolution and phenotypic remodeling. However, there is a lack of robust methods for tracking both genotypes (e.g., mutations) and phenotypes (e.g., gene expressions, isoforms) of individual cells to precisely trace the cellular and molecular dynamics during tissue development, particularly during tumor evolution and treatment response.

Recently, long-read single cell Nanopore sequencing of full-length RNAs (scNanoRNAseq) is emerging as a powerful technology to simultaneously profile phenotypes and genotypes of same cells, which however is challenged by lacking robust analysis and computational tools and requiring parallel short reads to curate sequencing errors.

In particular, scNanoRNAseq is transforming single cell multi-omics analysis through direct measurement of nucleotide sequences of whole gene bodies without algorithmic reconstructions. Nowadays, high throughput long-read single cell sequencing methods take advantage of droplet barcoding systems (commonly, 10× Genomics Chromium system) to barcode full-length cDNAs of single cells and sequence them on ultra-high yield third-generation sequencing (TGS) platforms. Of note, the Oxford Nanopore Technology (ONT) platform, PromethION can yield ˜100 million reads per flow cell, providing adequate coverages of thousands of single cell transcriptomes. The PacBio system, Sequel II system can yield ˜8 million high fidelity reads, which can measure hundreds of single cell transcriptomes.

However, as discussed above, the broad applications of this powerful technology are computationally challenged due to the complexity of calculating same-cell multi-omics from these long-read data. Moreover, due to higher error rates in cell barcodes (CBs) and unique molecule identifiers (UMIs) of TGS data comparing to next-generation sequencing (NGS), current methods rely on generating paralleled NGS short-read data to guide the deconvolution of CBs and UMIs, which can drastically increase experimental costs and computational complexity and often results in partial usage of data. Recently, two methods called Sockeye and BLAZE were released to detect CBs without usages of paralleled NGS data. However, both methods relied on theoretical barcode whitelist (10× Genomics states there are ˜3.6 million unique sequences for 3′ GEX with v3 chemistry). The whitelist dependence makes both methods compromised because the manufactured barcodes may deviate from theoretical random combinations, particularly, the pool size reaches to millions of molecules that are 2 edit-distance apart. Moreover, different versions of protocols may have different whitelists and misusages of wrong whitelists are not easy to tell due to their similarities. Therefore, a robust computational tool for analyzing high throughput single cell long-read data is still missing.

Therefore, there remains an imperative need for analysis and computational tools to deconvolute barcoded long reads into single cell and single molecules without short reads curation nor barcode whitelist guidance, and calculate both phenotypes and genotypes of the same cells.

SUMMARY OF THE INVENTION

In light of the foregoing, this invention discloses an analysis and computational tool, called single cell Nanopore sequencing analysis of Genotype-Phenotype simultaneously (scNanoGPS), to deconvolute barcoded long reads into single cells and single molecules without short reads curation nor the guidance of barcode whitelist, and calculate both phenotypes (gene expression, isoform) and genotypes (mutations) of same cells.

In one aspect of the invention, a method of performing single cell nanopore sequencing of genotype-phenotype simultaneously (scNanoGPS). The method comprises obtaining barcoded full-length cDNAs sequencing information of single cells; scanning the barcoded full-length cDNAs sequencing information to acquire barcoded information; curating errors in the barcode information to produce curated barcoded information; producing at least one BAM file based on the curated barcoded information; and calculating multi-omics information of the single cells based on the at least one BAM file.

In one embodiment, the barcoded full-length cDNAs sequencing information is obtained via long-read single cell nanopore sequencing technology.

In one embodiment, the method further comprises producing a FASTQ file based on the barcoded information acquired in step (2).

In one embodiment, the method further comprises refining the barcoded information acquired in step (2) before step (3) to produce refined barcoded information to detect true CBs.

In one embodiment, the method further comprises obtaining Unique Molecular Identifiers (UMIs) from the refined barcoded information; and curating at least one error in the Unique Molecular Identifiers (UMIs) in step (3) for transcriptome analysis.

In one embodiment, the method further comprises obtaining transcripts from the refined barcoded information; and curating at least one error in the transcripts in step (3) for the transcriptome analysis.

In one embodiment, the single cells multi-omics information includes at least one of gene expression matrix, isoforms profile and mutations profile.

In one embodiment, the single cells gene expression matrix is generated via: (a) calculating a UMI counts of genes in the single cells using the at least one BAM files; wherein each of the at least one BAM files is individually mapped; (b) selecting consensus reads that mapped to mature mRNA references to detect transcriptional isoforms of the single cells; and (c) calculating single cell mutation profiles from the consensus reads of the single cells.

In one embodiment, the method obviates using short read sequencing information of the single cells nor whitelist of barcode sequences as guidance for processing the barcoded full-length cDNAs sequencing information.

In one embodiment, the single cells include approximately 3000 cells per run of the long-read single cell nanopore sequencing.

In another aspect of the invention, a non-transitory computer readable medium storing a program causing a computer to execute a process of performing single cell nanopore sequencing of genotype-phenotype simultaneously (scNanoGPS), the process comprising obtaining barcoded full-length cDNAs sequencing information of single cells; scanning the barcoded full-length cDNAs sequencing information to acquire barcoded information; curating errors in the barcode information to produce curated barcoded information; producing at least one BAM file based on the curated barcoded information; and calculating multi-omics information of the single cells based on the at least one BAM file.

These and other aspects of the present invention will become apparent from the following description of the preferred embodiment taken in conjunction with the following drawings, although variations and modifications therein may be affected without departing from the spirit and scope of the novel concepts of the disclosure.

BRIEF DESCRIPTION OF THE DRAWINGS

Illustrative embodiments of the invention will hereafter be described with reference to the accompanying drawings, wherein like numerals denote like elements.

FIG. 1 shows a schematic diagram of experimental workflow and library structure of scNanoRNAseq.

FIG. 2 shows a schematic diagram of computational workflow of scNanoGPS.

FIG. 3 shows illustration of scNanoGPS methods, blue boxes represent execution items, while others indicate files.

FIG. 4 shows length distribution of raw reads of two cancer cell lines during scNanoRNAseq data processing by scNanoGPS.

FIG. 5 shows intersection of true cell barcodes detected by scNanoGPS and standard NGS approaches.

FIG. 6 shows pair-wised scatter plots of UMI counts per cell detected by two approaches (Pearson correlation P-value <2.2e-16) during scNanoRNAseq data processing by scNanoGPS.

FIG. 7 shows pair-wised scatter plots of gene detection versus UMI counts of single cells.

FIG. 8 shows single cell gene expression levels calculated by scNanoGPS and standard NGS approaches. (Pearson correlation P-value <2.2e-16.)

FIG. 9 shows heatmap of genes with significantly different expression levels in scNanoGPS and standard NGS approaches.

FIG. 10 shows density plots of length of genes with significantly different expression levels in scNanoGPS and standard NGS approaches.

FIG. 11 shows heatmap of single cell copy number profiles of H2030 calculated by CopyKAT from matched single cell transcriptomes of two approaches.

FIG. 12 shows size distributions of cDNAs, namely, the TapeStation traces of full-length cDNAs of A375 (upper panel) and H2030 (lower panel) before making sequencing libraries.

FIG. 13 shows identification of low-quality cells, namely, UMAPs of low-quality cells detected by NGS and scNanoGPS in A375 (upper panel), and UMAPs of low-quality cells detected by NGS and scNanoGPS in H2030 (lower panel).

FIG. 14 shows saturation analysis of scNanoRNAseq depths in A375 and H2030 cell lines. Pearson's correlation of single cell transcriptome profiles with ground truth (NGS 3′scRNAseq consensus profile) of A375 (upper left panel) and H2030 (upper right panel). Pearson's correlation of mini-bulk (averages of single cells within binned read depths) long read transcriptomes with ground truth of A375 (lower left panel) and H2030 (lower right panel).

FIG. 15 shows performance of scNanoGPS in dissecting cell types in the tumor microenvironment. The upper panel shows UMAP projection of major cell types of 4 frozen tumors using data processed by scNanoGPS; the middle panel shows UMAP projection of major cell types of 4 frozen tumors using NGS data; the lower panel shows concordance of cell typing results between scNanoGPS and NGS approaches. Only cells detected in both scNanoGPS and standard NGS approaches were used for calculation.

FIG. 16 shows classification of tumor and normal cells in tumor samples. The left panel shows heatmaps of single cell copy number profiles inferred from scNanoRNAseq data of four frozen tumors, and the right panel shows UMAPs of gene expression levels of known cancer markers, e.g., EGFR in RCCs and MET in melanomas.

FIG. 17 shows numbers of genes with DCIs in 7 cell types of a kidney tumor, when scNanoGPS is applied for profiling cell-type-specific isoforms in the tumor microenvironment.

FIG. 18 shows stratification of genes with DCIs (different combination of isoforms) based on status of gene expression levels and most dominant transcripts (MDTs). dMDT, distinct among cell types; sMDT, shared across cell types.

FIG. 19 shows heatmap of the cellular frequencies of top cell-type-specific MDTs.

FIG. 20 shows numbers of exons of tumor and normal cell preferred isoforms.

FIG. 21 shows pair-wised scatter plot of the numbers of exons of expressed genes in tumor and normal cells.

FIG. 22 shows top gene ontologies (MSigDB: GO: BP) of cell-type-specific DCI genes.

FIG. 23 shows examples of cellular frequencies of isoforms of genes expressing different MDTs in different cell types.

As used in the disclosure, the terms “bind” and “bound” are used in accordance with their plain and ordinary meanings and refer to an association between atoms or molecules. The association can be direct or indirect. For example, bound atoms or molecules may be directly bound to one another, e.g., by a covalent bond or non-covalent bond (e.g. electrostatic interactions (e.g. ionic bond, hydrogen bond, halogen bond), van der Waals interactions (e.g. dipole-dipole, dipole-induced dipole, London dispersion), ring stacking (pi effects), hydrophobic interactions and the like). As a further example, two molecules may be bound indirectly to one another by way of direct binding to one or more intermediate molecules, thereby forming a complex.

As used in the disclosure, the term “extension” or “elongation” is used in accordance with its plain and ordinary meanings and refer to synthesis by a polymerase of a new polynucleotide strand complementary to a template strand by adding free nucleotides (e.g., dNTPs) from a reaction mixture that are complementary to the template in the 5′-to-3′ direction. Extension includes condensing the 5′-phosphate group of the dNTPs with the 3′-hydroxy group at the end of the nascent (elongating) DNA strand.

As used in the disclosure, the term “hybridization pad” refers to one or both of two regions on either end of an interposing oligonucleotide barcode that are capable of hybridizing to single-stranded template nucleic acids. In embodiments, hybridization pads are a complement to the original target nucleic acid. In embodiments, each hybridization pad is composed of about 3 to about 40 random nucleotides (e.g. NNNNN, wherein N represents A, T, C, G nucleotides). In embodiments, each hybridization pad is composed of about 3 to about 5 random nucleotides. In embodiments, the first hybridization pad includes about 3 to about 5 nucleotides (e.g., random nucleotides) and the second hybridization pad includes about 3 to 25 nucleotides (e.g., random nucleotides). In embodiments, the first hybridization pad includes about 5 to about 15 nucleotides (e.g., random nucleotides) and the second hybridization pad includes about 5 to 15 nucleotides (e.g., random nucleotides). In embodiments, the first hybridization pad includes about 10 to about 15 nucleotides (e.g., random nucleotides) and the second hybridization pad includes about 10 to 15 nucleotides (e.g., random nucleotides). In embodiments, the hybridization pad includes a targeted primer sequence, or a portion thereof. A “targeted primer sequence” refers to a nucleic acid sequence that is complementary to a known nucleic acid region (e.g., complementary to a universally conserved region, or complementary sequences to target specific genes or mutations that have relevancy to a particular cancer phenotype). The hybridization pads may include sequences designed through computational software, e.g., Primer BLAST, LaserGene (DNAStar), Oligo (National Biosciences, Inc.), Mac Vector (Kodak/IBI) or the GCG suite of programs to optimize desired properties. In embodiments, the hybridization pad includes a limited-diversity sequence. A “limited-diversity sequence” refers to a nucleic acid sequence that includes random nucleotide regions and fixed nucleotide regions (e.g., NNANN, ANNTN, TNCNA, etc., wherein N represents random nucleotides and A, T, C, G represent fixed nucleotides). In embodiments, each hybridization pad is composed of 3 random nucleotides and 1 to 2 non-random nucleotides. In embodiments, each hybridization pad is composed of 4 random nucleotides and 1 to 2 non-random nucleotides.

As used in the disclosure, the term “barcode sequence” (which may be referred to as a “tag,” a “molecular barcode,” a “molecular identifier,” an “identifier sequence,” or a “unique molecular identifier”) refers to any material (e.g., a nucleotide sequence, a nucleic acid molecule feature) that is capable of distinguishing an individual molecule in a large heterogeneous population of molecules. Generally, a barcode sequence is unique in a pool of barcode sequences that differ from one another in sequence, or is uniquely associated with a particular sample polynucleotide in a pool of sample polynucleotides. In embodiments, the barcode sequence is a nucleotide sequence that forms a portion of a larger polynucleotide, such as an “interposing oligonucleotide barcode” (also referred to herein as an “interposing barcode” or an “oligonucleotide barcode”). In embodiments, every barcode sequence in a pool of interposing oligonucleotide barcodes is unique, such that sequencing reads comprising the barcode sequence can be identified as originating from a single sample polynucleotide molecule on the basis of the barcode sequence alone. In other embodiments, individual barcode sequences may be used more than once, but interposing oligonucleotide barcodes comprising the duplicate barcode sequences hybridize to different sample polynucleotides and/or in different arrangements of neighboring interposing oligonucleotide barcodes, such that sequence reads may still be uniquely distinguished as originating from a single sample polynucleotide molecule on the basis of a barcode sequence and adjacent sequence information (e.g., sample polynucleotide sequence, and/or one or more adjacent barcode sequences). In embodiments, barcode sequences are about or at least about 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 75 or more nucleotides in length. In embodiments, barcode sequences are shorter than 20, 15, 10, 9, 8, 7, 6, or 5 nucleotides in length. In embodiments, barcode sequences are about 10 to about 50 nucleotides in length, such as about 15 to about 40 or about 20 to about 30 nucleotides in length. In a pool of different barcode sequences, barcode sequences may have the same or different lengths. In general, barcode sequences are of sufficient length and include sequences that are sufficiently different to allow the identification of sequencing reads that originate from the same sample polynucleotide molecule. In embodiments, each barcode sequence in a plurality of barcode sequences differs from every other barcode sequence in the plurality by at least three nucleotide positions, such as at least 3, 4, 5, 6, 7, 8, 9, 10, or more nucleotide positions. In some embodiments, substantially degenerate barcode sequences may be known as random. In some embodiments, a barcode sequence may include a nucleic acid sequence from within a pool of known sequences. In some embodiments, the barcode sequences may be pre-defined.

As used in the disclosure, the term “random” in the context of a nucleic acid sequence or barcode sequence refers to a sequence where one or more nucleotides has an equal probability of being present. In embodiments, one or more nucleotides is selected at random from a set of two or more different nucleotides at one or more positions, with each of the different nucleotides selected at one or more positions represented in a pool of oligonucleotides including the random sequence. For example, a random sequence may be represented by a sequence composed of N's, where N can be any nucleotide (e.g., A, T, C, or G). For example, a four base random sequence may have the sequence NNNN, where the Ns can independently be any nucleotide (e.g., AATC). IBCs that contain a random sequence, collectively, have sequences composed of Ns within the hybridization pads, stem region, or loop region. Further, the IBCs have barcode sequences that may contain random sequence. In embodiments, a pool of IBCs may be represented by a fully random sequence, with the caveat that certain sequences have been excluded (e.g., runs of three or more nucleotides of the same type, such as “AAA” or “GGG”). In embodiments, nucleotide positions that are allowed to vary (e.g., by two, three, or four nucleotides) may be separated by one or more fixed positions (e.g., as in “NGN”).

As used in the disclosure, the terms “sequencing”, “sequence determination”, and “determining a nucleotide sequence”, are used in accordance with their ordinary meaning in the art, and refer to determination of partial as well as full sequence information of the polynucleotide being sequenced, and particular physical processes for generating such sequence information. That is, the term includes sequence comparisons, fingerprinting, and like levels of information about a target polynucleotide, as well as the express identification and ordering of nucleotides in a target polynucleotide. The term also includes the determination of the identification, ordering, and locations of one, two, or three of the four types of nucleotides within a target polynucleotide. Sequencing methods, such as those outlined in U.S. Pat. No. 5,302,509 can be carried out using the nucleotides described herein. The sequencing methods are preferably carried out with the target polynucleotide arrayed on a solid substrate. Multiple target polynucleotides can be immobilized on the solid support through linker molecules, or can be attached to particles, e.g., microspheres, which can also be attached to a solid substrate. In embodiments, the solid substrate is in the form of a chip, a bead, a well, a capillary tube, a slide, a wafer, a filter, a fiber, a porous media, or a column. In embodiments, the solid substrate is gold, quartz, silica, plastic, glass, diamond, silver, metal, or polypropylene. In embodiments, the solid substrate is porous.

Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly indicates otherwise, between the upper and lower limit of that range, and any other stated or unstated intervening value in, or smaller range of values within, that stated range is encompassed within the invention. The upper and lower limits of any such smaller range (within a more broadly recited range) may independently be included in the smaller ranges, or as particular values themselves, and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.

As used in the disclosure, the term “sequencing read” is used in accordance with its plain and ordinary meaning and refers to an inferred sequence of base pairs (or base pair probabilities) corresponding to all or part of a single DNA fragment. Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. Typical sequencers produce read lengths in the range of 100-500 bp. A sequencing read may include 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 250, or more nucleotide bases. Read length is a factor which can affect the results of biological studies. For example, longer read lengths improve the resolution of de novo genome assembly and detection of structural variants.

As used in the disclosure, the term “sequencing cycle” is used in accordance with its plain and ordinary meaning and refers to incorporating one or more nucleotides (e.g., nucleotide analogues) to the 3′ end of a polynucleotide with a polymerase, and detecting one or more labels that identify the one or more nucleotides incorporated. The sequencing may be accomplished by, for example, sequencing by synthesis, pyrosequencing, and the like. In embodiments, a sequencing cycle includes extending a complementary polynucleotide by incorporating a first nucleotide using a polymerase, wherein the polynucleotide is hybridized to a template nucleic acid, detecting the first nucleotide, and identifying the first nucleotide. In embodiments, to begin a sequencing cycle, one or more differently labeled nucleotides and a DNA polymerase can be introduced. Following nucleotide addition, signals produced (e.g., via excitation and emission of a detectable label) can be detected to determine the identity of the incorporated nucleotide (based on the labels on the nucleotides). Reagents can then be added to remove the 3′ reversible terminator and to remove labels from each incorporated base. Reagents, enzymes and other substances can be removed between steps by washing. Cycles may include repeating these steps, and the sequence of each cluster is read over the multiple repetitions.

As used in the disclosure the term “determine” can be used to refer to the act of ascertaining, establishing or estimating. A determination can be probabilistic. For example, a determination can have an apparent likelihood of at least 50%, 75%, 90%, 95%, 98%, 99%, 99.9% or higher. In some cases, a determination can have an apparent likelihood of 100%. An exemplary determination is a maximum likelihood analysis or report. As used in the disclosure, the term “identify,” when used in reference to a thing, can be used to refer to recognition of the thing, distinction of the thing from at least one other thing or categorization of the thing with at least one other thing. The recognition, distinction or categorization can be probabilistic. For example, a thing can be identified with an apparent likelihood of at least 50%, 75%, 90%, 95%, 98%, 99%, 99.9% or higher. A thing can be identified based on a result of a maximum likelihood analysis. In some cases, a thing can be identified with an apparent likelihood of 100%.

As used in the disclosure, term “gene” refers to a polynucleotide that is capable of conferring biological function after being transcribed and/or translated.

Present invention describes herein an analysis and computational tool, single cell nanopore sequencing analysis of Genotype-Phenotype simultaneously (scNanoGPS), to deconvolute barcoded long reads into single cells and single molecules without short reads curation and calculate both phenotypes (gene expression, isoform) and genotypes (mutation, fusion) of same cells.

In one embodiment, the present invention discloses scNanoGPS achieves independent deconvolution of raw data without the guidance of short-reads nor barcode whitelist and calculates genotypes-phenotypes of thousands of individual cells, addressing the major computational challenges of an emerging scNanoRNAseq technology.

In particular, the present invention performs completely independent deconvolution of error-prone long-reads into single-cells and single-molecules and calculate both genotypes and phenotypes in individual cells from scNanoRNAseq data. In concert with this tool, the present invention has removed all NGS sequencing steps and increased throughput to 3,000-6,000 transcriptomes per flow cell (PromethION). The present invention has demonstrated the accuracy and robustness of scNanoGPS and identified cell-type-specific isoforms and mutations in addition to gene expression profiles, enabling synchronous cell-lineage (genotype) and cell-fate (phenotype) tracing in human tissues.

In one embodiment, the present invention is applied scNanoGPS onto 23,587 long-read transcriptomes from 4 tumors and 2 cell-lines. Standalone, scNanoGPS deconvoluted error-prone long-reads into single-cells and single-molecules, and simultaneously accessed both phenotypes and genotypes of individual cells. The analyses of the present invention has revealed that tumor and stroma/immune cells expressed distinct combinations of isoforms (DCIs). In a kidney tumor, the present invention has identified 924 DCI genes involved in cell-type-specific functions such as PDE10A in tumor cells and CCL3 in lymphocytes. Moreover, transcriptome-wide mutation analyses identified many cell-type-specific mutations including VEGFA mutations in tumor cells and HLA-A mutations in immune cells, highlighting critical roles of different populations in tumors. Together, scNanoGPS facilitates applications of single-cell long-read sequencing technologies.

In one embodiment, scNanoGPS of the present invention performs independent barcode learning and assignment without additional NGS data, and calculate both genotypes (mutations, fusions) and phenotypes (gene expressions, isoforms) simultaneously from same cells using single cell nanopore sequencing data. scNanoGPS enables the construction of a unified cell lineage and cell fate roadmap of tumor cells to precisely delineate tumor evolution and therapeutic response.

Computational Workflow of scNanoGPS in Analyzing scNanoRNAseq Data

As shown in FIG. 1, after about 3,000-6,000 cells or nuclei are prepared, the high throughput scNanoRNAseq data are generated through 2 major steps: i) barcoding full-length cDNAs of single cells/nuclei using high throughput droplet barcoding system (10× Genomics), as steps 103 and 105 in FIG. 1; ii) performing high throughput long-read sequencing using PromethION (Oxford Nanopore Technologies), as steps 105 of FIG. 1. The sequencing data are then being processed by a computational workflow in step 107.

Accordingly, as shown in FIGS. 2-3, the computational workflow of scNanoGPS begins with quality control and scanning reads that have expected patterns of adaptor sequences, i.e., TruSeq RI adaptor on 5′-ends and TSO adaptor on 3′-ends of raw reads. Next, the present invention discloses an algorithm called integrated Crude Anchoring and Refinery Local Optimization (iCARLO) to detect true cell barcodes (CBs). In this algorithm, a raw list of CBs is determined by searching for a crude anchoring point through thresholding partial derivatives of the supporting reads of individual CBs. The threshold is then extended from crude anchoring point by an empirical percentage (˜10%) to rescue true CBs that have much less reads than others in the same experiment. Next, the CBs within two Levenshtein Distances (LDs) are curated and merged to rescue mis-assigned reads due to errors in CB sequences. CBs with less than 300 genes are filtered out by default. iCARLO is implemented in the “Assigner” function, which outputs a list of true CBs and then deconvolutes all qualified reads into single cell FASTQ files.

In one embodiment, to identify true UMIs to accurately measure gene expression levels, scNanoGPS first maps single cell reads against reference genome GRCh38 using MiniMap2, as shown in FIGS. 2 and 3. Reads that map to the same genomic regions (within <5 bp) are grouped into read clusters to achieve batch computing. All reads belonging to same read-clusters and having UMIs within two LDs are considered to be amplified from same RNA molecules, thus their corresponding UMIs are curated to be identical. To overcome sequencing errors in single molecules, reads with identical UMIs are collapsed into consensus sequences using SPOA for better detection of point mutations in gene bodies. scNanoGPS then re-maps single cell consensus reads to generate single cell consensus BAM files. Lastly, to better facilitate scNanoRNAseq data analysis, the present invention has compiled existing long-read specific tools into scNanoGPS to form a complete pipeline to calculates gene expression, isoform expression and point mutation profiles of individual cells from single cell consensus BAM files. Furthermore, single cell copy numbers are calculated using previously published algorithm CopyKAT. These data can then be used to detect cell-type-specific isoforms and mutations as well as gene expressions to demonstrate the applications of scNanoGPS in investigating the underlying mechanisms of human diseases including but not limited to tumors.

To summarize, scNanoGPS achieves complete independence through iCARLO algorithm to detect true CBs and constructs consensus molecules from reads with same UMIs to accurately call mutations and count RNA molecules. On the contrast, both Sockeye and BLAZE relies on barcode whitelist and arbitrary cutoff to detect possible CBs, as shown in Table 1. While BLAZE doesn't support UMI detection, Sockeye UMI function depends on high quality of reads that filters out large portion of data. Moreover, scNanoGPS assembles variant detection pipeline together to call mutations from consensus reads. Taken together, scNanoGPS of the present invention provides a full spectrum of functional modules to analyze scNanoRNAseq data from raw FASTQ data to same-cell multi-omics profiles of thousands of cells.

Performance of scNanoGPS in Processing High Throughput scNanoRNAseq Data

To test the technical feasibility, the present invention has applied scNanoGPS to process the scNanoRNAseq data of two cancer cell lines, A375 and H2030. Strikingly, PromethION yielded 67.4 million reads of the A375 library on one flow cell and 105.3 million reads of the H2030 library on another flow cell.

This resulted in averaged depths of 15,944 reads per cell in A375 and 21,710 reads per cell in H2030, which were close to NGS scRNAseq depths, as shown in Table 2.

The median read lengths of both datasets were around 900 bp, consistent with the traces of pre-sequencing cDNAs, as shown in FIG. 4 and FIG. 12. The scNanoGPS results showed that most reads (A375: 86.80%, H2030: 87.31%) had the expected pattern of adaptor sequences. In total, the present invention detected 3,649 and 4,212 cells with averaged coverages of 2,688 and 3,553 genes per cell, respectively. Standard NGS 3′-scRNAseq (10× Genomics) data was generated from the same cDNA pools as previously described. Since 3′-scRNAseq (10× Genomics) was a widely used mature method and sequenced on Illumina sequencer (NovaSeq 6000) that has very low error rates in CBs and UMIs, the present invention treated these data as gold standard to benchmark scNanoGPS barcode detection accuracy. The analysis showed that scNanoGPS achieved high concordance (92%) with the standard 10× Genomics data in detecting true CBs, as shown in FIG. 5, with minor dis-concordance in thresholding low-quality cells that could be mitigated by secondary analyses, as shown in FIG. 13. scNanoGPS standalone achieved high accuracies in detecting true CBs, comparable to the performance of both whitelist-dependent approaches, i.e., BLAZE and Sockeye. While the time consumed in CB detection step were similar, scNanoGPS used less memories than BLAZE, according to Table 3. The present invention recorded time and space usages of each functional module of scNanoGPS when analyzing the example dataset, A375 in Table 4 to provide guidance of preparedness of computing resource.

Next, the present invention compared the number of UMIs detected by scNanoGPS to standard 10× Genomics data. The results revealed significantly high correlations (A375: Pearson's r=0.97, P-value <2.2e-16; H2030: Pearson's r=0.89, P-value <2.2e-16), according to FIG. 6. The gene detection rates per UMI were similar as well, although the numbers of UMIs per cell were fewer in long-read data compared to 10× Genomics data (A375: coef-34%; H2030: coef=56%) due to lower sequencing depths, as shown in FIGS. 6-7. The combination of CBs, UMIs and genes detected by scNanoGPS reached up to 72% (SD: 2%) concordance on average with NGS approach, confirming the reliability of barcoding detection results, according to Table 5.

In one embodiment, the present invention further compared single cell transcriptomes computed by scNanoGPS to standard 10× Genomics 3′-scRNAseq data. The results again showed that scNanoGPS achieved high concordance (A375: Pearson's r=0.89, P-value <2.2e-16; H2030: Pearson's r=0.91, P-value <2.2e-16) in measuring gene expression levels compared to standard 10× Genomics data, as shown in FIG. 8. Down-sampling analysis suggested that ˜15,000 long-reads per cell on average are needed to robustly profile single cell full-length transcriptomes, as shown in FIG. 14. There were only small fractions (A375: 0.92%; H2030: 0.79%) of detected genes showing significantly different expression levels (FDR-adjusted P-values <0.05, |log 2(Fold Changes)|≥1) between the two approaches according to FIG. 8. Of note, 10× Genomics 3′-scRNAseq showed a higher chance of detecting ribosomal genes, whereas scNanoGPS detected more long noncoding RNA (lncRNA) genes and pseudogenes, according to FIGS. 8-9. The lengths of scNanoGPS enriched genes were significantly longer than NGS enriched genes, A375: P-value=8.16×10⁻¹⁰; H2030: P-value=1.96×10⁻⁹, as shown in FIG. 10, which is suspected as due to minor fragmentation bias against longer genes in NGS library preparation as previously reported.

In one embodiment, to investigate whether long-read single cell transcriptome data could serve as a data source for inferring genome-wide DNA copy number alterations (CNAs), CopyKAT was run on H2030 which was known to have aneuploidy. As expected, the results showed that H2030 had genome-wide amplifications on Chr2, 3q, 5, 6q, 7, 8q, 14, 15p and deletions on Chr 4, 11p, in FIG. 11. The averaged pair-wised Pearson's correlation between the two approaches reached up to 94%, confirming the feasibility of inferring CNAs using scNanoRNAseq data.

In one embodiment, to evaluate whether scNanoGPS can robustly detect major cell types in human tumors, the present invention performed scNanoRNAseq on 4 frozen tumors collected from Renal Cell Carcinoma (RCC1, RCC2) and Melanoma (MEL1, MEL2) patients according to Table 2. The present invention performed unbiased clustering of all cells within each tumor as shown in FIG. 15 upper panel, and annotated epithelial cells having both aneuploidy and high expression levels of known cancer-type specific genes as tumor cells, as shown in FIG. 16. In consistence with previous studies, the present invention's data showed that tumor cells outcompeted normal epithelial cells in all 4 tumors due to stronger fitness advantage. The non-tumor cell type clusters were annotated using known cell-type markers. For fair comparisons, the same clustering and annotations were conducted on paralleled 10× Genomics data, as shown in FIG. 15 middle panel. The results confirmed high concordance between the two approaches, as shown in FIG. 15 lower panel, except that scNanoGPS rescued more lymphocytes that expressed far fewer genes than other cells in the same experiment.

To summarize, the present invention analyses showed that scNanoGPS reliably deconvoluted long-reads into single-cells and single-molecules to detect single cell transcriptomes and dissect the tumor microenvironment (TME) from scNanoRNAseq data. scNanoGPS enables detection of cell-type-specific splicing profiles in human tumors

In one embodiment, to access the robustness of scNanoGPS in discovering splicing isoforms of different cell types in the TME, the present invention performed detailed isoform analysis of a frozen kidney tumor (RCC1). On average, scNanoGPS detected 6 transcripts per gene by referring to all known transcripts in GENCODE (v32). Comparisons with bulk RNAseq (NGS) data of same tumor revealed that up to 47% isoforms were only detected by TGS approaches, as shown in FIG. 25 upper panel, while isoform expression levels showed moderate to high concordance: median: 96%; mean: 68%; as shown in FIG. 25 lower panel. Further analyses showed that each cell type tended to express a combination of multiple isoforms, consistent with a recent study that reported isoform specificity in mouse cortex. To identify cell-type-specific preference of isoforms, the present invention compared the relative compositions of different isoforms of each gene among all 7 major cell types. Analysis of the present invention identified 1,014 genes that preferably expressed significantly different combinations of isoforms (DCIs, Chi-sq test P-values<0.05 and |Prevalence Differences|≥ 10%) among all cell types, including 499 DCI genes in tumor cells, 122 in endothelial cells, 137 in fibroblasts, 90 in myeloid cells, 38 in T & NK cells, 90 in plasma B cells and 38 in follicular B cells, according to FIGS. 17-19, and Table 5-11. Of note, the present invention detected 2-4 times more genes with DCIs in tumor cells compared to immune and stromal cell types. The top-ranked tumor-cell-specific DCI genes were PDE10A and NR4A2 involved in cAMP pathways. Additionally, the present invention observed that tumor-cell-preferred isoforms had slightly more exons, paired T-test P-value=0.01, as shown in FIG. 20, particularly in genes with more than 20 exons such as NBPF10, VPS13C, and NBPF14, shown in FIG. 21. According to FIG. 22, Geneset (MSigDB, GO:BP) analysis showed that cell-type-specific DCI genes were commonly enriched in pathways related to cell-type-specific functions, such as cAMP pathway and resistance associated glucuronidation pathway in tumor cells, interferon-alpha production pathways in myeloid cells, lymphocyte proliferation pathway in lymphocytes, and immunoglobin-mediated immune responses in B cells.

Notably, the present invention observed that a large portion (mean: 83%, SD: 9.9%) of cell-type-specific DCI genes were not detected as differentially expressed genes (DEGs) in all cell types, as shown in FIG. 18. Tumor-cell-specific DCI genes preferably expressed different most dominant transcripts (MDTs) regardless of their overall gene expression levels. For instance, the proliferation gene PPRPFL, expressed MDTs ENST00000303202 in tumor cells and ENST00000399653 in normal cells, although the overall expression levels of this gene in the two cell types were not significantly different, as shown in FIGS. 26-27 and FIG. 23. On the other hand, the organelle hook protein coding gene HOOK2 preferably expressed ENST00000589134 in tumor cells and ENST00000593143 in normal cells and had significantly higher expression levels in tumor cells compared to normal cells, as shown in FIG. 23 and FIG. 27. In addition, the present invention observed a small fraction of cell-type-specific DCI genes that expressed same MDTs, but their cellular fractions were different between tumor and normal cells. One example was CYB5A which expressed isoform ENST00000340533 in 60% of tumor cells but 91% of normal cells. Another example was HMGN3 which expressed isoform ENST00000620514 in 94% of tumor cells but 66% of normal cells, as shown in FIG. 24 and FIG. 28.

Interestingly, the present invention also observed isoform preference in immune and stromal cell types, although fewer genes were involved compared to tumor cells. For instance, myeloid and T cells both expressed HAVCR2, yet the MDTs of this gene were distinct in the two immune cell types, as shown in FIG. 23 and FIG. 27. In contrast, the B cell specific gene, IGHG1 expressed the same MDT (ENST00000390549) in both follicular and plasma B cells, but the cellular prevalence of this MDT were significantly different in the two B cell subtypes (72% in follicular B cells, 99% in plasma B cells), as shown in FIG. 24, indicating its relevance to sub-cell-type specific functions.

In summary, the present invention demonstrated the usages of scNanoGPS in studying cell-type-specific splicing isoforms in tumors. The results showed that a larger portion of genes utilized different MDTs in both tumor and immune cell types. Genes expressing same MDTs may have distinct cellular prevalence in different cell types regardless of overall gene expression levels.

Transcriptome-Wide Mutations of Different Cell Types in the Tumor Microenvironment

To accurately detect transcriptome-wide mutations in single cells, the present invention built consensus sequences of single molecules and required at least 2 variant supporting reads. In addition, the present invention filtered out mutations that were detected in less than 1% of cells over all cells or less than 5% within individual cell types, which removed most random errors, as shown in Table 13. In total, the present invention detected 6,390 mutations from 3,470 single nuclei transcriptomes of a frozen kidney tumor (RCC1). Further, in one embodiment, the present invention classified these mutations into germline mutations detected in >90% cells with coverages and somatic mutations detected in a wide range of cells across all cell types, as shown in FIGS. 34-35. The analysis showed that 90.6% germline mutations aggregated from scNanoGPS results were detected in pseudo-bulk long-read data, as shown in FIG. 36. However, the concordance between TGS with NGS were moderate (germline: 46.5% and somatic: 39.8%), which largely were due to differences in gene body coverages, e.g., NGS bulk RNAseq data had poorer uniform gene body coverages, as shown in FIG. 37. Among all mutations, 17.9% were in exonic regions, while others were spreading across non-coding regions (35.8% intronic, 28.8% intergenic, 3.7% 5′UTR and 13.8% 3′UTR), as shown in FIG. 29. Statistical analysis revealed that the odds of mutation detection were significantly (all chi-sq P-values <2.2e-16) increased in exonic (odds ratio: 7.8), 5′-UTR (odd ratio: 5.2) and 3′-UTR (odds ratio: 4.8) regions and decreased in intergenic (odds ratio: 0.65) and intronic (odds ratio: 0.46) regions as expected. Of note, it was observed 53.3% exon mutations were nonsynonymous. According to FIG. 30, transition mutations were more frequent (13-15%) than transversion mutation types (4-6%). The potential RNA editing (C>U) events were not distinguishable from C>T transition (15.3%). The data of the present invention showed that these transcriptome-wide mutations were distributed across all chromosomes except for Y-chromosome, as shown in FIG. 31. The data revealed 4 shared mutation hotspots on Chr2, 6, 14 and 22 in all 7 cell types, where Chr6 mutation hotspot was known to affect HLA gene clusters in both tumor and normal development26 and Chr14 hotspot harbored mutations in LncRNAs, according to FIGS. 38-39.

In one embodiment, the data showed that the mutated transcripts were detected in 15-25% cells of each cell type, according to FIG. 34, indicating the dependency of mutation detection rates on gene expression levels and transcript capture efficiency in single cell RNA sequencing technology. The number of total mutations in individual cells varied across cell types from 1119 SNVs in tumor cells, 758 in endothelial cells, 506 in fibroblasts, 703 in myeloid, 461 in lymphoid, 484 in plasma cells and 501 in follicular B cells, as shown in FIG. 40. To mitigate false positive detection of mutations due to ambient RNAs, SoupX30 was run to de-noise the data and removed mutations that were either landed on ambient RNAs or mapped to non-coding regions, as shown in FIGS. 41-42. Next, the present invention compared the cellular frequencies of mutations among different cell types to identify mutations that were differentially expanded (deMuts). The results showed that tumor cells had the largest number (N-609) of deMuts, followed by myeloid cells (N=99), plasma B cells (N=63), endothelial cells (N=57), follicular B (N=29), lymphocytes (N=26) and fibroblasts (N=1), according to FIG. 32 and Table 14-20. Observation of lower mutation burden in normal cell types is consistent with the prior knowledge of spontaneous mutation accumulation in normal organs throughout life time. Tumor cell specific deMuts included many COSMIC genes, such as VEGFA, NEAT1, MALAT1, HILPDA etc., as shown in FIG. 33. Further, the present invention detected several genes that were both mutated and differentially spliced in the same cell types, such as HMGN3 and UBE2G2 in tumor cells, CD74 and IFI30 in myeloid cells, and RPS2 in endothelial cells indicating their important roles in tumorigenicity. Additionally, it is observed several cell-type-specific deMuts involved in the spliceosome (GSEA: KEGG pathway) such as SRSF3 and HNRNPC mutants in tumor cells and DDX5 mutant in endothelial cells, according to FIG. 40, lower panel.

In summary, the present demonstrated that scNanoGPS can robustly detect cell-type and cell-state specific mutations. The data highlighted the importance of identifying population-specific mutations to understand their functional roles in cancer progression.

Conclusion

In one embodiment, the present invention discloses a computational tool called scNanoGPS to facilitate high throughput single cell long-read sequencing data analysis. scNanoGPS achieves independent deconvolution of raw data without the guidance of short-reads nor barcode whitelist and calculates genotypes-phenotypes of thousands of individual cells, addressing the major computational challenges of an emerging scNanoRNAseq technology.

Two previous methods called Sicelore and scNapBar were developed to deconvolute raw reads, however, both relied on the guidance of paralleled NGS data. An experimental method called scCOLOR-seq was developed to reduce error rates in barcodes by designing bi-nucleotide repeats in barcode sequences, but it still relied on prior knowledge of true barcodes to curate errors. Moreover, scCOLOR-seq required customized synthesis of gel-beads to adapt to the droplet system. The other NGS-independent barcode deconvolution tools, Sockeye and BLAZE relied on comparison of long-reads data with barcode whitelist and large LDs. This is worrisome due to barcode manufacturing deviations and misassignment introduced by large LDs. In comparison, scNanoGPS achieves >80% accuracy of detecting true CBs directly from long-reads data using a multi-step method, iCARLO, without guidance of NGS data nor barcode whitelist. Sockeye also provided UMI detection function, which unfortunately dropped off a large portion of data due to over-filtering and/or over-merging.

In one embodiment, dysregulation of transcript isoforms plays critical roles in tumor progression. Previous studies showed that only ˜20-40% of transcriptional isoforms can be reconstructed from NGS bulk RNAseq data. Long-read single cell sequencing technologies enable in-depth annotation of splicing isoforms at single cell levels. scNanoGPS provides a robust computational tool to achieve this goal. The disclosed analysis of a frozen kidney tumor revealed that all major cell types in the tumor commonly express a combination of different isoforms instead of one canonical isoform.

In one embodiment, another important finding is that tumor cells preferably express different MDTs of tumor suppressors although their overall gene expression levels are not significantly different from normal cells. The results implied that the discovery of cancer-specific genes using gene expression levels may only be revealing the tip of iceberg of transcriptional diversities in cancer.

scNanoGPS provides a powerful approach for synchronic tracing of cell-lineage and cell-fate by measuring both plastic phenotypic markers (genes, isoforms) and stable genetic markers (mutations, copy numbers) of the same cells to study tumor evolution and therapeutic responses. scNanoGPS detects transcriptome-wide point-mutations with accuracy by building consensus sequences of single molecules and performing consensus filtering of cellular prevalence, which removes most false calls due to random sequencing errors.

In addition, scNanoGPS has broad applications in many other genomic areas, such as measuring single cell gene fusions, tandem repeats, splicing velocities, repetitive genes, or long non-coding genes to investigate diverse mechanisms of human diseases including but not limited to cancer.

In one embodiment, the present invention also discloses scNanoFUSE, which provides several methods for detecting fusion reads, removing false positives, predicting accurate fusion location, and assembling the consensus fusion transcripts.

Data and Software Availability

Raw sequencing data and processed data are submitted to Gene Expression Omnibus (GEO): GSE212945. Software is available at GitHub: github.com/gaolabtools/scNanoGPS Codes of statistical tests are provided in the Supplementary Note.

Methods Cancer Cell Line and Tumor Tissue Samples

The two cancer cell lines (A375, H2030) and the four frozen tumors (RCC1, RCC2, MEL1, MEL2) were provided by Dr. Jason Huse at UT MD Anderson Cancer Center. The cell lines are standard commercialized cancer lines. The tumor tissues were collected with consent under IRB approval at UT MD Anderson Cancer Center.

Preparation of Single Nucleus Suspension

Single nucleus suspensions of two cancer cell lines were prepared by following the 10× Genomics protocol (CG000365 Rev C), which is hereby incorporated by reference. Single nucleus suspensions of frozen tumor tissues were prepared according to the method as previously described. Frozen tissue was cut into tiny pieces in 10-cm Petri dish with 500 ul-2 ml NST-DAPI buffer for 10-15 minutes and filtered through a 40 mm Flowmi into 1.5 ml LowBind Eppendorf tube and centrifuged at 4° C. 300 g for 5 minutes. The resulting nuclei pellet was washed three times with cold Nuclei Wash and Resuspension Buffer. After cell counting, the nuclear suspension was centrifuged again and resuspended in the appropriate volume depending on the nuclei counting results. Preparation of NST-DAPI buffer: Mix 800 ml of NST solution (146 mM NaCl, 10 mM Tris-base (pH 7.8), 1 mM CaCl2, 21 mM MgCl2, 0.05% (wt/vol) BSA and 0.2% (v/v) Nonidet P-40) with 200 ml of DAPI solution (106 mM MgCl2 and 10 mg of DAPI). The solution is filter-sterilized and is stored at 4° C. in the dark for up to 1 year. Preparation of Nuclei Wash and Resuspension Buffer: 1×PBS with 1.0% BSA and 0.2 U/ul RNase Inhibitor.

Preparation of Barcoded Full-Length cDNAs of Single Nuclei

Single nucleus suspensions were loaded onto 10× Genomics Chromium Controller (iX) with Chip J to capture 3000-6000 single nuclei. The full-length mRNAs and/or pre-mRNAs were barcoded with cell barcodes (cellBCs) and unique molecular identifiers (UMIs) through cDNA amplification using 10× Genomics protocol. In one embodiment, the present invention modified the cDNA amplification protocol by extend the elongation time to 3 minutes to enrich longer molecules as previously described.

The barcoded full-length cDNA transcripts (10 ng) were amplified for five cycles with the following two customized primers synthesized by Integrated DNA Technologies (Coralville, IA): 1) 5′-biotinylated TruSeq Read 1 forward primer 5′-/5Biosg/AA AAA CTA CAC GAC GCT CTT CCG ATC T-3′ (25 nM); 2) 3′ partial TSO reverse primer 5′-NNN AAG CAG TGG TAT CAA CGC AGA GTA CAT-3′. The amplified single-cell cDNA transcripts were subjected to 0.8×SPRIselect reagent (Beckman Coulter, CA) clean-up to remove unbound and excess biotinylated primers, where the bound cDNA was eluted off the bead matrix in 45 uL of Qiagen Buffer EB (Qiagen; Valencia, CA). The eluted cDNA was further purified through the binding of the biotinylated template to Dynabeads™ M-270 Streptavidin beads (Invitrogen; Waltham, MA). Prior to the selection of the cDNA template, 15 uL of the Dynabeads™ M-270 Streptavidin beads were washed in 1 mL of 1×SSPE solution (UltraPure™ 20×SSPE Buffer (Invitrogen) freshly prepared with nuclease-free water). A magnetic stand was used to separate the streptavidin beads from the initial wash solution. The streptavidin beads were then washed three times with 15 uL of 1×SSPE with removal from the magnet and resuspension of the beads in a fresh wash solution with each repeat wash. Following the final wash, the streptavidin beads were resuspended in 10 uL of 5×SSPE solution and the biotinylated cDNA template was added to the washed beads. This mixture was placed on a tube rotator at room temperature for 15 minutes. Post incubation on the rotator, with the biotinylated cDNA template bound to the washed streptavidin beads, the sample was placed back on the magnetic stand for separation. The cDNA-bound beads were washed twice with 100 uL 1×SSPE solution and a final wash with 100 uL Buffer EB. The use of the biotinylated forward primer and subsequent purification with the Dynabeads™ M-270 Streptavidin beads allowed for the selective depletion of cDNA missing the terminal poly(A)/poly(T) tail.

The streptavidin beads containing bound biotinylated cDNA were then resuspended in 100 uL of PCR master mix for a secondary amplification for five cycles with regular PCR primers: 1) TruSeq read 1 forward primer 5′-NNN CTA CAC GAC GCT CTT CCG ATC T-3′ and 3′ partial TSO reverse primer 5′-NNN AAG CAG TGG TAT CAA CGC AGA GTA CAT-3′. The PCR amplified product was purified with 0.8×SPRIselect reagent into a final elution of 51 uL in Buffer EB to allow for adequate template/volume for the necessary assessment of quality control (QC) metrics and PromethION library preparation.

The KAPA Biosystems HiFi HotStart PCR Kit (Roche; Basel, Switzerland) was used to prepare all PCR amplification mixtures for Nanopore library preparations. The following PCR conditions were followed for amplification: initial denaturation, 3 mins at 95° C.; five cycles of denaturation for 30 secs at 98° C., annealing for 15 secs at 64° C., and extension for 5 mins at 72° C.; followed by a final extension for 10 mins at 72° C.

Nanopore Sequencing Library Preparation for Full-Length cDNAs

Based on the sample molarity and average cDNA transcript length derived from the QC metrics, the sample input volume was calculated and used to progress into nanopore library preparation. The SQK-LSK110 ligation sequencing kit (Oxford Nanopore) was used to generate PromethION long-read cDNA libraries. The final sequencing was run on PromethION flow cell (v9.4.2) with one sample per flow cell by the DNA technology core at UC Davis using R9.4 chemistry. The output data was base-called live during the run using base-caller guppy (v5.0.12) in super-accurate base-calling model.

Generating Benchmarking Data with Paralleled NGS Sequencing of Fragmentized cDNAs

The same aliquot (25% volume) of barcoded full-length cDNAs were fragmented and subjected to next generation sequencing library preparation by following 10× Genomics Next GEM Single Cell Gene Expression protocol. The final libraries were sequenced on the Illumina Novaseq 6000 sequencer at the NUcore at Northwestern University. The sequencing data were processed using 10× Genomics software CellRanger ARC (v2.0)29.

Quality Control of scNanoRNAseq Data

The raw FASTQ files were processed by the scNanoGPS ‘NanoQC’ function to scan the distribution of raw read lengths, which generated a PNG plot named ‘read_length.png’ and a tab-separated table named ‘read_length.tsv’. The first and last 100 nucleotides of raw reads were extracted for sequencing quality analysis FastQC39.

Scanning Long-Reads with Expected Adaptor Patterns

The raw FASTQ files were processed by scNanoGPS ‘Scanner’ function to scan the expected adaptor patterns using the parameters (match: 2, mismatch: −3, gap opening: −5, gap extension: −2, sequence identity ≥70%) equivalent to NCBI Basic Local Alignment Tool (BLAST) algorithm. Raw reads with insert length less than 200 bp were excluded from scanning. After ‘Scanner’, a compressed file containing parsed raw cell barcodes (CBs) named ‘barcode_list.tsv.gz’ and a filtered read sequence file named ‘processed.fastq.gz’ were generated.

Deconvolution of Long-Reads into Single Cells

The raw reads that passed QC and pattern filtering steps were demultiplexed into single cells using scNanoGPS ‘Assigner’ function. Another input was the parsed barcode lists generated by ‘Scanner’. The true list of CBs was retrieved through an integrated algorithm called iCARLO. This algorithm included 4 steps. First, all CBs were ordered decreasingly by the number of supporting reads. The number of supporting reads and the order index were transformed into log 10 scale, as shown in FIG. 2, step 3 Assigner. The raw list of true CBs was estimated by thresholding the maximal partial derivatives of supporting reads against the rank of CBs. To buffer the changes, the present invention smoothed the partial derivatives within each 0.001 window of log 10-scaled CB ranks.

Let X be the number of supporting reads of CBs, i be the rank order of all CBs, and w be the 0.001 smoothing windows as defined in equation (1).

$\begin{matrix} \forall X, i, w \in (1, 2, 3, \dots, N) & (1) \end{matrix}$

Each window w contains a set of CBs, allowing empty, according to their rank order i in log 10 scale per 0.001 tick as shown in equation (2).

$\begin{matrix} 0.001 \times w < \log_{10} i < 0.001 \times (w + 1) & (2) \end{matrix}$

The partial derivatives were calculated for each CB and then smoothed by taking median average of all values within each window w as shown in equation (3). The crude anchoring point was defined as a threshold cutoff where a smoothing window w had maximal partial derivative. The present invention defined the raw number of CBs (Cr) at this crude anchoring point as follows:

$\begin{matrix} Cr = 10^{0.001 \times \arg_{w} \max {med}_{i} [\frac{\log_{10} X_{i + 1} - \log_{10} X}{\log_{10} (i + 1) - \log_{10} i}]} & (3) \end{matrix}$

The present invention then extended this crude anchoring point by an empirical percentage (10%) to an external boundary (N=Cr2) of true CBs to rescue as many CBs as possible. In the third step, the present invention exhaustively computed pair-wised LD distances of all Cr2 CBs. CBs within 2 LD were merged back one directionally to the CB harboring more supporting reads and obtained a collapsed list of true CBs (N=Cr3). Lastly, the present invention retrieved the final list of true CBs (N=Cr4) by removing CBs which cover less than 300 genes. According to this final list of true CBs, the master FASTQ file resulting from ‘Scanner’ was split into single cell FASTQ files stored in a temporal folder holding all the meta files for further usages.

Curation of Sequencing Errors in Single Molecules

Detection of the true list of UMIs and curation of sequencing errors in single molecules was performed by using scNanoGPS ‘Curator’ function. To detect true UMIs, deconvolution of the single cell FASTQ files were first aligned to the reference genome (GRCh38) using MiniMap2 under the splicing mode (−ax splice). Reads mapped to the same genomic regions (coordinates within 5 bp) were grouped into batches. Batch calculation was conducted by paralleled computing cores. For the reads, that belong to the same clusters and have UMI within two LDs, were considered as reads of the same molecules. These UMI sequences were curated to be identical. To curate errors in gene bodies, the reads with the same curated UMIs were collapsed into consensus sequences of single molecules using SPOA13. Finally, the consensus reads were re-mapped against the reference genome (GRCh38) using MiniMap2 under splicing mode (−ax splice) to generate consensus BAM files for all downstream analyses.

Calculation of Same-Cell Multi-Omics from Consensus Reads

The consensus BAM files of single cells were used as input to calculate single cell multi-omics, including transcriptomes, isoforms and point mutations using scNanoGPS ‘Reporter’ functions. The single cell gene expression profiles were calculated using FeatureCount from the Subread package that support long-read gene level counting. The single cell isoforms were calculated using LIQA, a method designed to calculate isoforms from long-read data of spliced mRNAs. The present invention used default parameters (weight of bias correction: 1, maximal distance: 20 bp) when running LIQA. The single cell point mutation detection was conducted using a robust long-read variant detection tool, LongShot. The present invention benchmarked the LongShot results in terms of the number of SNVs with different cell prevalence and different number of supporting reads, as shown in Table 1. With the elbow method, the present invention required that each alternative allele should be supported by at least two consensus reads. The resulting VCF files of single cells were merged using BCFtools (v1.15). A final list of mutations of all cells was obtained by consensus filtering, where all variants detected in less than 1% of the cells were considered as random errors and removed from analysis. To differentiate between true wild-types and missing values, the present invention re-scanned the read depths of all loci in the final list by Samtools (v1.15). Loci with 0/0 genotypes with supporting reads >0 were defined as true wildtypes, otherwise, as missing values if none supporting reads were found. The final mutation loci were annotated using ANNOVAR, which included dbSNP (v150) and COSMIC (v96) as references. The single cell copy numbers were calculated using the previously published method, ‘CopyKAT’ (v1.0.6) with default parameters, which is hereby incorporated by reference. In all final output matrices, features/genes were put in rows while cell barcodes were in the columns.

Single Cell Gene Expression Data Analysis: QC and Defining Major Cell Types

The gene expression matrices of NGS-based 3′-scRNAseq data were processed using CellRanger ARC29 (10× Genomics) and sent for downstream analysis using the ‘Seurat’ R package (v4.1.1). In 4 tumor samples, doublets were removed using R package ‘DoubletFinder’ (v2.0.3) with an assumed doublet rates of 0.8% per 1000 cells. Cells with more than 10,000 genes, or more than 100,000 UMIs, considered as doublets, were removed as outliers and suspected doublets. Cells with less than 300 genes were filtered out due to low gene coverages. Furthermore, cells with extremely higher fractions of mitochondrial genes were filtered out using arbitrary outlier cutoffs (5% in RCC1 and RCC2; 20% in MEL1 and MEL2). UMI count matrices were normalized using ‘LogNormalize’ method in ‘NormalizeData’ function and scaled across cells using ‘ScaleData’ function in ‘Seurat’. The top 2000 highly variable genes were selected with ‘FindVariableFeatures’ function based on ‘vst’ method and used for Principal Component Analysis (PCA). Next, the present invention performed PCA and uniform manifold approximation and projection (UMAP) for dimension reduction with the top 30 PCs. ‘FindNeighbors’ function based on the top 30 PCs and ‘FindClusters’ functions were applied to perform unbiased clustering of cells. In all the samples, the present invention defined a cluster of low-quality cells that did not express known cell type markers and had much fewer genes and UMIs compared to other cells in the same experiments. Final clustering analyses were reperformed without these low-quality cells. To identify the tumor cells, the present invention used the UMI count matrix as input to infer chromosomal CNA profiles using R package ‘CopyKAT’ (v1.0.6). Cells with genome-wide CNAs were labeled as tumor cells.

Analyses of Cell-Type-Specific Splicing Isoforms

Cell-type-specific splicing isoform analyses started from single cell isoform expression matrices that summarized the expression levels of all known isoforms based on GENCODE (v32) in single cells. For pairwise two group (cell type) comparisons, the present invention filtered out sporadically expressed genes, which were detected in less than 5% cells in both comparison groups. Additionally, genes with only one isoform were excluded from this analysis. To mitigate false discovery driven by dropouts, the isoform expression levels of a given gene were aggregated across all cells within each comparison group. The aggregated counts of expressed molecules of all isoforms of a given genes in both comparison groups were sent for Chi-square tests to test whether the relative composition of different isoforms of a given gene was significantly different in the two comparison groups or not. P-values were adjusted using Benjamin-Hochberg (BH) correction for multiple testing with a 5% false discovery rate. The relative frequency of all isoforms of a given genes and the differences in two comparison groups were also calculated. Finally, the genes expressing significantly different combination of isoforms (DCIs) were defined as having FDR adjusted P-values <0.05 and at least one isoform had different cellular prevalence in two comparison groups >=10%.

Detection of Cell-Type-Specific SNVs

To further remove random errors, the present invention filtered out the called positions that were detected in less than 5% cells in each cell type or in comparing group. Next, the present invention generated a count matrix that included the number of wild-type cells (expressed only reference alleles) and the number of mutated cells (expressed variant alleles) of all candidate SNVs in both groups that were under comparison. In one embodiment, the present invention performed a Chi-square test to measure whether a candidate SNV had significantly different cellular frequencies. P-values were adjusted using BH correction for multiple testing with a false discovery rate of 5%. This analysis was only performed using data of cells that had read coverages. Cells without read coverages were not included to calculate the cellular frequencies of mutations. It was determined the candidate SNVs with FDR adjusted P-value <0.05 and the differences in cellular frequencies >0.1 as population specific differentially expanded SNVs (deMuts).

Summary of Statistical Methods

The present invention applied Chi-sq tests to compare the relative frequencies of isoforms or mutations in two comparison groups. P-values were adjusted using BH method to adjust for multiple test errors with a false discovery rate of 5%. Pearson's correlation and P-value were applied to measure the similarity of gene expression profiles and the total counts of UMIs per cell between scNanoGPS and NGS approaches. Paired-two-side t-test was performed to compare the number of exons of different isoforms of same genes between tumor and normal cells. All significance cutoffs used in this study were set at 0.05.

TABLE 1 Comparison of functional modules of scNanoGPS with existing tools Function modules scNanoGPS Sockeye BLAZE Detection of true Scan possible CBs from raw data using 4-step Compare to barcode whitelist to Compare to barcode CBs iCARLO algorithm; Curate errenous CBs with identify possible CBs, allowing 2 whitelist to identify possible top-ranked CBs (having more supporting Levenshtein Distance CBs, allowing 5 Levenshtein reads); allowing 2 Levenshtein Distance Distance Detection of UMIs Mapped to same genomic positions (<5 bp), Network-based clustering, N/A, by the time of allowing 2 Levenshtein Distance allowing 2 Levenshtein Distance submission Construction of Collapse reads with same UMIs N/A, by the time of submission N/A, by the time of consensus reads submission Expression profile Output gene by cell matrix Output gene by cell matrix Output gene by cell matrix calculation Isoform profile Output isoform by cell matrix N/A, by the time of submission Output isoform by cell calculation matrix SNV profile Output SNV by cell matrix N/A, by the time of submission N/A, by the time of calculation submission

TABLE 2 Sample quality metrics Pass Aver- Aver- Reads rates aged aged past of quality Detected number Inter- Sam- adaptor adaptor Median Maximal score number of raw Exonic Intronic genic ple Cancer Patient Total pattern pattern read read in first of reads mapping mapping mapping ID type ID reads scanning scanning length length 100 bp cells per cell rates rates rates RCC renal RCC 98,349,656 76,630,302 77.92% 921 bp 190,885 bp 20.47 3,470 22,084 13.91% 70.20% 15.89% 1 cell 12 carcinoma RCC renal RCC 87,800,959 68,137,378 78.83% 830 bp 386,890 bp 20.91 3,638 18,729 15.50% 69.95% 14.55% 2 cell 12 carcinoma- brain metastasis MEL melano- MEL 72,828,041 55,845,250 78.04% 890 bp 183,138 bp 21.05 7,426 7,520 12.65% 65.92% 21.43% 1 ma 3 MEL melanoma- MEL 98,363,542 75,990,450 78.47% 801 bp 637,942 bp 20.93 1,192 63,750 13.67% 59.620% 26.71% 2 brain 3 metastasis H20 non- NA 105,347,205 91,444,891 87.31% 856 bp 411,989 bp 21.81 4,212 21,711 11.84% 59.84% 28.32% 30 small cell carcinoma A37 melanoma NA 67,436,356 58,178,979 86.80% 899 bp 282,743 bp 21.75 3,649 15,944 11.91% 54.91% 33.18% 5

TABLE 3 Comparison of CB detection results of three tools A375 H2030 Items scNanoGPS BLAZE Sockeye scNanoGPS BLAZE Sockeye True positive rate* 97.99% 95.84% 95.85% 98.32% 97.27% 97.23% False positive rate# 2.01% 4.16% 4.15% 1.68% 2.73% 2.78% True negative rate 99.91% 99.94% 99.94% 99.93% 99.97% 99.97% False negative rate 0.90% 0.60% 0.60% 0.70% 0.30% 0.30% F1 score 0.90 0.92 0.92 0.93 0.96 0.96 Time (H:M:S) 18:17:17 5:01:37 15:37:25 28:02:05 9:40:45 23:10:01 Peak memory 1.21 GB 7.14 G NA 1.30 GB 8.31 GB NA *detected CBs existed in CellRanger list/total detected CBs #detected CBs not existed in CellRanger list/total detected CBs

TABLE 4 Usages of computing resources of analyzing A375 data with scNanoGPS Function Time spent Memory Storage Modules* (H:M:S) usage usage Input FastQ files — — 89 Gb Scanner 18:17:17.84 1.30 Gb 52.87 Gb Assigner 18:29:35.18 576 Gb 86.50 Mb Curator 53:15:38.13 16.82 Gb 74.69 Gb∧ Reporter_expression 01:49:48.48 6.64 Gb 11.65 Mb Reporter_isoform 11:01:22.24 2.76 Gb 23.81 Mb Reporter_SNV 04:38:56.50 42.55 Gb 12.27 Mb *with 30 cores; ∧temporary files

TABLE 5 Concordance of combinations of CBs, UMIs and genes of scNanoGPS results with CellRanger Total Overlapped combi- combi- nations nations of UMIs of Con- and UMIs and cord- Genes Genes ance Cells (counts) (counts) (%) CCTGCTCCATTAGGCC 6307 4423 70% (SEQ ID No. 1) GAGCTTAGTTCGCTCA 7541 5288 70% (SEQ ID No. 2) TTGATGTCATTGTCCT 7995 5567 70% (SEQ ID No. 3) AGCGGATAGCAGCTAT 11327 7718 68% (SEQ ID No. 4) GAGCGGTCAGGCTGTT 14193 9889 70% (SEQ ID No. 5) GATAACGAGCTGTAAC 14696 10103 69% (SEQ ID No. 6) GCCATGATCACCAATA 19799 13542 68% (SEQ ID No. 7) CGCTTAACAGGTCCTG 37762 26986 71% (SEQ ID No. 8) GCAACAGCATGTCAGC 58376 41477 71% (SEQ ID No. 9) GTATGTGGTATTGAGT 101695 75438 74% (SEQ ID No. 10) Combinations of CBs, 279691 200431 72% UMIs and Genes (counts)

TABLE 6 Tumor cell specific DCI genes Pre- Pre- Pre- Pre- Pre- Pre- valance valance valance valance valance valance of tumor of tumor differ- of of differ- cell cell ence normal normal ence pre- pre- of cell cell of Tumor ferred ferred tumor Normal preferred preferred normal cell isoform isoform cell cell isoform isoform cell preferred in in non- pre- preferred in in pre- FDR Gene isoform tumor tumor ferred isoform tumor normal ferred adj Name ID cells cells isoform ID cells cells isoform P-values P-values HMGN3 ENST00000620514 94.40% 66.35% 28.05% ENST00000275036 3.74% 21.62% 17.88% 4.66E−285 1.81E−281 HOOK2 ENST00000589134 46.97% 7.08% 39.88% ENST00000593143 24.43% 87.80% 63.36% 1.62E−150 3.16E−147 CYB5A ENST00000583418 30.62% 6.87% 23.74% ENST00000340533 60.49% 90.61% 30.12% 1.13E−101 1.46E−98 PPDPFL ENST00000303202 52.78% 5.68% 47.10% ENST00000399653 39.65% 73.78% 34.13% 4.20E−97 4.08E−94 CALD1 ENST00000361675 65.14% 45.60% 19.53% ENST00000475772 0.11% 10.91% 10.80% 1.19E−96 9.28E−94 HSPB1 ENST00000447574 32.17% 6.20% 25.96% ENST00000248553 67.27% 93.73% 26.46% 1.15E−65 6.40E−63 KCP ENST00000460528 38.23% 5.49% 32.73% ENST00000492679 29.52% 84.98% 55.46% 6.06E−60 2.94E−57 ARHGAP24 ENST00000264343 61.08% 28.84% 32.24% ENST00000509709 2.49% 23.57% 21.08% 3.49E−54 1.51E−51 CHCHD3 ENST00000262570 19.35% 7.36% 11.99% ENST00000457942 48.29% 86.22% 37.93% 7.78E−45 3.02E−42 MT1E ENST00000330439 51.21% 7.64% 43.57% ENST00000306061 45.54% 91.67% 46.13% 2.61E−44 9.24E−42 MT1X ENST00000568370 49.67% 8.87% 40.80% ENST00000394485 49.87% 89.24% 39.37% 6.62E−42 2.14E−39 DST ENST00000361203 10.68% 2.72% 7.95% ENST00000340834 11.44% 31.73% 20.30% 1.22E−41 3.64E−39 MSR1 ENST00000518343 54.18% 3.70% 50.48% ENST00000381998 7.77% 56.75% 48.98% 1.96E−41 5.43E−39 GLRX ENST00000507605 54.25% 13.77% 40.48% ENST00000237858 16.89% 62.12% 45.23% 3.10E−39 8.04E−37 VPS13C ENST00000249837 42.96% 22.43% 20.53% ENST00000559119 15.14% 33.85% 18.72% 4.65E−36 1.13E−33 ERN1 ENST00000433197 63.83% 39.97% 23.86% ENST00000606895 12.21% 37.42% 25.21% 2.87E−34 5.58E−32 UQCRH ENST00000486951 21.14% 2.95% 18.19% ENST00000311672 65.48% 88.55% 23.07% 2.56E−34 5.58E−32 RPS28 ENST00000449223 18.02% 4.91% 13.11% ENST00000600659 75.51% 93.47% 17.97% 2.85E−34 5.58E−32 C15orf48 ENST00000558632 48.63% 12.27% 36.36% ENST00000396650 31.58% 56.77% 25.19% 4.17E−32 7.71E−30 MRPS6 ENST00000483977 43.06% 26.69% 16.37% ENST00000399312 20.09% 55.96% 35.87% 8.21E−31 1.45E−28 NBEAL1 ENST00000449802 36.60% 13.83% 22.78% ENST00000460355 50.32% 76.70% 26.38% 2.67E−30 4.52E−28 HSPA4L ENST00000296464 16.87% 2.88% 13.99% ENST00000515262 78.52% 96.50% 17.98% 1.06E−29 1.72E−27 ENTPD1- ENST00000427300 20.39% 4.54% 15.85% ENST00000416301 27.20% 59.48% 32.28% 1.22E−29 1.90E−27 AS1 PLCG2 ENST00000564138 91.72% 69.27% 22.45% ENST00000563193 1.27% 8.73% 7.46% 8.59E−28 1.28E−25 ACAP1 ENST00000575415 31.89% 4.98% 26.91% ENST00000576628 24.22% 74.90% 50.68% 1.24E−26 1.78E−24 PDE4B ENST00000371048 28.42% 7.98% 20.43% ENST00000534463 10.27% 30.45% 20.18% 1.76E−26 2.45E−24 ARHGEF10 ENST00000495593 27.21% 5.75% 21.46% ENST00000375408 9.69% 84.02% 74.33% 7.92E−25 1.06E−22 L NDRG1 ENST00000414097 37.40% 22.02% 15.38% ENST00000519278 15.49% 31.68% 16.19% 1.33E−23 1.73E−21 TC2N ENST00000435962 34.51% 8.03% 26.48% ENST00000555302 9.57% 61.36% 51.79% 7.84E−23 9.52E−21 CSTB ENST00000640406 17.35% 5.90% 11.45% ENST00000291568 77.39% 92.29% 14.89% 1.28E−22 1.51E−20 UGT1A1 ENST00000360418 43.08% 16.69% 26.39% ENST00000305208 56.92% 83.31% 26.39% 2.17E−22 2.48E−20 RPS21 ENST00000370562 18.32% 5.14% 13.18% ENST00000343986 79.05% 93.63% 14.58% 1.80E−21 1.94E−19 PGF ENST00000555253 58.99% 31.64% 27.35% ENST00000238607 22.19% 46.77% 24.58% 4.14E−21 4.35E−19 TNFAIP8 ENST00000504771 28.35% 9.76% 18.58% ENST00000513374 30.88% 69.40% 38.52% 4.45E−21 4.56E−19 WSB1 ENST00000467843 52.01% 27.44% 24.58% ENST00000262394 25.88% 33.24% 7.36% 8.57E−21 8.54E−19 UGT1A6 ENST00000406651 23.18% 10.26% 12.93% ENST00000305139 61.53% 86.71% 25.18% 2.07E−20 2.01E−18 UGT1A10 ENST00000373445 35.42% 14.73% 20.69% ENST00000344644 64.58% 85.27% 20.69% 4.09E−20 3.88E−18 UQCRQ ENST00000498309 20.30% 4.38% 15.92% ENST00000378670 74.04% 92.34% 18.30% 7.54E−20 6.98E−18 ENTPD1 ENST00000371205 86.08% 61.58% 24.50% ENST00000371206 11.18% 28.37% 17.19% 1.06E−19 9.54E−18 SYNE2 ENST00000358025 22.59% 11.27% 11.31% ENST00000557005 4.18% 12.25% 8.07% 1.57E−19 1.39E−17 PDLIM3 ENST00000514142 55.52% 44.17% 11.35% ENST00000284771 6.97% 40.35% 33.38% 3.44E−19 2.97E−17 PDE10A ENST00000648884 73.58% 20.23% 53.34% ENST00000649761 6.89% 26.67% 19.77% 7.65E−19 6.32E−17 MICOS10 ENST00000486890 7.80% 1.88% 5.92% ENST00000322753 55.53% 81.25% 25.72% 1.30E−18 1.05E−16 SNX10 ENST00000396376 52.54% 19.93% 32.62% ENST00000338523 16.25% 63.65% 47.39% 6.36E−18 5.04E−16 SOX6 ENST00000533411 13.96% 5.62% 8.34% ENST00000525259 11.36% 51.69% 40.33% 6.52E−18 5.07E−16 TRIM25 ENST00000316881 91.61% 81.80% 9.82% ENST00000648772 0.96% 11.43% 10.47% 8.74E−17 6.54E−15 PIK3R1 ENST00000320694 25.03% 10.03% 15.00% ENST00000521381 48.58% 59.66% 11.08% 1.42E−16 1.04E−14 CYTOR ENST00000645257 11.58% 5.08% 6.49% ENST00000642451 18.16% 30.79% 12.63% 2.84E−16 2.04E−14 UBE2D2 ENST00000511725 82.22% 42.86% 39.36% ENST00000398733 12.67% 38.96% 26.29% 7.09E−16 5.01E−14 MXD3 ENST00000503473 38.99% 13.93% 25.06% ENST00000427908 53.99% 81.58% 27.59% 1.15E−15 8.02E−14 EPC1 ENST00000495790 37.86% 24.12% 13.74% ENST00000375110 33.38% 61.22% 27.84% 1.21E−15 8.25E−14 ATP5MC1 ENST00000513347 22.23% 4.93% 17.31% ENST00000355938 45.24% 56.29% 11.05% 1.25E−15 8.36E−14 RPS25 ENST00000527853 64.66% 27.35% 37.31% ENST00000527673 33.71% 71.83% 38.11% 2.04E−15 1.32E−13 FAM13A ENST00000395002 44.13% 18.11% 26.02% ENST00000503556 6.20% 19.79% 13.58% 3.05E−15 1.95E−13 LINC01320 ENST00000659537 31.16% 16.13% 15.03% ENST00000621006 21.81% 40.32% 18.51% 5.16E−15 3.23E−13 HAVCR2 ENST00000524219 52.86% 12.55% 40.30% ENST00000522902 20.00% 39.96% 19.96% 6.49E−15 4.00E−13 TUBB6 ENST00000591909 80.66% 46.43% 34.24% ENST00000587204 2.11% 23.21% 21.10% 6.58E−15 4.00E−13 UGT1A4 ENST00000450233 31.59% 11.44% 20.15% ENST00000373409 68.41% 88.56% 20.15% 1.30E−14 7.80E−13 PIGX ENST00000392391 76.34% 28.07% 48.27% ENST00000426755 11.83% 66.67% 54.84% 1.78E−14 1.03E−12 RTTN ENST00000640769 12.64% 1.53% 11.11% ENST00000640736 2.10% 13.91% 11.81% 3.13E−14 1.79E−12 MBNL1 ENST00000466565 12.74% 5.15% 7.59% ENST00000461436 41.22% 51.37% 10.15% 1.45E−13 8.17E−12 OBSL1 ENST00000373873 56.08% 35.01% 21.07% ENST00000404537 3.84% 24.27% 20.43% 1.61E−13 8.93E−12 MTREX ENST00000518955 22.66% 6.33% 16.33% ENST00000230640 38.32% 69.06% 30.74% 1.80E−13 9.84E−12 MID1 ENST00000380780 83.27% 62.34% 20.93% ENST00000380779 1.42% 11.24% 9.82% 2.58E−13 1.39E−11 MRPS21 ENST00000581066 77.19% 51.06% 26.12% ENST00000614145 22.81% 48.94% 26.12% 2.87E−13 1.52E−11 IFI30 ENST00000407280 94.80% 74.50% 20.30% ENST00000600463 5.20% 25.50% 20.30% 2.90E−13 1.52E−11 NHSL1 ENST00000426841 32.87% 15.65% 17.22% ENST00000342260 1.36% 45.58% 44.22% 4.17E−13 2.16E−11 NPIPA5 ENST00000360151 94.16% 75.21% 18.96% ENST00000534094 5.68% 24.79% 19.11% 4.54E−13 2.32E−11 POU2AF1 ENST00000529065 27.08% 0.00% 27.08% ENST00000393067 49.05% 75.00% 25.95% 6.53E−13 3.27E−11 TMEM258 ENST00000543510 28.33% 12.11% 16.21% ENST00000537328 54.95% 80.48% 25.53% 6.56E−13 3.27E−11 ZNF407 ENST00000581829 51.35% 23.88% 27.47% ENST00000582337 11.25% 40.30% 29.05% 9.26E−13 4.56E−11 ITGA1 ENST00000506275 17.79% 10.89% 6.90% ENST00000650673 20.53% 40.23% 19.70% 1.12E−12 5.44E−11 ST6GAL1 ENST00000485105 13.80% 8.66% 5.14% ENST00000448408 63.04% 81.29% 18.24% 1.14E−12 5.48E−11 ATP5MPL ENST00000414262 34.75% 15.42% 19.34% ENST00000286953 44.76% 77.09% 32.33% 1.24E−12 5.89E−11 TM2D3 ENST00000561373 23.73% 12.31% 11.42% ENST00000560390 1.89% 21.54% 19.65% 1.42E−12 6.58E−11 ELMO1 ENST00000487336 27.01% 16.89% 10.13% ENST00000463390 4.90% 22.87% 17.98% 1.42E−12 6.58E−11 WWP2 ENST00000568818 42.95% 13.21% 29.74% ENST00000569174 6.85% 34.59% 27.74% 2.12E−12 9.56E−11 TMEM176 ENST00000492607 50.04% 19.61% 30.43% ENST00000326442 42.15% 61.03% 18.88% 3.01E−12 1.34E−10 B ATP5PD ENST00000580649 19.35% 5.05% 14.30% ENST00000301587 79.70% 94.76% 15.06% 4.35E−12 1.92E−10 APOLD1 ENST00000534843 94.87% 66.18% 28.70% ENST00000356591 4.10% 31.27% 27.17% 4.46E−12 1.95E−10 HOXA6 ENST00000521478 85.55% 36.59% 48.96% ENST00000222728 14.45% 63.41% 48.96% 4.71E−12 1.97E−10 NR4A2 ENST00000409572 84.33% 38.64% 45.69% ENST00000339562 10.63% 58.09% 47.47% 4.72E−12 1.97E−10 NPIPA9 ENST00000545114 24.74% 8.38% 16.35% ENST00000546267 18.57% 36.83% 18.26% 6.98E−12 2.86E−10 PLXNC1 ENST00000549217 33.43% 6.82% 26.61% ENST00000549810 2.37% 17.72% 15.35% 8.76E−12 3.55E−10 RTN4 ENST00000405240 21.76% 12.23% 9.53% ENST00000317610 23.27% 52.89% 29.62% 1.07E−11 4.29E−10 C12orf60 ENST00000330828 17.22% 1.01% 16.22% ENST00000543822 79.43% 98.99% 19.57% 1.15E−11 4.54E−10 ZBTB38 ENST00000509842 28.12% 3.58% 24.54% ENST00000514251 22.35% 36.04% 13.69% 1.20E−11 4.72E−10 RPL36 ENST00000347512 73.98% 62.14% 11.83% ENST00000394580 17.11% 34.06% 16.95% 1.76E−11 6.82E−10 BRD7 ENST00000401491 23.28% 1.76% 21.51% ENST00000394688 56.26% 94.12% 37.85% 1.83E−11 7.04E−10 IFITM2 ENST00000527146 5.45% 2.11% 3.34% ENST00000399815 72.45% 83.25% 10.79% 2.21E−11 8.43E−10 PDCD4 ENST00000393104 36.36% 9.42% 26.94% ENST00000489049 19.07% 38.12% 19.05% 3.17E−11 1.20E−09 Clorf122 ENST00000373043 79.39% 53.35% 26.03% ENST00000373042 12.25% 27.74% 15.50% 3.22E−11 1.20E−09 ATP5IF1 ENST00000468425 20.65% 9.69% 10.96% ENST00000335514 56.12% 73.85% 17.73% 3.33E−11 1.23E−09 CIB1 ENST00000650306 10.03% 2.05% 7.97% ENST00000328649 80.10% 94.12% 14.02% 4.11E−11 1.51E−09 NF1 ENST00000358273 11.25% 4.91% 6.34% ENST00000581113 6.55% 23.05% 16.50% 4.53E−11 1.64E−09 FNDC3A ENST00000398316 48.47% 25.51% 22.96% ENST00000378383 16.38% 27.87% 11.48% 6.05E−11 2.16E−09 FRMD4B ENST00000459638 47.61% 18.76% 28.85% ENST00000460709 1.47% 10.99% 9.51% 6.48E−11 2.29E−09 PSMA1 ENST00000527632 28.57% 12.66% 15.91% ENST00000528018 7.64% 49.37% 41.73% 7.33E−11 2.54E−09 HPS3 ENST00000296051 77.53% 61.32% 16.22% ENST00000462030 12.00% 31.94% 19.94% 7.56E−11 2.60E−09 EGLN3 ENST00000547327 13.44% 6.40% 7.04% ENST00000250457 67.27% 83.45% 16.18% 7.96E−11 2.72E−09 SCAND1 ENST00000615116 59.26% 17.24% 42.02% ENST00000373991 36.57% 75.54% 38.97% 1.08E−10 3.67E−09 NDUFB4 ENST00000485064 16.07% 5.09% 10.98% ENST00000184266 80.87% 93.72% 12.85% 1.98E−10 6.64E−09 MYSM1 ENST00000493821 24.59% 6.00% 18.59% ENST00000481973 2.48% 16.13% 13.65% 2.32E−10 7.72E−09 RBIS ENST00000611854 22.62% 11.41% 11.21% ENST00000619594 33.59% 67.93% 34.35% 2.57E−10 8.45E−09 DPP4 ENST00000461836 19.66% 2.84% 16.82% ENST00000360534 47.27% 80.85% 33.59% 2.80E−10 9.14E−09 CISD1 ENST00000489785 14.11% 1.63% 12.48% ENST00000333926 79.60% 96.16% 16.56% 3.02E−10 9.78E−09 TBCA ENST00000522370 6.97% 0.36% 6.61% ENST00000380377 67.00% 90.15% 23.15% 3.44E−10 1.11E−08 HIF1A ENST00000539097 10.85% 4.60% 6.26% ENST00000337138 24.22% 38.16% 13.94% 4.75E−10 1.51E−08 PHACTR1 ENST00000332995 57.59% 36.39% 21.21% ENST00000482982 1.25% 17.78% 16.53% 6.96E−10 2.20E−08 B2M ENST00000559220 15.54% 11.85% 3.69% ENST00000632133 52.61% 63.69% 11.08% 8.89E−10 2.79E−08 PACSIN2 ENST00000407585 31.89% 12.65% 19.25% ENST00000263246 21.95% 38.16% 16.21% 9.37E−10 2.91E−08 HOXA-AS3 ENST00000524304 81.23% 37.21% 44.02% ENST00000518947 18.09% 62.79% 44.70% 1.04E−10 3.20E−08 FKBP2 ENST00000541388 27.50% 8.56% 18.94% ENST00000309366 48.21% 74.90% 26.69% 1.56E−09 4.78E−08 IVNS1ABP ENST00000480769 56.71% 42.79% 13.92% ENST00000367498 11.22% 25.10% 13.88% 1.81E−09 5.51E−08 BOLA2 ENST00000380596 55.53% 40.22% 15.32% ENST00000330978 44.47% 59.78% 15.32% 2.63E−09 7.87E−08 NNMT ENST00000535401 77.78% 47.41% 30.37% ENST00000542647 4.08% 20.52% 16.44% 3.16E−09 9.37E−08 PDIA5 ENST00000467157 54.53% 33.70% 20.83% ENST00000469649 17.64% 53.78% 36.14% 3.21E−09 9.47E−08 CCL4 ENST00000615863 72.64% 47.99% 24.65% ENST00000613947 24.52% 48.43% 23.92% 5.08E−09 1.48E−07 CARMIL1 ENST00000329474 69.24% 38.69% 30.55% ENST00000461945 23.25% 59.61% 36.36% 5.13E−09 1.49E−07 PRSS23 ENST00000532572 44.41% 17.89% 26.51% ENST00000280258 30.59% 68.41% 37.82% 5.35E−09 1.52E−07 SOD2 ENST00000538183 44.48% 28.96% 15.51% ENST00000546260 13.46% 18.83% 5.36% 7.52E−09 2.07E−07 COA4 ENST00000544575 32.53% 15.15% 17.38% ENST00000355693 49.35% 76.26% 26.91% 7.96E−09 2.18E−07 SMARCB1 ENST00000646911 21.77% 4.74% 17.03% ENST00000644036 60.20% 91.16% 30.96% 8.04E−09 2.19E−07 PSMG2 ENST00000400514 64.21% 21.31% 42.90% ENST00000317615 20.15% 62.29% 42.14% 9.83E−09 2.64E−07 GOLGA8B ENST00000569100 22.95% 11.13% 11.81% ENST00000564575 18.28% 32.88% 14.60% 2.22E−08 5.79E−07 AC009133.1 ENST00000564980 34.34% 15.51% 18.84% ENST00000569981 45.77% 68.64% 22.87% 2.27E−08 5.87E−07 TNFSF10 ENST00000472804 28.81% 12.41% 16.40% ENST00000241261 38.91% 60.26% 21.35% 2.52E−08 6.50E−07 CBWD1 ENST00000618061 20.28% 12.19% 8.08% ENST00000382393 36.66% 51.33% 14.67% 2.70E−08 6.90E−07 PLPP3 ENST00000472957 14.83% 2.41% 12.42% ENST00000459962 14.66% 26.27% 11.60% 3.70E−08 9.33E−07 CSAD ENST00000267085 69.61% 41.90% 27.70% ENST00000424990 8.64% 23.42% 14.78% 4.06E−08 1.02E−06 ARHGAP22 ENST00000460425 63.66% 42.68% 20.97% ENST00000493012 12.16% 23.17% 11.01% 5.07E−08 1.26E−06 RHOF ENST00000546227 7.39% 0.00% 7.39% ENST00000267205 6.21% 23.14% 16.93% 5.16E−08 1.28E−06 FXYD2 ENST00000317594 20.84% 1.60% 19.23% ENST00000292079 71.95% 94.65% 22.71% 5.31E−08 1.31E−06 C4orf19 ENST00000381980 75.95% 29.03% 46.92% ENST00000508175 21.00% 54.84% 33.84% 5.78E−08 1.41E−06 PSMA2 ENST00000411875 29.65% 8.49% 21.16% ENST00000223321 63.60% 87.26% 23.66% 5.92E−08 1.43E−06 ARHGAP29 ENST00000482481 44.94% 33.30% 11.64% ENST00000260526 28.18% 48.72% 20.54% 6.19E−08 1.48E−06 HSBP1L1 ENST00000587347 16.27% 2.90% 13.37% ENST00000451882 31.67% 71.01% 39.35% 7.11E−08 1.68E−06 DNAAF1 ENST00000564928 74.50% 35.37% 39.13% ENST00000623406 14.34% 49.90% 35.56% 7.60E−08 1.79E−06 CBWD2 ENST00000479583 14.78% 10.87% 3.91% ENST00000490323 38.90% 50.07% 11.17% 1.04E−08 2.42E−06 HLA-DPA1 ENST00000419277 71.43% 61.35% 10.08% ENST00000417724 13.93% 26.62% 12.68% 1.04E−07 2.43E−06 SRPRA ENST00000530680 42.03% 14.81% 27.21% ENST00000332118 35.94% 70.37% 34.43% 1.14E−07 2.63E−06 NBPF10 ENST00000583866 49.25% 33.40% 15.85% ENST00000612520 45.82% 62.90% 17.08% 1.19E−07 2.72E−06 ZBTB25 ENST00000608382 77.74% 56.00% 21.74% ENST00000555220 9.59% 29.60% 20.01% 1.25E−07 2.84E−06 PCGF3 ENST00000362003 85.93% 72.99% 12.94% ENST00000400151 0.34% 7.51% 7.17% 1.48E−07 3.35E−06 DICER1 ENST00000526495 61.93% 27.52% 34.41% ENST00000527414 7.18% 15.57% 8.39% 1.73E−07 3.86E−06 DNM2 ENST00000590806 10.69% 5.34% 5.35% ENST00000593203 34.70% 67.09% 32.38% 2.23E−07 4.92E−06 RNPC3 ENST00000531883 44.89% 39.08% 5.81% ENST00000533834 10.23% 23.30% 13.07% 2.43E−07 5.31E−06 MAGI1 ENST00000464060 48.76% 34.84% 13.92% ENST00000330909 16.71% 27.69% 10.98% 2.59E−07 5.56E−06 SNX29 ENST00000566228 59.28% 42.35% 16.93% ENST00000564791 17.07% 26.17% 9.10% 2.78E−07 5.93E−06 ERCC5 ENST00000602836 64.85% 26.67% 38.18% ENST00000651281 12.42% 26.67% 14.24% 3.29E−07 7.00E−06 VDAC1 ENST00000265333 22.29% 8.96% 13.32% ENST00000395044 58.30% 82.54% 24.25% 3.42E−07 7.22E−06 RPL7 ENST00000396467 47.34% 23.81% 23.53% ENST00000352983 41.18% 74.60% 33.42% 3.45E−07 7.24E−06 TPM1 ENST00000560131 18.00% 4.26% 13.74% ENST00000560679 6.46% 17.54% 11.08% 3.48E−07 7.27E−06 COX16 ENST00000555276 76.78% 58.70% 18.08% ENST00000389912 16.10% 29.16% 13.06% 3.60E−07 7.48E−06 TFRC ENST00000360110 32.59% 23.49% 9.10% ENST00000392396 14.21% 28.56% 14.36% 4.06E−07 8.40E−06 MRTFB ENST00000572400 41.28% 18.82% 22.47% ENST00000572567 11.49% 36.28% 24.79% 6.09E−07 1.25E−05 CACNB2 ENST00000324631 78.25% 35.84% 42.41% ENST00000652391 2.91% 43.34% 40.42% 7.04E−07 1.42E−05 BOLA2B ENST00000567436 22.44% 10.19% 12.25% ENST00000305321 65.00% 86.15% 21.15% 7.19E−07 1.44E−05 POLRIC ENST00000646700 47.50% 23.29% 24.21% ENST00000646188 21.25% 67.12% 45.87% 8.53E−07 1.70E−05 AC068587.4 ENST00000641618 55.72% 13.11% 42.61% ENST00000635775 12.21% 29.51% 17.29% 8.86E−07 1.74E−05 KAT8 ENST00000539683 42.98% 29.63% 13.35% ENST00000543774 2.58% 28.14% 25.56% 9.10E−07 1.78E−05 SLC9A3R1 ENST00000578958 33.83% 15.60% 18.23% ENST00000262613 65.25% 83.89% 18.64% 9.52E−07 1.85E−05 PILRB ENST00000452089 17.55% 4.82% 12.73% ENST00000493091 66.40% 89.32% 22.92% 9.72E−07 1.88E−05 ATIC ENST00000467388 46.54% 31.17% 15.38% ENST00000236959 9.13% 41.56% 32.42% 1.12E−06 2.15E−05 FILIP1L ENST00000398326 42.48% 8.06% 34.41% ENST00000383694 18.62% 57.51% 38.89% 1.21E−06 2.30E−05 NBPF15 ENST00000577412 20.83% 8.59% 12.24% ENST00000584793 56.76% 76.41% 19.65% 1.24E−06 2.35E−05 ZNF710- ENST00000620791 67.17% 39.25% 27.92% ENST00000558334 32.83% 60.75% 27.92% 1.29E−06 2.43E−05 AS1 SELENOS ENST00000534014 55.94% 36.48% 19.45% ENST00000526049 21.20% 33.79% 12.58% 1.30E−06 2.44E−05 AC106791.1 ENST00000661977 59.61% 16.67% 42.94% ENST00000670115 11.17% 28.02% 16.85% 1.36E−06 2.54E−05 CCNH ENST00000505587 47.67% 30.02% 17.65% ENST00000504878 7.70% 24.12% 16.43% 1.40E−06 2.60E−05 PAPPA2 ENST00000367661 31.41% 17.74% 13.67% ENST00000367662 61.88% 77.93% 16.05% 1.45E−06 2.69E−05 7-Mar ENST00000259050 40.45% 15.34% 25.11% ENST00000473749 18.53% 39.58% 21.05% 1.49E−06 2.75E−05 MRPS34 ENST00000569585 23.30% 7.91% 15.39% ENST00000397375 76.70% 92.09% 15.39% 1.59E−06 2.92E−05 MTUS1 ENST00000517413 23.84% 12.68% 11.17% ENST00000297488 6.89% 21.25% 14.36% 1.68E−06 3.06E−05 SEC24D ENST00000505134 21.95% 10.86% 11.09% ENST00000511033 4.74% 11.56% 6.82% 1.91E−06 3.46E−05 HSPD1 ENST00000476746 12.89% 4.10% 8.79% ENST00000388968 22.69% 34.60% 11.91% 1.96E−06 3.52E−05 GNPTAB ENST00000299314 75.82% 63.28% 12.54% ENST00000549165 6.79% 15.28% 8.49% 2.16E−06 3.86E−05 SNRNP35 ENST00000527158 66.06% 39.29% 26.77% ENST00000526639 13.36% 35.66% 22.30% 2.21E−06 3.94E−05 PCSK6 ENST00000611967 24.64% 16.94% 7.70% ENST00000558154 7.14% 23.11% 15.98% 2.64E−06 4.65E−05 Z93930.2 ENST00000458080 22.10% 5.00% 17.10% ENST00000585003 65.17% 86.00% 20.83% 2.95E−06 5.17E−05 POM121C ENST00000615331 42.24% 8.10% 34.14% ENST00000607367 9.84% 30.64% 20.80% 3.24E−06 5.65E−05 ERP29 ENST00000553161 15.77% 5.58% 10.19% ENST00000261735 62.03% 81.96% 19.93% 4.20E−06 7.15E−05 ELF1 ENST00000405737 36.94% 21.20% 15.74% ENST00000635415 4.05% 20.40% 16.34% 4.34E−06 7.36E−05 PPP1R14B ENST00000542235 23.64% 6.06% 17.58% ENST00000309318 59.18% 70.30% 11.13% 5.48E−06 9.18E−05 NDUFB1 ENST00000553666 27.53% 17.41% 10.12% ENST00000553514 4.86% 21.87% 17.00% 5.69E−06 9.50E−05 AFF1 ENST00000307808 31.51% 19.61% 11.90% ENST00000511442 8.44% 24.93% 16.49% 7.10E−06 0.00012 NGLY1 ENST00000417874 47.35% 21.22% 26.14% ENST00000496726 13.36% 32.83% 19.48% 7.44E−06 0.00012 NIPSNAP2 ENST00000497279 54.56% 37.29% 17.26% ENST00000446692 20.44% 49.46% 29.02% 8.02E−06 0.00013 EIF3K ENST00000590134 30.19% 11.80% 18.39% ENST00000586932 3.79% 17.31% 13.52% 8.80E−06 0.00014 CTAG2 ENST00000247306 60.17% 30.86% 29.30% ENST00000369585 39.83% 69.14% 29.30% 8.93E−06 0.00014 AL354740.1 ENST00000429998 55.25% 35.98% 19.27% ENST00000593917 40.68% 60.59% 19.92% 9.48E−06 0.00015 AC004889.1 ENST00000663681 66.12% 47.17% 18.95% ENST00000498693 0.84% 6.60% 5.77% 1.03E−05 0.00016 EIF3B ENST00000468611 41.93% 34.28% 7.64% ENST00000397011 5.35% 20.91% 15.55% 1.03E−05 0.00016 DTYMK ENST00000445261 84.34% 63.83% 20.51% ENST00000420144 1.24% 17.02% 15.78% 1.25E−05 0.0002 PLD1 ENST00000471075 23.32% 10.28% 13.04% ENST00000331659 26.92% 35.80% 8.88% 1.31E−05 0.0002 Clorf52 ENST00000294661 88.84% 60.14% 28.70% ENST00000471115 6.12% 27.14% 21.02% 1.33E−05 0.00021 CHCHD1 ENST00000372837 34.22% 14.88% 19.34% ENST00000372833 65.78% 85.12% 19.34% 1.59E−05 0.00025 TNRC6B ENST00000454349 23.84% 11.83% 12.01% ENST00000301923 23.63% 47.15% 23.52% 1.62E−05 0.00025 CEP95 ENST00000577960 12.39% 1.41% 10.98% ENST00000583676 14.02% 36.62% 22.60% 1.62E−05 0.00025 C5AR1 ENST00000595501 21.50% 4.88% 16.62% ENST00000355085 52.47% 70.73% 18.27% 1.77E−05 0.00027 AC138969.1 ENST00000381497 21.61% 3.66% 17.95% ENST00000534258 12.36% 18.63% 6.27% 1.81E−05 0.00027 SEC11C ENST00000585864 6.20% 0.40% 5.79% ENST00000587834 69.66% 85.22% 15.56% 1.91E−05 0.00029 SNHG9 ENST00000531523 91.40% 78.62% 12.78% ENST00000564014 8.60% 21.38% 12.78% 1.97E−05 0.0003 WARS2- ENST00000425884 14.67% 1.85% 12.81% ENST00000670000 20.00% 31.48% 11.48% 1.99E−05 0.0003 AS1 AC010618.3 ENST00000655024 12.35% 3.41% 8.94% ENST00000596643 61.27% 79.55% 18.28% 2.07E−05 0.00031 NBPF14 ENST00000619423 21.84% 8.76% 13.08% ENST00000606877 58.30% 72.65% 14.35% 2.15E−05 0.00032 UPF3A ENST00000492270 28.32% 14.78% 13.54% ENST00000480362 0.19% 2.74% 2.56% 2.16E−05 0.00032 IMMP1L ENST00000528161 11.59% 4.26% 7.33% ENST00000526776 0.58% 14.89% 14.32% 2.45E−05 0.00036 MTPAP ENST00000263063 86.87% 58.18% 28.69% ENST00000421701 12.83% 37.74% 24.91% 2.47E−05 0.00036 CDH6 ENST00000508132 22.12% 0.00% 22.12% ENST00000506396 3.81% 21.79% 17.98% 2.52E−05 0.00037 USP22 ENST00000261497 17.79% 2.95% 14.84% ENST00000478443 36.24% 66.87% 30.63% 2.55E−05 0.00037 ARID5B ENST00000644638 23.95% 10.56% 13.39% ENST00000309334 31.22% 49.03% 17.82% 2.61E−05 0.00038 TMC3-AS1 ENST00000664001 31.51% 10.00% 21.51% ENST00000667513 16.70% 44.00% 27.30% 2.68E−05 0.00039 AIG1 ENST00000646199 44.88% 29.13% 15.75% ENST00000367596 5.78% 30.43% 24.65% 3.04E−05 0.00044 FAAP20 ENST00000497675 21.53% 10.37% 11.17% ENST00000378546 31.22% 41.41% 10.20% 3.09E−05 0.00044 ZNF468 ENST00000391781 90.56% 72.41% 18.14% ENST00000602144 1.34% 10.34% 9.00% 3.15E−05 0.00045 PSMB10 ENST00000575556 14.18% 0.81% 13.37% ENST00000358514 75.30% 87.90% 12.60% 3.14E−05 0.00045 ANPEP ENST00000559761 15.26% 3.09% 12.17% ENST00000300060 67.70% 87.71% 20.00% 3.16E−05 0.00045 CKMT2- ENST00000500148 31.63% 7.41% 24.22% ENST00000511495 3.29% 27.91% 24.62% 3.22E−05 0.00045 AS1 UGT2B7 ENST00000502942 88.34% 74.60% 13.74% ENST00000305231 7.63% 25.40% 17.77% 3.26E−05 0.00046 ZBTB1 ENST00000554015 62.87% 37.96% 24.91% ENST00000358738 34.59% 57.41% 22.82% 3.58E−05 0.0005 TMEM91 ENST00000342187 51.62% 19.51% 32.11% ENST00000413014 44.62% 79.27% 34.64% 3.73E−05 0.00052 SIPA1L3 ENST00000601054 14.09% 0.79% 13.30% ENST00000222345 59.48% 72.22% 12.74% 3.86E−05 0.00053 SNHG8 ENST00000602520 68.11% 57.65% 10.46% ENST00000602819 7.93% 27.06% 19.13% 3.88E−05 0.00053 TCEAL9 ENST00000646896 36.89% 24.18% 12.72% ENST00000372661 50.29% 74.72% 24.44% 4.58E−05 0.00062 RGPD8 ENST00000302558 96.11% 83.83% 12.29% ENST00000522286 0.83% 8.41% 7.58% 4.58E−05 0.00062 LINC-PINT ENST00000451786 22.79% 13.72% 9.07% ENST00000429901 14.94% 25.01% 10.07% 4.58E−05 0.00062 CDK6 ENST00000265734 94.01% 84.13% 9.88% ENST00000424848 4.03% 15.87% 11.85% 4.90E−05 0.00066 CNN3 ENST00000474409 27.84% 16.79% 11.04% ENST00000370206 55.14% 78.95% 23.81% 5.08E−05 0.00068 ATXN2 ENST00000482777 15.16% 5.99% 9.17% ENST00000642389 7.31% 24.78% 17.47% 5.10E−05 0.00068 MAST4 ENST00000405643 30.75% 12.99% 17.76% ENST00000403625 30.06% 39.17% 9.10% 5.18E−05 0.00069 NRP1 ENST00000455749 19.53% 8.64% 10.89% ENST00000374867 2.50% 8.42% 5.92% 5.29E−05 0.0007 GSTM4 ENST00000326729 33.40% 8.68% 24.71% ENST00000638994 56.11% 86.05% 29.94% 6.15E−05 0.00081 CREBBP ENST00000573672 11.91% 4.90% 7.01% ENST00000635753 4.84% 18.18% 13.34% 6.65E−05 0.00087 CCDC191 ENST00000295878 51.27% 33.01% 18.25% ENST00000481358 14.25% 40.00% 25.75% 6.74E−05 0.00088 SLC25A13 ENST00000416240 35.90% 21.68% 14.22% ENST00000472162 15.61% 39.42% 23.82% 6.72E−05 0.00088 TTC3 ENST00000399017 63.38% 49.51% 13.87% ENST00000399010 8.99% 15.51% 6.53% 6.71E−05 0.00088 CHFR ENST00000536932 50.53% 21.04% 29.49% ENST00000266880 23.11% 32.76% 9.65% 6.83E−05 0.00089 HSPE1 ENST00000473395 19.46% 8.19% 11.27% ENST00000233893 71.21% 90.96% 19.75% 7.46E−05 0.00096 SSU72 ENST00000359060 82.26% 60.71% 21.55% ENST00000291386 12.24% 34.90% 22.66% 8.13E−05 0.00104 CCDC58 ENST00000479899 26.17% 8.82% 17.35% ENST00000291458 19.25% 61.76% 42.52% 8.12E−05 0.00104 FAM204A ENST00000369183 75.00% 59.42% 15.58% ENST00000369172 0.38% 11.59% 11.21% 8.46E−05 0.00107 NR2F2 ENST00000421109 55.86% 30.23% 25.63% ENST00000394166 31.81% 69.75% 37.93% 8.59E−05 0.00109 AC008443.1 ENST00000599439 81.79% 65.52% 16.27% ENST00000511331 3.79% 17.24% 13.45% 8.71E−05 0.0011 TIMM17A ENST00000484647 22.35% 6.41% 15.93% ENST00000367287 49.86% 80.77% 30.91% 8.72E−05 0.0011 PRKCE ENST00000497602 19.68% 11.11% 8.57% ENST00000480633 2.02% 14.45% 12.43% 8.86E−05 0.00111 ANKRD28 ENST00000498524 10.19% 4.89% 5.30% ENST00000461696 56.17% 73.40% 17.22% 9.07E−05 0.00113 INPP4B ENST00000513000 53.11% 43.62% 9.50% ENST00000511838 3.69% 21.26% 17.57% 9.27E−05 0.00115 ZNF783 ENST00000378052 38.59% 12.20% 26.39% ENST00000434415 39.30% 80.49% 41.19% 0.0001 0.00124 SENP5 ENST00000323460 87.59% 64.58% 23.01% ENST00000463245 2.76% 17.19% 14.43% 0.000101 0.00125 VAV3 ENST00000479977 20.08% 3.64% 16.43% ENST00000343258 1.00% 13.64% 12.63% 0.000107 0.00131 AC092821.3 ENST00000641608 42.05% 17.00% 25.05% ENST00000642112 12.01% 31.97% 19.96% 0.000107 0.00131 FAM72A ENST00000367128 29.91% 3.92% 25.99% ENST00000468509 53.75% 83.91% 30.16% 0.000111 0.00135 SERF2 ENST00000448830 39.03% 28.42% 10.61% ENST00000381359 1.42% 6.52% 5.10% 0.00012 0.00145 ABCB9 ENST00000541424 7.99% 1.06% 6.93% ENST00000542678 86.98% 97.24% 10.26% 0.000121 0.00145 HSPB11 ENST00000489675 16.67% 5.10% 11.57% ENST00000194214 49.55% 75.51% 25.96% 0.000122 0.00146 SIVA1 ENST00000556431 43.84% 21.05% 22.79% ENST00000329967 52.92% 75.44% 22.52% 0.000127 0.00152 TAOK3 ENST00000537952 27.89% 16.80% 11.09% ENST00000542902 0.70% 8.55% 7.85% 0.000131 0.00157 TRIR ENST00000589590 14.80% 3.91% 10.89% ENST00000588213 32.14% 43.02% 10.88% 0.000133 0.00158 MIA3 ENST00000470521 21.58% 13.88% 7.70% ENST00000450260 1.59% 16.85% 15.27% 0.00013 0.00161 LAMA1 ENST00000389658 48.97% 14.69% 34.27% ENST00000490190 8.15% 20.83% 12.68% 0.000138 0.00163 KIFC1 ENST00000428849 38.85% 11.11% 27.74% ENST00000486695 56.45% 88.89% 32.44% 0.000142 0.0016 DUSP23 ENST00000368107 55.88% 29.48% 26.41% ENST00000368108 44.12% 70.52% 26.41% 0.000148 0.00173 GSTK1 ENST00000479303 52.71% 46.29% 6.42% ENST00000358406 30.20% 45.12% 14.92% 0.00015 0.00175 AC242426.2 ENST00000650785 77.11% 62.40% 14.71% ENST00000651151 21.42% 32.00% 10.58% 0.000166 0.00191 ARAP1 ENST00000359373 23.89% 3.18% 20.71% ENST00000465814 34.96% 65.35% 30.40% 0.00017 0.00196 AC093535.1 ENST00000648070 47.17% 17.95% 29.22% ENST00000512500 44.11% 56.41% 12.30% 0.000171 0.00196 COX20 ENST00000411948 9.78% 4.38% 5.40% ENST00000498262 33.59% 44.64% 11.05% 0.000175 0.002 RUFY2 ENST00000473398 23.48% 8.00% 15.48% ENST00000466187 49.79% 74.48% 24.69% 0.000177 0.00201 AL137186.2 ENST00000397264 97.79% 82.61% 15.19% ENST00000448685 1.47% 17.39% 15.92% 0.0002 0.00225 URI1 ENST00000570564 39.45% 25.97% 13.47% ENST00000573052 2.08% 12.99% 10.91% 0.000219 0.00246 GSAP ENST00000491796 16.97% 8.88% 8.08% ENST00000434084 8.68% 18.71% 10.03% 0.000226 0.00253 IER3 ENST00000376377 45.85% 32.74% 13.11% ENST00000259874 54.15% 67.26% 13.11% 0.00023 0.00257 MRPL16 ENST00000525470 35.68% 9.33% 26.35% ENST00000300151 47.72% 69.33% 21.61% 0.00024 0.00268 MOB1B ENST00000309395 72.65% 60.72% 11.93% ENST00000511449 10.26% 23.68% 13.43% 0.000265 0.00293 MROH1 ENST00000423230 22.32% 11.88% 10.44% ENST00000528664 4.09% 14.85% 10.76% 0.000266 0.00293 VPS9D1 ENST00000389386 36.76% 10.00% 26.76% ENST00000561976 56.10% 88.33% 32.23% 0.000269 0.00295 ITGAE ENST00000572179 20.10% 6.88% 13.22% ENST00000263087 49.25% 68.54% 19.29% 0.000273 0.00299 RBX1 ENST00000467617 14.34% 5.38% 8.96% ENST00000216225 83.61% 94.22% 10.61% 0.000293 0.0032 NDUFAB1 ENST00000484769 7.49% 0.44% 7.05% ENST00000007516 84.10% 95.15% 11.05% 0.000296 0.00322 TULP3 ENST00000448120 71.41% 48.15% 23.26% ENST00000540184 17.84% 50.00% 32.16% 0.000303 0.00329 TASOR2 ENST00000645567 30.99% 12.76% 18.23% ENST00000482419 26.64% 44.71% 18.07% 0.000306 0.00332 GNPTG ENST00000527137 39.36% 17.81% 21.55% ENST00000204679 42.58% 72.60% 30.02% 0.00031 0.00335 C12orf45 ENST00000552951 87.43% 68.37% 19.05% ENST00000547750 3.43% 14.29% 10.86% 0.000343 0.00368 SSBP1 ENST00000489378 38.94% 24.53% 14.41% ENST00000465582 10.30% 35.85% 25.55% 0.000346 0.00369 VRK2 ENST00000432057 18.39% 2.82% 15.58% ENST00000412104 0.89% 11.27% 10.38% 0.00035 0.00372 CHSY1 ENST00000561143 13.47% 3.93% 9.54% ENST00000254190 71.24% 89.80% 18.56% 0.000356 0.00378 EMP2 ENST00000536829 19.62% 1.61% 18.00% ENST00000359543 57.31% 83.87% 26.56% 0.000358 0.0038 SSH2 ENST00000578411 23.88% 7.41% 16.48% ENST00000649863 27.30% 45.47% 18.17% 0.000367 0.00387 RNMT ENST00000592764 8.84% 1.93% 6.91% ENST00000543302 26.36% 39.17% 12.80% 0.000367 0.00387 PPFIBP2 ENST00000530181 42.31% 16.56% 25.75% ENST00000528883 8.21% 18.60% 10.40% 0.000374 0.00393 CMC4 ENST00000369479 71.05% 53.27% 17.78% ENST00000369484 28.95% 46.73% 17.78% 0.000397 0.00414 ISCA1 ENST00000326094 26.39% 3.17% 23.21% ENST00000375991 72.38% 95.24% 22.85% 0.000407 0.00423 SIAE ENST00000436137 23.08% 3.95% 19.13% ENST00000525730 16.48% 31.58% 15.10% 0.000408 0.00423 PLXDC2 ENST00000377242 26.11% 9.84% 16.27% ENST00000377252 73.20% 90.16% 16.96% 0.00041 0.00424 AC018362.1 ENST00000649053 45.77% 24.00% 21.77% ENST00000564805 54.23% 76.00% 21.77% 0.000416 0.00429 MAPILC3B2 ENST00000625301 16.54% 1.89% 14.65% ENST00000556529 83.46% 98.11% 14.65% 0.000418 0.0043 AC009060.1 ENST00000671539 32.29% 8.35% 23.94% ENST00000502126 37.36% 50.90% 13.55% 0.000433 0.00443 PTPN14 ENST00000366956 68.93% 56.10% 12.83% ENST00000491277 24.91% 41.31% 16.40% 0.000433 0.00443 RMST ENST00000667445 12.72% 3.60% 9.12% ENST00000639542 28.11% 58.31% 30.20% 0.000461 0.00469 COPG1 ENST00000504547 15.77% 2.63% 13.13% ENST00000513965 21.89% 52.63% 30.74% 0.00047 0.00476 CLEC18A ENST00000568102 69.69% 58.33% 11.36% ENST00000449317 1.42% 10.71% 9.29% 0.000499 0.00501 TPT1 ENST00000490277 20.47% 10.58% 9.88% ENST00000530705 68.96% 80.53% 11.57% 0.000506 0.00506 FRYL ENST00000358350 17.81% 7.14% 10.67% ENST00000302806 21.22% 40.72% 19.50% 0.000532 0.0053 DNAJA2 ENST00000617000 13.94% 1.10% 12.84% ENST00000317089 80.52% 98.90% 18.38% 0.000547 0.00544 MRPL33 ENST00000448427 82.54% 64.46% 18.07% ENST00000296102 6.19% 12.97% 6.77% 0.000569 0.00562 SULF2 ENST00000484875 14.94% 4.67% 10.27% ENST00000359930 17.57% 31.62% 14.05% 0.000572 0.00563 SLC40A1 ENST00000418714 32.51% 8.33% 24.18% ENST00000261024 63.02% 89.29% 26.27% 0.000592 0.0058 BICD1 ENST00000652176 57.86% 35.91% 21.95% ENST00000552160 13.37% 24.76% 11.40% 0.000611 0.00595 NPIPA1 ENST00000472413 47.46% 34.40% 13.05% ENST00000534720 8.77% 19.29% 10.51% 0.000612 0.00595 TRIB3 ENST00000615226 14.21% 4.88% 9.33% ENST00000217233 82.55% 95.12% 12.57% 0.000625 0.00605 KLHL28 ENST00000553817 60.98% 44.15% 16.83% ENST00000396128 35.69% 47.05% 11.36% 0.000633 0.00611 PTPN18 ENST00000420717 18.84% 10.28% 8.56% ENST00000175756 47.79% 71.03% 23.23% 0.000661 0.00633 TRIM14 ENST00000341469 72.69% 62.39% 10.29% ENST00000478530 15.85% 35.90% 20.04% 0.000685 0.00654 PRKG1 ENST00000643582 18.90% 9.86% 9.04% ENST00000401604 11.97% 37.61% 25.64% 0.000703 0.00668 JMJDIC ENST00000633035 32.36% 21.26% 11.10% ENST00000399262 25.65% 30.12% 4.48% 0.000714 0.00677 ANKRD12 ENST00000262126 45.04% 35.26% 9.78% ENST00000540578 19.56% 30.56% 11.00% 0.000727 0.00686 ASAP1 ENST00000357668 18.97% 8.68% 10.29% ENST00000518957 20.33% 28.99% 8.66% 0.000732 0.00687 RPS6KA3 ENST00000379565 69.78% 60.91% 8.87% ENST00000647265 5.64% 17.59% 11.95% 0.000732 0.00687 ALPK1 ENST00000509688 22.54% 9.62% 12.92% ENST00000426472 9.77% 24.74% 14.96% 0.000742 0.00695 UHRF1BP1 ENST00000192788 58.45% 36.52% 21.93% ENST00000452449 41.55% 63.48% 21.93% 0.000747 0.00697 ST5 ENST00000531237 84.05% 66.67% 17.38% ENST00000533016 1.76% 11.59% 9.83% 0.000748 0.00697 HSP90AA1 ENST00000555662 5.26% 0.00% 5.26% ENST00000216281 67.89% 81.94% 14.05% 0.000767 0.00711 PI4KA ENST00000255882 24.96% 20.10% 4.86% ENST00000449120 13.72% 30.91% 17.19% 0.000786 0.00724 SLC16A1 ENST00000478835 63.52% 51.47% 12.05% ENST00000429288 14.07% 35.29% 21.23% 0.000789 0.00725 FYB1 ENST00000515010 27.20% 13.43% 13.77% ENST00000512982 3.34% 9.35% 6.01% 0.000796 0.0073 ANKRD23 ENST00000476975 32.40% 20.80% 11.60% ENST00000462692 54.62% 75.20% 20.58% 0.000799 0.00731 FAR2 ENST00000551193 82.00% 50.79% 31.21% ENST00000536681 13.40% 22.36% 8.96% 0.000831 0.00755 POGZ ENST00000358476 9.40% 3.88% 5.52% ENST00000485040 32.33% 54.73% 22.40% 0.000858 0.00777 ING5 ENST00000313552 44.43% 32.31% 12.12% ENST00000492488 3.91% 21.54% 17.63% 0.00086 0.00778 NDUFA3 ENST00000482960 16.98% 1.89% 15.10% ENST00000485876 24.28% 62.91% 38.63% 0.000867 0.00782 LINC01151 ENST00000656927 11.05% 2.59% 8.46% ENST00000666442 22.84% 34.48% 11.64% 0.00087 0.00782 PSMC1 ENST00000555787 62.67% 40.20% 22.47% ENST00000261303 33.09% 50.19% 17.10% 0.000908 0.00811 ATXN1L ENST00000427980 72.98% 48.15% 24.84% ENST00000569119 19.35% 41.98% 22.62% 0.000946 0.00841 SPCS3 ENST00000507678 20.61% 10.59% 10.02% ENST00000503362 78.93% 87.71% 8.78% 0.000968 0.00859 PPHLN1 ENST00000549774 34.30% 23.08% 11.22% ENST00000317560 0.35% 8.65% 8.30% 0.000996 0.00878 CHD2 ENST00000394196 26.95% 16.68% 10.28% ENST00000625990 1.02% 4.15% 3.13% 0.000995 0.00878 USP15 ENST00000353364 9.31% 5.23% 4.08% ENST00000537297 17.44% 29.27% 11.83% 0.001056 0.00924 RGS12 ENST00000512266 51.51% 32.10% 19.41% ENST00000504194 0.00% 6.17% 6.17% 0.001059 0.00925 MDH1 ENST00000472098 36.59% 25.64% 10.95% ENST00000462944 17.50% 28.21% 10.70% 0.001065 0.00928 MTMR10 ENST00000568604 83.23% 57.83% 25.40% ENST00000566338 4.12% 19.28% 15.15% 0.001082 0.00936 RBM25 ENST00000261973 22.20% 12.11% 10.09% ENST00000532683 64.33% 79.21% 14.88% 0.001087 0.00937 STIM2 ENST00000478425 39.43% 16.52% 22.90% ENST00000477474 31.36% 50.87% 19.51% 0.001104 0.00947 B3GALNT2 ENST00000494378 24.92% 15.04% 9.87% ENST00000612859 8.05% 23.30% 15.25% 0.001103 0.00947 MINCR ENST00000671046 69.59% 41.67% 27.92% ENST00000518073 17.05% 35.00% 17.95% 0.00113 0.00965 AC011447.3 ENST00000585816 9.75% 4.46% 5.29% ENST00000657925 77.17% 93.58% 16.42% 0.001135 0.00968 STARD13 ENST00000487412 38.46% 17.15% 21.31% ENST00000344312 15.05% 31.97% 16.92% 0.001141 0.00971 CCDC9 ENST00000600117 51.74% 28.95% 22.79% ENST00000221922 14.27% 42.11% 27.83% 0.001152 0.00978 CCP110 ENST00000381396 29.11% 9.73% 19.38% ENST00000566523 8.88% 16.95% 8.07% 0.001178 0.00995 VTI1A ENST00000432306 35.06% 27.32% 7.74% ENST00000489142 5.41% 15.46% 10.06% 0.001227 0.01023 BX005266.2 ENST00000446626 94.44% 85.00% 9.44% ENST00000450704 2.22% 15.00% 12.78% 0.00123 0.01024 SNX24 ENST00000261369 43.50% 26.19% 17.31% ENST00000511545 5.49% 23.81% 18.32% 0.001267 0.0105 SUSD6 ENST00000342745 96.67% 85.71% 10.95% ENST00000553497 2.67% 13.84% 11.17% 0.00132 0.01087 OLA1 ENST00000497760 11.90% 0.00% 11.90% ENST00000392560 4.17% 22.22% 18.06% 0.001341 0.01102 ANKRA2 ENST00000515804 45.78% 28.05% 17.73% ENST00000296785 38.23% 51.22% 12.99% 0.001346 0.01104 MYO1B ENST00000471904 33.07% 17.14% 15.93% ENST00000304164 4.05% 12.06% 8.01% 0.001376 0.01126 EPB41 ENST00000646800 8.90% 1.45% 7.45% ENST00000648891 10.98% 26.57% 15.59% 0.001381 0.01128 LINC02802 ENST00000294715 23.25% 1.92% 21.33% ENST00000457390 50.37% 77.56% 27.19% 0.00143 0.01161 NSD3 ENST00000528828 11.65% 5.22% 6.43% ENST00000528627 10.45% 20.90% 10.44% 0.001433 0.01161 FNTB ENST00000555742 26.57% 7.89% 18.68% ENST00000557300 10.55% 36.84% 26.29% 0.001479 0.01192 UXT ENST00000376964 11.04% 2.78% 8.26% ENST00000333119 76.30% 90.74% 14.44% 0.001523 0.012265 PSMB7 ENST00000474081 25.59% 14.55% 11.05% ENST00000259457 62.47% 83.64% 21.17% 0.001541 0.0123 MCMBP ENST00000569515 55.00% 29.73% 25.27% ENST00000360003 26.26% 59.84% 33.58% 0.001556 0.01245 TMEM134 ENST00000501408 52.86% 20.93% 31.93% ENST00000308022 41.40% 76.81% 35.40% 0.001562 0.01247 MED15 ENST00000445987 38.99% 19.57% 19.43% ENST00000489651 26.15% 38.45% 12.30% 0.001595 0.01271 ZC3H8 ENST00000464305 11.07% 4.11% 6.96% ENST00000466259 4.62% 17.81% 13.19% 0.001632 0.01295 PPP1R12B ENST00000498070 10.95% 2.50% 8.45% ENST00000634903 3.36% 15.14% 11.77% 0.001692 0.01334 ASXL2 ENST00000497092 6.89% 0.00% 6.89% ENST00000673455 14.23% 27.66% 13.43% 0.001702 0.01339 NUDT8 ENST00000301490 38.91% 16.67% 22.25% ENST00000376693 40.22% 76.67% 36.45% 0.001723 0.0135 MAN2C1 ENST00000565699 19.35% 6.74% 12.61% ENST00000564785 32.90% 63.48% 30.58% 0.001728 0.01351 SCLT1 ENST00000651532 29.26% 8.30% 20.96% ENST00000506368 20.49% 35.27% 14.77% 0.001782 0.01378 SH3GLB1 ENST00000482504 21.79% 12.20% 9.59% ENST00000370558 26.54% 48.38% 21.84% 0.001783 0.01378 AC007780.1 ENST00000592030 29.58% 17.67% 11.91% ENST00000590353 69.21% 79.53% 10.32% 0.001838 0.01409 PDCD6IP ENST00000412887 12.82% 4.44% 8.38% ENST00000459659 22.68% 38.68% 16.00% 0.001854 0.01419 MPP1 ENST00000482757 8.74% 0.00% 8.74% ENST00000491955 13.73% 46.67% 32.94% 0.001861 0.01421 CTPS1 ENST00000486889 39.79% 29.45% 10.34% ENST00000649864 2.43% 18.69% 16.25% 0.001879 0.01432 SLC39A1 ENST00000356205 36.47% 13.79% 22.68% ENST00000310483 38.23% 74.14% 35.90% 0.001904 0.01446 AC022364.1 ENST00000473753 14.85% 1.23% 13.62% ENST00000617468 85.15% 98.77% 13.62% 0.001905 0.01446 LPXN ENST00000528489 84.31% 57.14% 27.17% ENST00000395074 3.92% 18.36% 14.44% 0.001992 0.01506 MTHFD1 ENST00000650853 17.81% 5.48% 12.33% ENST00000651891 11.59% 26.97% 15.38% 0.002006 0.01513 NFYC ENST00000372669 16.72% 9.09% 7.63% ENST00000440226 19.10% 31.82% 12.71% 0.002056 0.01545 TRIM59 ENST00000543469 78.57% 65.28% 13.29% ENST00000468542 0.00% 6.94% 6.94% 0.002099 0.01575 ZBTB44 ENST00000529982 7.41% 1.66% 5.75% ENST00000525623 0.00% 10.00% 10.00% 0.002113 0.01582 TRAPPC3 ENST00000462715 47.44% 28.30% 19.13% ENST00000373166 37.62% 64.15% 26.53% 0.00212 0.01584 PACRGL ENST00000503585 88.81% 66.67% 22.14% ENST00000514663 1.21% 14.28% 13.08% 0.00214 0.0159 FBXO16 ENST00000521548 94.66% 86.05% 8.62% ENST00000520481 1.62% 11.63% 10.01% 0.002162 0.01604 EHMT1 ENST00000636081 9.12% 1.06% 8.06% ENST00000495657 46.82% 63.79% 16.97% 0.002344 0.01736 TRAF6 ENST00000529150 25.42% 6.90% 18.53% ENST00000526995 73.57% 87.93% 14.36% 0.002357 0.01742 AC027097.1 ENST00000592201 87.93% 66.67% 21.26% ENST00000591854 12.07% 33.33% 21.26% 0.002373 0.0175 FAM153A ENST00000393518 15.85% 9.80% 6.05% ENST00000360669 60.12% 74.51% 14.38% 0.002458 0.01809 NSA2 ENST00000514918 14.38% 1.92% 12.46% ENST00000610426 79.79% 97.11% 17.33% 0.002573 0.01883 CEMIP2 ENST00000377055 7.51% 2.92% 4.59% ENST00000377044 16.71% 27.22% 10.52% 0.0026 0.019 APBB2 ENST00000504484 51.76% 37.16% 14.61% ENST00000502682 3.46% 11.19% 7.73% 0.002645 0.01925 RPL10A ENST00000478340 9.46% 2.20% 7.26% ENST00000467020 54.90% 67.86% 12.95% 0.002684 0.0195 LINC01252 ENST00000665563 65.25% 37.78% 27.47% ENST00000499291 34.13% 57.78% 23.65% 0.002857 0.02072 AP3S1 ENST00000515066 32.95% 15.07% 17.89% ENST00000316788 54.55% 84.93% 30.38% 0.002866 0.02074 PDCD5 ENST00000419343 11.64% 2.42% 9.21% ENST00000221784 85.84% 96.33% 10.49% 0.002889 0.02084 SDHA ENST00000264932 26.90% 7.06% 19.84% ENST00000507522 31.48% 50.49% 19.01% 0.002993 0.0214 CEBPZOS ENST00000397226 24.73% 15.85% 8.88% ENST00000392061 2.19% 12.20% 10.00% 0.003 0.0214 PDXDC1 ENST00000570001 9.74% 1.56% 8.18% ENST00000561930 8.44% 18.95% 10.51% 0.003027 0.02151 HIP1R ENST00000535831 44.83% 25.69% 19.14% ENST00000253083 38.19% 47.67% 9.48% 0.00304 0.02156 UBR3 ENST00000477461 34.44% 23.24% 11.21% ENST00000439681 18.11% 33.24% 15.12% 0.003056 0.02164 HSP90B1 ENST00000548462 12.49% 3.90% 8.59% ENST00000299767 47.93% 70.25% 22.32% 0.003096 0.02188 EML4 ENST00000318522 31.10% 20.18% 10.92% ENST00000409040 27.35% 36.92% 9.57% 0.003253 0.0229 EIF4E ENST00000450253 56.95% 39.83% 17.12% ENST00000504472 17.92% 30.51% 12.59% 0.003262 0.02293 PELI1 ENST00000358912 68.16% 61.82% 6.34% ENST00000468869 4.58% 18.12% 13.54% 0.003284 0.02304 NEDD8 ENST00000533242 24.18% 9.68% 14.50% ENST00000250495 63.19% 83.06% 19.88% 0.003362 0.0235 NUTM2B- ENST00000665716 6.64% 2.86% 3.78% ENST00000619625 26.54% 36.79% 10.25% 0.003379 0.02358 AS1 KLHDC10 ENST00000495724 11.85% 3.28% 8.57% ENST00000335420 76.94% 91.80% 14.86% 0.003431 0.02385 XPO7 ENST00000252512 56.27% 34.67% 21.61% ENST00000518808 28.85% 44.00% 15.15% 0.003491 0.02414 LINC01116 ENST00000339037 20.05% 5.45% 14.60% ENST00000295549 75.59% 89.09% 13.50% 0.003509 0.02423 SUB1 ENST00000502453 47.01% 32.95% 14.07% ENST00000265073 41.79% 52.47% 10.68% 0.003539 0.02439 SETBP1 ENST00000649279 91.96% 80.00% 11.96% ENST00000645568 3.20% 8.42% 5.22% 0.003576 0.02451 BCCIP ENST00000278100 71.21% 60.15% 11.06% ENST00000299130 11.05% 22.93% 11.89% 0.003574 0.02451 LIMS1 ENST00000544547 19.98% 14.62% 5.36% ENST00000393310 7.56% 19.01% 11.44% 0.00362 0.02478 MLLT10 ENST00000651298 13.24% 1.32% 11.93% ENST00000651382 42.33% 67.98% 25.65% 0.0037 0.02519 RBM26 ENST00000622611 12.42% 6.70% 5.72% ENST00000449987 55.81% 68.62% 12.80% 0.0037 0.02519 CDC42SE1 ENST00000439374 93.58% 78.99% 14.59% ENST00000483763 5.53% 17.48% 11.94% 0.003716 0.02525 OR2A1- ENST00000470435 44.95% 17.95% 27.01% ENST00000475089 7.54% 25.64% 18.10% 0.003741 0.02538 AS1 CREB3L2 ENST00000616381 12.78% 8.28% 4.50% ENST00000420629 6.40% 17.18% 10.79% 0.003781 0.0256 ADH5 ENST00000508511 57.74% 35.51% 22.23% ENST00000296412 32.42% 58.32% 25.90% 0.003943 0.02647 ZSCAN31 ENST00000396838 23.10% 4.94% 18.16% ENST00000476001 66.04% 75.00% 8.96% 0.003923 0.02647 BTBD2 ENST00000587825 11.59% 1.23% 10.35% ENST00000611545 62.80% 90.12% 27.32% 0.003941 0.02647 RPS29 ENST00000396020 36.05% 26.32% 9.73% ENST00000539688 18.98% 30.26% 11.29% 0.003933 0.02647 PARVB ENST00000495824 9.02% 0.00% 9.02% ENST00000402876 3.76% 16.00% 12.24% 0.003916 0.02647 TMED5 ENST00000370280 17.87% 9.85% 8.02% ENST00000370282 28.66% 47.65% 19.00% 0.004048 0.02708 CCDC47 ENST00000584112 34.51% 18.06% 16.46% ENST00000225726 37.78% 57.14% 19.36% 0.004081 0.02726 MALSU1 ENST00000476623 31.22% 13.98% 17.24% ENST00000466681 57.56% 76.34% 18.78% 0.004104 0.02731 ZNF395 ENST00000519730 15.24% 6.03% 9.21% ENST00000344423 78.08% 90.91% 12.82% 0.004171 0.02772 GADD45B ENST00000592937 62.29% 53.80% 8.50% ENST00000585359 16.92% 31.50% 14.58% 0.004201 0.02787 AP006621.1 ENST00000532946 85.71% 74.11% 11.61% ENST00000528982 14.29% 25.89% 11.61% 0.004237 0.02805 RPL35A ENST00000647248 24.63% 10.48% 14.15% ENST00000329092 32.13% 48.91% 16.78% 0.004244 0.02806 COMMD2 ENST00000490008 31.37% 9.68% 21.70% ENST00000473414 66.34% 90.32% 23.98% 0.004322 0.02852 CHCHD5 ENST00000409719 10.50% 0.99% 9.51% ENST00000324913 64.18% 78.22% 14.03% 0.004329 0.02852 LINC00174 ENST00000416366 16.73% 3.50% 13.23% ENST00000638592 27.70% 42.75% 15.05% 0.004366 0.02862 CLIC1 ENST00000375780 7.35% 0.00% 7.35% ENST00000375784 88.23% 100.0% 11.76% 0.004363 0.02862 DOP1B ENST00000399151 66.28% 46.09% 20.19% ENST00000270190 19.77% 37.39% 17.62% 0.004402 0.0287 CBLB ENST00000476370 46.30% 32.62% 13.69% ENST00000646499 17.43% 30.49% 13.06% 0.004406 0.0287 ATP8A1 ENST00000264449 23.09% 10.88% 12.20% ENST00000510289 33.10% 42.45% 9.34% 0.004408 0.0287 PAIP2 ENST00000510409 54.10% 29.01% 25.09% ENST00000265192 37.24% 64.57% 27.33% 0.004503 0.02908 FAM13B ENST00000033079 61.98% 51.85% 10.13% ENST00000420893 2.52% 8.20% 5.68% 0.004494 0.02908 SLC25A36 ENST00000648615 11.34% 6.22% 5.12% ENST00000507429 67.84% 79.63% 11.79% 0.004501 0.02908 ARMH3 ENST00000370033 79.23% 60.28% 18.95% ENST00000311122 9.97% 26.25% 16.28% 0.004518 0.02913 CCDC88C ENST00000554165 22.60% 15.61% 6.99% ENST00000553437 10.43% 21.39% 10.96% 0.004584 0.0295 HTT ENST00000510626 28.67% 13.73% 14.93% ENST00000355072 13.21% 27.30% 14.09% 0.004649 0.02982 ENY2 ENST00000517350 57.74% 40.35% 17.39% ENST00000339942 15.42% 26.48% 11.06% 0.004721 0.03019 LCOR ENST00000469510 12.54% 1.98% 10.56% ENST00000421806 39.99% 62.38% 22.38% 0.004723 0.03019 ECPAS ENST00000259335 11.38% 3.09% 8.29% ENST00000602447 12.57% 23.27% 10.70% 0.004856 0.03089 PDK3 ENST00000568479 67.29% 59.36% 7.93% ENST00000493226 16.40% 28.49% 12.09% 0.004929 0.0313 CDC42EP1 ENST00000430687 33.00% 11.27% 21.74% ENST00000249014 65.52% 85.92% 20.40% 0.004944 0.03135 SARNP ENST00000552207 34.85% 18.18% 16.67% ENST00000336133 43.12% 59.60% 16.47% 0.00513 0.03237 NQO2 ENST00000380472 12.07% 2.70% 9.36% ENST00000380455 73.26% 91.89% 18.63% 0.005283 0.03323 PDIA3 ENST00000434494 16.05% 4.27% 11.78% ENST00000300289 75.18% 90.15% 14.97% 0.005331 0.03347 PAXX ENST00000498095 84.77% 66.05% 18.72% ENST00000371620 13.41% 30.28% 16.87% 0.005596 0.03503 MTHFS ENST00000560919 29.90% 10.91% 18.99% ENST00000258874 59.84% 79.12% 19.28% 0.005625 0.03515 LINC02422 ENST00000662662 95.24% 81.48% 13.76% ENST00000535163 4.76% 18.52% 13.76% 0.005707 0.03549 RPL31 ENST00000264258 49.96% 41.27% 8.70% ENST00000441435 18.38% 34.92% 16.54% 0.00574 0.03564 TGS1 ENST00000523948 17.90% 6.67% 11.23% ENST00000260129 78.80% 93.33% 14.53% 0.00583 0.03603 CHST11 ENST00000547956 69.05% 35.90% 33.15% ENST00000549016 22.86% 46.15% 23.30% 0.005972 0.03669 CTSC ENST00000227266 44.84% 31.72% 13.12% ENST00000524463 23.35% 30.36% 7.01% 0.005975 0.03669 IFI27L2 ENST00000555558 23.86% 14.55% 9.31% ENST00000238609 66.43% 84.54% 18.12% 0.006029 0.03696 ZNF493 ENST00000392288 15.91% 7.77% 8.14% ENST00000339914 11.28% 25.08% 13.80% 0.006112 0.03737 RAB30-DT ENST00000669005 44.53% 31.71% 12.82% ENST00000656330 34.52% 47.56% 13.04% 0.006232 0.03803 DIMT1 ENST00000514605 11.83% 4.69% 7.14% ENST00000199320 37.49% 47.92% 10.42% 0.006461 0.0393 OPTN ENST00000378757 45.81% 37.52% 8.28% ENST00000487935 7.17% 18.94% 11.77% 0.006532 0.03967 NMT2 ENST00000466201 45.53% 26.67% 18.86% ENST00000378165 40.87% 71.81% 30.94% 0.006567 0.03982 PLAGL1 ENST00000392307 93.63% 81.82% 11.81% ENST00000626022 1.98% 9.09% 7.11% 0.006694 0.04053 STRAP ENST00000539887 12.31% 3.11% 9.19% ENST00000419869 55.41% 73.25% 17.84% 0.006861 0.04141 AP002807.1 ENST00000534517 90.92% 80.29% 10.63% ENST00000529934 8.78% 17.79% 9.01% 0.006888 0.04144 PSMB4 ENST00000474100 14.83% 6.41% 8.42% ENST00000290541 35.98% 54.32% 18.34% 0.006882 0.04144 ARHGEF38 ENST00000420470 22.57% 2.17% 20.40% ENST00000510406 42.01% 67.39% 25.38% 0.007111 0.04259 ZNF771 ENST00000564550 16.81% 2.44% 14.38% ENST00000566625 71.68% 97.56% 25.88% 0.007153 0.04271 SLC25A12 ENST00000472070 5.79% 1.59% 4.20% ENST00000484227 15.03% 36.19% 21.15% 0.007151 0.04271 NEDD4L ENST00000456986 7.32% 0.74% 6.58% ENST00000617539 12.68% 35.85% 23.17% 0.00718 0.0428 GORASP2 ENST00000234160 56.21% 40.00% 16.21% ENST00000486498 16.19% 26.67% 10.47% 0.007228 0.04302 PPP4R3A ENST00000554684 24.43% 14.12% 10.31% ENST00000554574 42.65% 62.08% 19.43% 0.007391 0.04375 SNRPF ENST00000551316 10.02% 2.52% 7.50% ENST00000266735 82.31% 92.45% 10.14% 0.007397 0.04375 ATP1A1- ENST00000608511 17.26% 4.88% 12.38% ENST00000493908 78.82% 95.12% 16.30% 0.007446 0.04385 AS1 ARHGEF12 ENST00000530388 17.63% 13.49% 4.15% ENST00000528225 19.50% 29.65% 10.15% 0.007437 0.04385 ATR ENST00000653868 29.94% 23.75% 6.18% ENST00000657914 9.04% 20.24% 11.20% 0.007527 0.04426 GPBP1L1 ENST00000479235 24.63% 8.90% 15.73% ENST00000496278 42.45% 56.71% 14.26% 0.007576 0.04448 UBE2G2 ENST00000345496 20.63% 6.46% 14.17% ENST00000462569 24.44% 40.21% 15.77% 0.007738 0.04523 RNF181 ENST00000443647 17.74% 9.43% 8.30% ENST00000306368 76.86% 89.62% 12.76% 0.007728 0.04523 UBA1 ENST00000377269 63.92% 52.79% 11.12% ENST00000490869 22.66% 38.38% 15.72% 0.00778 0.04534 SF3B6 ENST00000478050 19.25% 6.86% 12.39% ENST00000233468 80.75% 93.14% 12.39% 0.007927 0.04606 DTX2 ENST00000467729 24.61% 12.16% 12.45% ENST00000468546 35.34% 47.30% 11.96% 0.007963 0.0462 MICAL3 ENST00000672019 31.03% 19.31% 11.71% ENST00000495076 17.40% 33.29% 15.89% 0.008016 0.04644 CD320 ENST00000598299 15.38% 5.83% 9.54% ENST00000301458 79.78% 94.17% 14.39% 0.008172 0.04727 PKN2 ENST00000370521 67.65% 56.07% 11.58% ENST00000316005 26.43% 38.62% 12.19% 0.008279 0.04782 PKP4 ENST00000389757 19.29% 8.86% 10.43% ENST00000480171 11.46% 23.21% 11.75% 0.008353 0.04811 ATP6V1H ENST00000521335 42.54% 27.27% 15.26% ENST00000521707 29.05% 59.74% 30.69% 0.008459 0.04864 MRPS16 ENST00000471251 13.27% 3.91% 9.36% ENST00000372945 83.87% 94.35% 10.48% 0.008478 0.04868 UBE4B ENST00000253251 12.37% 1.14% 11.22% ENST00000462658 11.17% 30.23% 19.06% 0.008499 0.04872 ATXN2L ENST00000568266 2.02% 0.00% 2.02% ENST00000565845 1.63% 15.00% 13.37% 0.008656 0.04949 SLC25A46 ENST00000355943 38.48% 19.09% 19.38% ENST00000513706 39.82% 58.17% 18.35% 0.008721 0.04956 TFG ENST00000481203 82.38% 72.64% 9.74% ENST00000476228 7.23% 20.24% 13.00% 0.008702 0.04956 VPS39 ENST00000318006 15.90% 6.74% 9.16% ENST00000568029 41.51% 71.15% 29.64% 0.008713 0.04956 ATP13A3 ENST00000645621 22.15% 14.65% 7.50% ENST00000497567 6.03% 16.98% 10.95% 0.008764 0.04973 AL645568.1 ENST00000659863 14.75% 3.57% 11.18% ENST00000661267 23.27% 38.10% 14.82% 0.008824 0.04998

TABLE 7 Endothelial cell specific DCI genes Prevalance Prevalance Prevalance Prevalance of difference of of non-endothelial Prevalance of of Endothelial endothelial endothelial Prevalance cell non-endothelial cell non- cell cell preferred cell preferred difference Non-endothelial preferred cells endothelial preferred isoform in isoform in of endothelial cell isoform preferred cell FDR Gene isoform endothelial non-endothelial cell preferred preferred isoform in isoform preferred adj Name ID cells cells isoform ID endothelial cells in non-endothelial isoform P-values P-values CYTOR ENST00000642451 41.72% 22.95% 18.78% ENST00000414030 7.38% 16.36% 8.98% 6.15E−27 1.89E−23 YWHAH ENST00000397492 95.82% 83.47% 12.35% ENST00000248975 0.20% 12.87% 12.67% 6.48E−12 9.95E−09 NEDD9 ENST00000379433 77.72% 33.45% 44.28% ENST00000504634 0.87% 23.19% 22.320% 1.99E−11 2.03E−08 APOLD1 ENST00000356591 66.07% 9.05% 57.02% ENST00000534843 30.19% 89.16% 58.97% 4.65E−11 3.57E−08 AL008729.1 ENST00000606150 46.67% 9.33% 37.33% ENST00000399446 20.00% 86.67% 66.67% 2.92E−10 1.79E−07 MTREX ENST00000230640 86.33% 44.57% 41.76% ENST00000518955 0.00% 15.31% 15.31% 5.41E−10 2.77E−07 MT2A ENST00000245185 97.46% 79.38% 18.080% ENST00000562017 1.880% 11.05% 9.17% 6.35E−10 2.79E−07 HERPUD1 ENST00000569429 12.70% 1.520% 11.17% ENST00000569569 12.82% 32.11% 19.29% 1.22E−08 4.16E−06 NBEAL1 ENST00000449802 21.52% 11.71% 9.81% ENST00000460355 64.84% 79.96% 15.12% 1.15E−08 4.16E−06 ARHGAP24 ENST00000395183 33.95% 7.01% 26.94% ENST00000509709 2.44% 27.05% 24.62% 4.35E−08 1.34E−05 CD74 ENST00000353334 29.21% 20.99% 8.21% ENST00000523836 12.270% 28.76% 16.49% 7.99E−08 1.89E−05 ETS1 ENST00000527676 47.17% 0.00% 47.17% ENST00000530924 3.77% 29.55% 25.77% 7.81E−08 1.89E−05 IVNS1ABP ENST00000367498 27.30% 3.90% 23.39% ENST00000459929 20.77% 26.80% 6.03% 9.06E−08 1.99E−05 MID1 ENST00000380779 32.98% 0.00% 32.98% ENST00000380780 26.45% 80.90% 54.45% 1.17E−07 2.39E−05 DLD ENST00000417551 62.50% 14.89% 47.61% ENST00000205402 30.56% 76.60% 46.04% 2.45E−07 4.70E−05 DAB2 ENST00000515700 18.66% 1.37% 17.29% ENST00000545653 3.68% 18.00% 14.32% 3.25E−07 5.87E−05 NF1 ENST00000493220 16.85% 10.66% 6.19% ENST00000581113 6.59% 29.95% 23.36% 8.26E−07 0.000133 SENP6 ENST00000447266 53.37% 8.79% 44.57% ENST00000436928 10.42% 30.17% 19.76% 1.37E−06 0.000191 PPDPFL ENST00000303202 14.18% 2.95% 11.24% ENST00000399653 61.40% 77.76% 16.36% 1.53E−06 0.000204 SH3D19 ENST00000604440 17.65% 0.00% 17.65% ENST00000604922 41.18% 100.00% 58.82% 2.83E−06 0.000348 RBP5 ENST00000266560 58.06% 21.48% 36.59% ENST00000542370 12.90% 70.18% 57.27% 3.92E−06 0.000463 CBWD5 ENST00000468198 32.28% 20.13% 12.15% ENST00000382404 5.87% 11.69% 5.81% 4.75E−06 0.000519 FNDC3A ENST00000398316 47.79% 16.82% 30.97% ENST00000378383 19.11% 31.29% 12.18% 4.68E−06 0.000519 RPS2 ENST00000526586 16.22% 1.72% 14.49% ENST00000529806 72.97% 96.55% 23.58% 4.90E−06 0.000519 LIMD1 ENST00000474665 31.580% 1.09% 30.49% ENST00000440097 0.00% 15.22% 15.22% 5.42E−06 0.000555 PLEKHG1 ENST00000644968 33.67% 4.85% 28.81% ENST00000644996 7.50% 48.65% 41.15% 6.32E−06 0.000626 AOPEP ENST00000375315 34.45% 6.90% 27.55% ENST00000473778 5.56% 48.28% 42.720% 7.44E−06 0.000714 COL4A2 ENST00000650225 22.11% 9.23% 12.87% ENST00000400163 5.60% 12.15% 6.55% 9.97E−06 0.0009 EHMT1 ENST00000637318 27.78% 9.42% 18.36% ENST00000495657 28.89% 68.52% 39.63% 9.79E−06 0.0009 ADGRF5 ENST00000283296 61.19% 12.49% 48.70% ENST00000265417 30.75% 80.37% 49.62% 1.15E−05 0.000957 CFLAR ENST00000309955 64.94% 46.09% 18.85% ENST00000490965 8.09% 19.93% 11.84% 1.13E−05 0.000957 SPTBN1 ENST00000602898 26.07% 3.85% 22.22% ENST00000333896 28.22% 56.03% 27.80% 1.37E−05 0.001079 RNF213 ENST00000582970 26.52% 13.23% 13.30% ENST00000319921 3.76% 8.43% 4.68% 1.41E−05 0.001079 LRRFIP1 ENST00000308482 23.67% 14.40% 9.26% ENST00000244815 31.99% 44.85% 12.86% 1.47E−05 0.001098 IFI44L ENST00000370751 68.26% 48.81% 19.45% ENST00000486882 5.08% 23.55% 18.47% 1.64E−05 0.001198 MATR3 ENST00000502394 23.90% 0.91% 22.99% ENST00000505625 57.23% 89.15% 31.920% 1.83E−05 0.001303 MECP2 ENST00000303391 57.50% 21.24% 36.26% ENST00000629277 0.00% 15.93% 15.93% 2.02E−05 0.001408 HPS3 ENST00000296051 87.75% 51.12% 36.64% ENST00000462030 4.51% 42.520% 38.01% 2.32E−05 0.00155 HERC4 ENST00000515753 8.68% 3.49% 5.19% ENST00000492996 15.28% 29.48% 14.20% 2.43E−05 0.001585 H2AFY ENST00000506218 9.59% 0.00% 9.59% ENST00000512507 26.94% 42.67% 15.73% 3.41E−05 0.002182 SELENOS ENST00000526049 67.500% 26.44% 41.06% ENST00000534014 20.45% 39.98% 19.520% 3.48E−05 0.002182 JMJDIC ENST00000399262 34.53% 25.61% 8.91% ENST00000633035 13.61% 29.100% 15.49% 4.70E−05 0.002887 N4BP1 ENST00000569027 29.73% 0.00% 29.73% ENST00000564710 0.00% 17.91% 17.91% 4.95E−05 0.002979 DST ENST00000340834 40.73% 23.410% 17.32% ENST00000487754 10.67% 21.03% 10.36% 5.06E−05 0.00299 COX16 ENST00000555276 67.95% 53.95% 14.00% ENST00000389912 16.67% 35.57% 18.90% 5.19E−05 0.003008 LIMS1 ENST00000544547 26.32% 8.26% 18.06% ENST00000422797 11.59% 24.020% 12.420% 5.37E−05 0.003011 POM121 ENST00000358357 10.370% 2.07% 8.30% ENST00000395270 76.40% 95.92% 19.52% 5.39E−05 0.003011 COL4A1 ENST00000375820 65.28% 47.71% 17.57% ENST00000647632 4.09% 12.27% 8.180% 5.94E−05 0.003236 KLF6 ENST00000542957 25.05% 3.31% 21.740% ENST00000497571 62.24% 92.44% 30.20% 6.01E−05 0.003236 CEMIP2 ENST00000377044 34.950% 7.38% 27.58% ENST00000543165 42.68% 59.24% 16.55% 6.83E−05 0.003614 WIPF1 ENST00000487291 22.220% 11.11% 11.11% ENST00000392547 25.54% 32.06% 6.51% 7.56E−05 0.003937 PNPT1 ENST00000260604 42.57% 2.70% 39.86% ENST00000447944 12.76% 63.28% 50.52% 8.83E−05 0.004465 USP15 ENST00000280377 19.74% 5.08% 14.66% ENST00000537297 12.85% 33.20% 20.35% 9.72E−05 0.004815 MIR29B2CHG ENST00000657366 14.71% 0.00% 14.71% ENST00000608023 85.29% 98.20% 12.91% 0.0001 0.004907 PTPRE ENST00000455661 31.85% 21.76% 10.09% ENST00000463727 16.91% 47.84% 30.93% 0.0001 0.004907 S100A4 ENST00000354332 12.50% 0.61% 11.89% ENST00000368716 81.25% 96.95% 15.70% 0.00011 0.004945 PHACTR1 ENST00000482982 32.89% 6.73% 26.16% ENST00000332995 22.95% 46.21% 23.26% 0.00011 0.00495 UPF3A ENST00000475218 16.64% 5.96% 10.68% ENST00000492270 3.01% 25.13% 22.12% 0.00012 0.005593 NPIPA1 ENST00000472413 63.16% 22.90% 40.26% ENST00000328085 11.84% 44.10% 32.26% 0.00013 0.005621 VAPA ENST00000577901 36.43% 22.73% 13.70% ENST00000400000 31.43% 62.30% 30.87% 0.00015 0.00637 DNAJB4 ENST00000370763 86.640% 25.00% 61.64% ENST00000484662 6.900% 60.00% 53.10% 0.00015 0.006589 TBCA ENST00000651106 10.53% 0.90% 9.62% ENST00000380377 80.70% 92.63% 11.92% 0.00015 0.006589 ITGA1 ENST00000650673 43.530% 32.31% 11.22% ENST00000504669 20.24% 26.93% 6.68% 0.00018 0.007552 PRKCE ENST00000480633 41.580% 1.49% 40.09% ENST00000497602 0.00% 16.42% 16.42% 0.00018 0.007552 CNP ENST00000592861 32.86% 6.45% 26.41% ENST00000393892 65.20% 93.55% 28.35% 0.00019 0.007683 DDB2 ENST00000612309 8.580% 0.00% 8.58% ENST00000617847 8.54% 21.47% 12.93% 0.00023 0.009218 CD163L1 ENST00000545597 22.78% 5.63% 17.15% ENST00000543276 19.230% 57.14% 37.91% 0.00025 0.009905 DLC1 ENST00000358919 14.74% 3.37% 11.37% ENST00000511869 0.00% 32.45% 32.45% 0.00028 0.010624 LINC-PINT ENST00000435523 16.77% 6.25% 10.53% ENST00000647388 3.23% 23.49% 20.27% 0.00028 0.010624 MRPS21 ENST00000581066 68.36% 42.31% 26.05% ENST00000614145 31.64% 57.69% 26.05% 0.00028 0.010624 PIGX ENST00000392391 57.14% 18.60% 38.54% ENST00000426755 42.86% 74.42% 31.56% 0.00029 0.010632 RGPD6 ENST00000329516 56.240% 35.51% 20.73% ENST00000463822 16.13% 28.17% 12.03% 0.00032 0.011495 SLFN12 ENST00000452764 94.74% 29.03% 65.70% ENST00000304905 0.00% 45.16% 45.16% 0.00033 0.011727 DARS ENST00000478212 18.52% 2.06% 16.46% ENST00000264161 45.70% 66.11% 20.41% 0.00037 0.012595 POMP ENST00000460403 13.75% 2.53% 11.22% ENST00000380842 86.25% 97.47% 11.22% 0.00036 0.012595 USP48 ENST00000471752 22.50% 2.82% 19.68% ENST00000374732 13.14% 28.40% 15.26% 0.00038 0.013083 ST6GAL1 ENST00000485105 13.22% 8.27% 4.94% ENST00000448408 67.02% 82.50% 15.48% 0.00039 0.013269 GOLGA8B ENST00000569100 27.57% 4.21% 23.36% ENST00000567956 9.92% 18.94% 9.02% 0.0004 0.013503 NDUFAF6 ENST00000396111 12.20% 0.96% 11.23% ENST00000521840 64.63% 70.13% 5.50% 0.00041 0.013509 H3F3B ENST00000591893 34.51% 18.33% 16.17% ENST00000254810 58.95% 81.67% 22.72% 0.00045 0.01428 KTN1 ENST00000556631 13.62% 3.92% 9.70% ENST00000554831 25.48% 41.34% 15.86% 0.00044 0.01428 OSMR ENST00000502536 20.43% 2.63% 17.800% ENST00000274276 69.99% 90.35% 20.35% 0.00045 0.01428 CBWD6 ENST00000457288 17.20% 4.56% 12.64% ENST00000617722 16.82% 24.71% 7.89% 0.00046 0.014586 PCGF3 ENST00000430644 37.04% 8.86% 28.18% ENST00000362003 53.55% 77.68% 24.12% 0.00048 0.015194 PELI1 ENST00000358912 79.63% 50.71% 28.92% ENST00000468869 1.89% 28.24% 26.35% 0.00054 0.016651 KPNA5 ENST00000368564 78.98% 55.73% 23.240% ENST00000413340 11.11% 43.21% 32.10% 0.00055 0.01688 SNHG7 ENST00000414282 24.240% 1.45% 22.79% ENST00000416970 0.00% 17.39% 17.39% 0.00057 0.017447 TFRC ENST00000420415 19.07% 10.51% 8.56% ENST00000392396 16.77% 31.22% 14.45% 0.00058 0.017582 TUBB6 ENST00000587204 31.77% 13.16% 18.61% ENST00000591909 30.70% 64.91% 34.21% 0.00063 0.018518 CHCHD1 ENST00000372837 40.91% 10.96% 29.95% ENST00000372833 59.09% 89.04% 29.95% 0.00078 0.022789 TNFRSF14 ENST00000482602 20.83% 1.49% 19.34% ENST00000463471 32.68% 64.18% 31.50% 0.00079 0.022789 NRP1 ENST00000374867 10.630% 3.41% 7.23% ENST00000432372 19.14% 35.13% 15.99% 0.0008 0.023049 TCERG1 ENST00000509787 14.81% 0.80% 14.01% ENST00000509810 5.93% 15.28% 9.36% 0.00088 0.024878 CA2 ENST00000520996 17.56% 3.74% 13.82% ENST00000285379 78.85% 96.26% 17.41% 0.00093 0.025763 PYGO2 ENST00000483463 84.00% 51.85% 32.15% ENST00000368457 14.52% 48.15% 33.63% 0.00093 0.025763 BMPR2 ENST00000374580 75.820% 18.5% 57.31% ENST00000479069 20.00% 79.16% 59.16% 0.00104 0.028145 MPC2 ENST00000271373 15.00% 0.00% 15.00% ENST00000367846 85.00% 100.00% 15.00% 0.00103 0.028145 NUTM2A-AS1 ENST00000668225 7.14% 0.93% 6.22% ENST00000654503 19.93% 36.91% 16.98% 0.00106 0.028584 CDR2 ENST00000564542 30.00% 10.23% 19.77% ENST00000569045 54.00% 77.92% 23.92% 0.00114 0.030333 FGD4 ENST00000473513 9.93% 2.59% 7.34% ENST00000525053 3.11% 26.15% 23.04% 0.00115 0.03044 RECQL ENST00000539672 27.50% 0.00% 27.50% ENST00000444129 72.50% 100.00% 27.50% 0.00116 0.03044 MICOS10 ENST00000498067 16.09% 4.38% 11.70% ENST00000322753 76.95% 82.60% 5.650% 0.00117 0.030466 SF3B6 ENST00000478050 22.22% 1.33% 20.89% ENST00000233468 77.78% 98.67% 20.89% 0.00121 0.031114 ZNF518A ENST00000484770 71.09% 59.57% 11.52% ENST00000563195 1.23% 14.81% 13.58% 0.00131 0.033582 AC027097.2 ENST00000588925 40.00% 11.71% 28.29% ENST00000660188 0.00% 54.35% 54.35% 0.00137 0.034241 COBLL1 ENST00000439313 42.11% 5.45% 36.65% ENST00000480873 5.57% 16.42% 10.84% 0.00136 0.034241 NDUFAB1 ENST00000570319 5.77% 0.00% 5.77% ENST00000007516 86.54% 97.71% 11.18% 0.0014 0.034445 PDE7B ENST00000615259 57.10% 29.82% 27.28% ENST00000308191 36.08% 68.42% 32.34% 0.0014 0.034445 DSTN ENST00000449141 31.71% 9.59% 22.12% ENST00000246069 46.34% 78.08% 31.74% 0.00164 0.039304 MAP3K8 ENST00000375321 14.99% 2.20% 12.790% ENST00000413724 26.09% 58.59% 32.51% 0.00166 0.039403 FAM13B ENST00000513640 26.32% 4.49% 21.82% ENST00000033079 36.84% 55.06% 18.21% 0.00171 0.040306 CAVI ENST00000341049 72.72% 23.40% 49.32% ENST00000393467 18.18% 61.70% 43.52% 0.00176 0.040881 DYRK2 ENST00000542503 31.58% 3.33% 28.25% ENST00000344096 68.42% 96.67% 28.25% 0.00181 0.040881 MCL1 ENST00000620947 13.24% 0.01% 13.22% ENST00000369026 76.23% 94.73% 18.50% 0.00181 0.040881 NBPF10 ENST00000583866 57.50% 30.42% 27.07% ENST00000612520 37.50% 66.05% 28.55% 0.00181 0.040881 SRGAP2 ENST00000604423 22.64% 11.28% 11.35% ENST00000624686 44.86% 64.59% 19.73% 0.00179 0.040881 TRAPPC12 ENST00000452495 25.00% 4.00% 21.00% ENST00000497597 40.00% 66.67% 26.67% 0.00194 0.043458 CCDC47 ENST00000582331 29.41% 1.82% 27.59% ENST00000584112 5.88% 21.82% 15.94% 0.00199 0.044039 ANAPC5 ENST00000535463 8.57% 0.00% 8.57% ENST00000538334 24.71% 45.74% 21.03% 0.00209 0.045864 WDR59 ENST00000563111 17.97% 3.76% 14.21% ENST00000569421 9.38% 26.40% 17.03% 0.00217 0.047292 PGM2L1 ENST00000622957 57.11% 32.14% 24.97% ENST00000298198 42.89% 67.86% 24.97% 0.00227 0.048659 SWAP70 ENST00000534662 20.53% 3.64% 16.89% ENST00000531814 2.81% 17.73% 14.92% 0.00225 0.048659

TABLE 8 Myeloid cell specific DCI genes Prevalance of Prevalance Prevalance Prevalance non-myeloid difference of of cell Prevalance of Myeloid myeloid myeloid Prevalance preferred of non- cell cell preferred cell preferred difference Non-myeloid isoform non-myeloid cell myeloid preferred isoform in isoform in of cell in preferred isoform cell FDR Gene isoform myeloid non-myeloid myeloid cell preferred isoform myeloid in non-myeloid preferred adj Name ID cells cells preferred isoform ID cells cells isoform P-values P-values CD74 ENST00000523836 33.75% 18.45% 15.30% ENST00000353334 14.60% 30.19% 15.59% 7.59E−26 1.93E−22 CSTB ENST00000640406 16.40% 2.49% 13.91% ENST00000291568 77.54% 97.08% 19.54% 6.72E−23 8.55E−20 CARMIL1 ENST00000461945 97.53% 18.67% 78.86% ENST00000329474 2.47% 77.81% 75.34% 7.34E−21 6.22E−18 YWHAH ENST00000248975 21.04% 0.37% 20.66% ENST00000397492 72.16% 95.87% 23.71% 5.29E−19 3.36E−16 MT2A ENST00000561491 12.93% 4.05% 8.88% ENST00000245185 70.63% 86.86% 16.22% 9.13E−16 4.64E−13 FYB1 ENST00000512982 13.27% 5.48% 7.78% ENST00000646444 10.40% 28.81% 18.40% 5.20E−13 2.21E−10 HLA-DPA1 ENST00000417724 33.67% 18.08% 15.59% ENST00000419277 52.31% 72.29% 19.98% 9.79E−09 3.56E−06 AL3547401 ENST00000429998 62.64% 26.16% 36.47% ENST00000593917 32.97% 70.77% 37.80% 2.52E−08 8.02E−06 MTREX ENST00000518955 23.81% 4.63% 19.18% ENST00000230640 22.42% 73.60% 51.17% 3.58E−08 1.01E−05 SMCHD1 ENST00000577300 18.94% 10.48% 8.46% ENST00000320876 22.52% 40.55% 18.03% 4.00E−08 1.02E−05 CCL3 ENST00000613922 84.19% 44.83% 39.35% ENST00000614051 15.12% 54.25% 39.12% 4.84E−08 1.03E−05 PRDM1 ENST00000424894 9.08% 0.17% 8.92% ENST00000481163 9.46% 24.83% 15.37% 9.11E−08 1.78E−05 NAMPT ENST00000486949 23.80% 11.86% 11.93% ENST00000222553 26.21% 45.25% 19.04% 3.71E−07 6.74E−05 PTPRE ENST00000463727 57.49% 19.79% 37.70% ENST00000455661 5.07% 35.88% 30.81% 4.88E−07 7.76E−05 ZFYVE16 ENST00000512558 25.40% 13.45% 11.94% ENST00000505560 14.30% 41.22% 26.93% 4.78E−07 7.76E−05 GLS ENST00000320717 54.23% 33.47% 20.77% ENST00000338435 22.83% 43.73% 20.90% 2.68E−06 0.00038 CBWD5 ENST00000382404 17.82% 7.85% 9.97% ENST00000468198 7.47% 28.12% 20.65% 3.04E−06 0.00041 RASGRP3 ENST00000484909 38.89% 19.83% 19.06% ENST00000468856 0.00% 11.58% 11.58% 3.34E−06 0.00041 TMEM176B ENST00000492607 36.76% 9.89% 26.87% ENST00000326442 36.77% 74.78% 38.01% 3.39E−06 0.00041 LIMS1 ENST00000393310 34.70% 8.64% 26.06% ENST00000544547 6.83% 19.77% 12.94% 3.78E−06 0.00044 GNPAT ENST00000436239 19.23% 0.00% 19.23% ENST00000366647 62.66% 86.50% 23.84% 4.18E−06 0.00046 ATP5PD ENST00000580649 14.91% 2.47% 12.44% ENST00000301587 85.09% 97.28% 12.19% 5.06E−06 0.00054 DAB2 ENST00000545653 19.12% 3.70% 15.42% ENST00000515700 1.47% 15.43% 13.96% 6.17E−06 0.00063 ERN1 ENST00000584041 13.33% 2.55% 10.78% ENST00000606895 22.22% 39.21% 16.99% 8.22E−06 0.0008 IFI30 ENST00000600463 29.97% 11.73% 18.24% ENST00000407280 70.03% 88.27% 18.24% 8.82E−06 0.00083 CCNH ENST00000508855 15.48% 3.40% 12.08% ENST00000607486 5.62% 24.00% 18.38% 1.04E−05 0.00091 TCF25 ENST00000563636 11.23% 1.83% 9.40% ENST00000564652 23.78% 35.64% 11.86% 1.02E−05 0.00091 SMAP2 ENST00000435168 44.44% 3.92% 40.52% ENST00000372718 13.78% 68.63% 54.850% 1.19E−05 0.00101 NAIP ENST00000503719 10.98% 1.52% 9.46% ENST00000508794 16.80% 30.40% 13.60% 1.38E−05 0.0011 DDX5 ENST00000581230 91.87% 80.29% 11.57% ENST00000225792 3.16% 17.10% 13.94% 1.43E−05 0.0011 TNFAIP8 ENST00000504771 20.68% 7.21% 13.47% ENST00000513374 41.71% 75.87% 34.16% 2.05105 0.00153 CYTOR ENST00000413202 22.36% 7.89% 14.47% ENST00000642451 16.25% 31.75% 15.50% 3.21E−05 0.00233 PDE4DIP ENST00000616206 23.73% 5.00% 18.73% ENST00000529945 19.37% 38.43% 19.06% 3.33E−05 0.00234 VPS13C ENST00000249837 33.62% 16.54% 17.08% ENST00000559119 17.85% 42.28% 24.43% 3.41E−05 0.00234 DSE ENST00000606265 15.24% 2.17% 13.06% ENST00000647046 6.05% 36.96% 30.91% 3.72E−05 0.00249 SND1 ENST00000492840 28.37% 1.42% 26.95% ENST00000489417 0.00% 12.72% 12.72% 5.04E−05 0.00329 MIB1 ENST00000577749 46.67% 8.33% 38.33% ENST00000261537 46.67% 88.09% 41.43% 6.43E−05 0.00409 PPDPFL ENST00000399653 88.21% 69.95% 18.26% ENST00000517663 5.46% 24.32% 18.86% 7.34E−05 0.00455 NRP1 ENST00000432372 43.27% 19.05% 24.22% ENST00000374816 7.94% 17.38% 9.44% 7.87E−05 0.00477 NBPF10 ENST00000612520 71.29% 52.38% 18.91% ENST00000583866 23.94% 45.28% 21.34% 8.51E−05 0.00503 TAOK3 ENST00000542902 21.43% 1.61% 19.82% ENST00000537952 2.38% 24.56% 22.180% 9.17E−05 0.0053 UGT1A1 ENST00000360418 30.96% 12.41% 18.54% ENST00000305208 69.040% 87.59% 18.54% 9.46E−05 0.00535 ITGA4 ENST00000397033 53.74% 40.82% 12.93% ENST00000476089 8.28% 20.19% 11.910% 0.0001 0.00555 PPA1 ENST00000610026 62.96% 30.00% 32.96% ENST00000373232 20.080% 65.000% 44.920% 0.00011 0.00594 CKLF ENST00000526149 26.31% 4.05% 22.26% ENST00000264001 7.90% 25.590% 17.70% 0.00013 0.00617 OSTC ENST00000505745 30.23% 5.44% 24.79% ENST00000361564 69.77% 86.62% 16.85% 0.00012 0.00617 SLC38A2 ENST00000551405 10.53% 1.11% 9.42% ENST00000256689 78.82% 90.96% 12.14% 0.00013 0.00617 SRP19 ENST00000282999 35.71% 5.16% 30.56% ENST00000503445 0.00% 14.29% 14.29% 0.00014 0.0065 PNKP ENST00000629179 39.13% 3.95% 35.18% ENST00000626274 30.43% 67.85% 37.42% 0.00015 0.00719 HA VCR2 ENST00000522902 51.52% 25.93% 25.60% ENST00000524219 6.11% 20.37% 14.26% 0.00016 0.00734 PIGX ENST00000426755 88.64% 52.86% 35.78% ENST00000392391 6.82% 41.43% 34.61% 0.00023 0.01028 AC243829.4 ENST00000616926 98.44% 72.79% 25.64% ENST00000610845 0.00% 20.87% 20.87% 0.00023 0.0103 HIF 1A ENST00000337138 45.42% 27.01% 18.41% ENST00000547430 13.10% 30.56% 17.46% 0.00027 0.0117 SNX29 ENST00000564111 25.81% 7.87% 17.94% ENST00000564791 18.29% 31.98% 13.69% 0.00029 0.01246 TFEC ENST00000265440 27.08% 2.29% 24.79% ENST00000484212 34.950% 57.10% 22.15% 0.00029 0.01246 RABGAP1 ENST00000456584 35.48% 7.18% 28.30% ENST00000373647 41.94% 61.42% 19.48% 0.00036 0.01503 AL0087291 ENST00000399446 84.21% 47.92% 36.29% ENST00000606150 10.53% 31.25% 20.72% 0.00038 0.01541 PGF ENST00000555253 54.41% 24.76% 29.66% ENST00000238607 32.89% 50.97% 18.07% 0.00041 0.01644 GTF2I ENST00000491325 16.85% 4.58% 12.27% ENST00000614048 8.99% 18.07% 9.08% 0.00044 0.01733 MOBIA ENST00000463975 36.42% 10.69% 25.73% ENST00000396049 58.02% 68.70% 10.68% 0.00048 0.01829 SAMHD1 ENST00000645444 43.19% 10.17% 33.02% ENST00000643161 0.00% 15.25% 15.25% 0.00049 0.01829 SYNGR2 ENST00000592456 45.83% 2.44% 43.39% ENST00000225777 33.33% 78.05% 44.72% 0.00048 0.01829 DDX60L ENST00000504793 11.01% 0.00% 11.01% ENST00000513103 0.00% 29.46% 29.46% 0.00051 0.01873 UBAC2 ENST00000460562 24.44% 3.26% 21.18% ENST00000376440 4.44% 22.83% 18.38% 0.00058 0.02105 SRGAP2 ENST00000624686 67.89% 49.23% 18.66% ENST00000604423 9.28% 20.20% 10.92% 0.00066 0.02352 UGT1A4 ENST00000450233 22.78% 8.18% 14.60% ENST00000373409 77.22% 91.82% 14.60% 0.00069 0.0244 RHOBTB3 ENST00000504949 11.66% 3.11% 8.56% ENST00000379982 65.14% 82.35% 17.21% 0.00074 0.02564 UGT1A6 ENST00000406651 20.33% 7.54% 12.78% ENST00000305139 74.18% 90.09% 15.91% 0.00075 0.02564 IFIT3 ENST00000371818 63.63% 26.52% 37.11% ENST00000371811 36.37% 73.48% 37.11% 0.0008 0.02709 OXSR1 ENST00000483695 22.22% 2.50% 19.72% ENST00000311806 55.56% 77.50% 21.94% 0.00081 0.02709 KLF6 ENST00000497571 99.97% 70.23% 29.73% ENST00000542957 0.03% 18.30% 18.27% 0.00085 0.02823 HOOK2 ENST00000593143 94.61% 83.72% 10.89% ENST00000589134 1.16% 10.63% 9.47% 0.00088 0.02871 DST ENST00000370765 19.100% 10.01% 9.09% ENST00000340834 12.82% 38.47% 25.65% 0.00094 0.02921 SMIM4 ENST00000476842 53.13% 14.58% 38.540% ENST00000477703 15.63% 50.000% 34.38% 0.00091 0.02921 STAT1 ENST00000392323 27.71% 12.250% 15.46% ENST00000415035 23.14% 33.30% 10.17% 0.00094 0.02921 IVNS1ABP ENST00000475046 10.20% 4.10% 6.10% ENST00000367498 3.67% 25.91% 22.24% 0.00098 0.03003 CSNK2A1 ENST00000608066 20.59% 0.83% 19.75% ENST00000645334 0.00% 18.80% 18.80% 0.00101 0.03056 CLK3 ENST00000564353 47.06% 17.24% 29.82% ENST00000563418 29.41% 77.59% 48.17% 0.00109 0.03235 ATG7 ENST00000470474 11.83% 1.27% 10.56% ENST00000354449 28.05% 60.000% 31.95% 0.00126 0.03614 RBBP4 ENST00000465780 18.18% 6.42% 11.76% ENST00000373493 61.36% 78.61% 17.25% 0.00125 0.03614 HSPD1 ENST00000491249 25.85% 4.30% 21.55% ENST00000426480 5.02% 15.62% 10.60% 0.00134 0.03756 GOLGA8R ENST00000624918 81.48% 39.02% 42.46% ENST00000327271 18.52% 60.98% 42.46% 0.00137 0.03794 ATP5MC1 ENST00000393366 44.02% 33.15% 10.86% ENST00000355938 45.30% 58.84% 13.54% 0.00144 0.03909 UGT1A10 ENST00000373445 24.41% 11.93% 12.48% ENST00000344644 75.59% 88.07% 12.48% 0.00149 0.03992 RGPD6 ENST00000437167 18.05% 4.85% 13.20% ENST00000329516 17.21% 48.91% 31.70% 0.00153 0.04051 SF3A3 ENST00000461869 17.65% 0.00% 17.65% ENST00000373019 58.82% 90.54% 31.72% 0.00154 0.04051 UBR1 ENST00000546274 13.04% 2.61% 10.43% ENST00000290650 56.52% 74.78% 18.26% 0.00186 0.04738 SOX6 ENST00000530378 16.31% 1.47% 14.84% ENST00000533870 0.00% 23.53% 23.53% 0.0019 0.04779 SAFB2 ENST00000590000 20.00% 10.98% 9.02% ENST00000592599 0.00% 18.85% 18.85% 0.00198 0.04936 CBLB ENST00000476370 43.44% 31.13% 12.31% ENST00000646499 24.22% 31.35% 7.13% 0.00203 0.0496

TABLE 9 Follicular B cell specific DCI genes Prevalance of Prevalance Prevalance Prevalance non-follicular difference of of cell Prevalance of Follicular follicular follicular Prevalance preferred of non- cell cell preferred cell preferred difference Non-follicular isoform non-follicular cell follicular preferred isoform in isoform in of cell in preferred isoform cell FDR Gene isoform follicular non-follicular follicular cell preferred isoform follicular in non-follicular preferred adj Name ID cells cells preferred isoform ID cells cells isoform P-values P-values IGHG1 ENST00000390548 26.06% 1.13% 24.93% ENST00000390549 72.20% 98.780% 26.58% 6.53E−138 8.00E−135 IGHG3 ENST00000641136 27.67% 1.14% 26.53% ENST00000390551 72.33% 98.86% 26.53% 1.85E−113 7.56E−111 IGHG2 ENST00000641095 30.44% 1.44% 29.00% ENST00000390545 69.56% 98.56% 29.00% 1.66E−108 5.08E−106 CD74 ENST00000523836 36.65% 24.36% 12.29% ENST00000009530 26.51% 40.52% 14.01% 6.01E−19 1.47E−16 NOTCH2 ENST00000650638 56.76% 4.59% 52.17% ENST00000256646 21.62% 38.99% 17.37% 5.23E−15 1.07E−12 ARHGAP24 ENST00000509709 38.09% 8.45% 29.63% ENST00000264343 18.47% 39.64% 21.17% 9.81E−11 1.50E−08 IGHA2 ENST00000497872 46.67% 1.72% 44.94% ENST00000390539 53.33% 98.28% 44.94% 2.98E−09 4.05E−07 ACTR2 ENST00000471552 36.36% 4.55% 31.82% ENST00000260641 18.18% 79.36% 61.18% 5.39E−09 6.60E−07 BCAS4 ENST00000371608 25.45% 7.44% 18.01% ENST00000463943 69.09% 92.56% 23.47% 2.16E−07 2.41E−05 GOLGA8A ENST00000569781 20.51% 3.56% 16.96% ENST00000473125 64.35% 94.05% 29.70% 4.02E−07 4.10E−05 MLLT6 ENST00000620609 25.00% 2.08% 22.92% ENST00000621332 43.75% 78.24% 34.49% 7.14E−07 6.72E−05 MT2A ENST00000245185 100.00% 80.58% 19.42% ENST00000562017 0.00% 10.49% 10.49% 3.38E−06 0.0003 MICOS10 ENST00000498642 15.09% 2.11% 12.99% ENST00000322753 67.92% 82.90% 14.98% 3.96E−05 0.00303 PAWR ENST00000547016 25.00% 0.00% 25.00% ENST00000551712 0.00% 19.44% 19.44% 0.00014 0.00871 RGPD6 ENST00000463822 43.81% 19.25% 24.56% ENST00000329516 21.59% 48.43% 26.84% 0.00014 0.00871 TMEM131 ENST00000485245 26.25% 12.96% 13.29% ENST00000186436 56.25% 79.990% 23.74% 0.00013 0.00871 INPP5D ENST00000359570 19.99% 6.34% 13.65% ENST00000496402 1.79% 20.62% 18.84% 0.00017 0.00945 PIK3AP1 ENST00000468783 30.01% 2.04% 27.97% ENST00000339364 20.07% 57.820% 37.75% 0.00017 0.00945 RGPD5 ENST00000477523 35.56% 12.530% 23.02% ENST00000016946 18.48% 30.86% 12.38% 0.00021 0.01099 LINC-PINT ENST00000647388 27.91% 16.82% 11.09% ENST00000451786 2.40% 14.70% 12.30% 0.00024 0.01219 KMT2A ENST00000648029 11.11% 0.00% 11.11% ENST00000534358 30.55% 38.61% 8.05% 0.00029 0.01346 SRSF6 ENST00000662078 10.53% 0.00% 10.53% ENST00000244020 89.47% 98.63% 9.16% 0.00028 0.01346 SP110 ENST00000463022 16.77% 0.00% 16.77% ENST00000489597 0.00% 8.42% 8.42% 0.0004 0.01737 COX16 ENST00000557612 25.00% 2.94% 22.06% ENST00000555276 50.00% 59.35% 9.35% 0.00047 0.01964 PUM3 ENST00000382032 7.69% 0.00% 7.69% ENST00000469168 0.00% 13.07% 13.07% 0.00048 0.01964 FAM214A ENST00000561490 22.22% 1.58% 20.64% ENST00000562351 29.63% 46.51% 16.88% 0.00051 0.0202 ARL17B ENST00000570618 12.11% 0.76% 11.36% ENST00000622877 2.37% 8.41% 6.05% 0.00055 0.02088 PLEKHG1 ENST00000644996 59.09% 12.67% 46.42% ENST00000358517 15.33% 38.09% 22.77% 0.00063 0.02318 DANCR ENST00000653147 14.29% 0.54% 13.74% ENST00000411630 85.71% 95.65% 9.93% 0.00071 0.02541 GTF2H2 ENST00000518898 30.43% 4.45% 25.98% ENST00000274400 13.04% 39.25% 26.20% 0.0011 0.03539 TFEC ENST00000484212 57.89% 37.71% 20.18% ENST00000265440 1.22% 24.08% 22.86% 0.00109 0.03539 CD69 ENST00000543147 43.96% 21.07% 22.89% ENST00000228434 35.15% 56.99% 21.84% 0.00124 0.039 LTB ENST00000446745 30.56% 20.59% 9.97% ENST00000429299 55.56% 77.96% 22.41% 0.00138 0.04165 SRSF4 ENST00000605204 14.70% 3.31% 11.39% ENST00000373795 9.33% 21.09% 11.76% 0.0014 0.04165 CHD9 ENST00000564582 24.24% 3.33% 20.91% ENST00000565442 26.93% 40.32% 13.39% 0.00145 0.04218 FUT8 ENST00000556518 22.06% 3.54% 18.52% ENST00000553924 7.14% 21.83% 14.69% 0.00156 0.0444 1-Mar ENST00000511245 11.51% 0.47% 11.04% ENST00000274056 2.87% 11.83% 8.96% 0.00174 0.04837 CBWD6 ENST00000486387 22.29% 12.38% 9.92% ENST00000611553 1.72% 14.58% 12.85% 0.00182 0.04945

TABLE 10 T&NK cell specific DCI genes Prevalance Prevalance Prevalance of of of Prevalance Prevalance T&NK T&NK non-T&NK of difference cell cell cell non-T&NK of T&NK preferred preferred Prevalance preferred cell non- cell isoform isoform difference Non-T&NK isoform preferred T&NK preferred in in of cell in isoform cell FDR Gene isoform T&NK non-T&NK T&NK cell preferred isoform T&NK in non-T&NK preferred adj Name ID cells cells preferred isoform ID cells cells isoform P-values P-values CD74 ENST00000353334 41.93% 19.81% 22.12% ENST00000523836 7.80% 28.53% 20.73% 1.52E−18 1.66E−15 MT2A ENST00000245185 98.83% 77.95% 20.88% ENST00000562017 0.39% 11.97% 11.58% 4.13E−18 2.26E−15 CYTOR ENST00000331944 22.57% 7.87% 14.70% ENST00000642451 13.49% 33.43% 19.94% 8.72E−16 3.19E−13 PRDM1 ENST00000481163 53.49% 16.32% 37.17% ENST00000369096 25.32% 62.87% 37.55% 1.67E−14 4.58E−12 AKAP13 ENST00000559820 19.67% 4.37% 15.30% ENST00000394518 1.47% 10.22% 8.75% 9.65E−11 2.11E−08 FYB1 ENST00000515010 22.940% 7.05% 15.90% ENST00000512982 6.10% 11.54% 5.44% 1.27E−08 1.99E−06 HLA-DPA1 ENST00000419277 86.40% 58.01% 28.39% ENST00000417724 9.54% 28.89% 19.35% 1.91E−08 2.61E−06 CYB5A ENST00000340533 98.48% 87.66% 10.82% ENST00000583418 0.24% 9.36% 9.11% 2.40E−08 2.92E−06 HMGN3 ENST00000275036 35.08% 19.52% 15.55% ENST00000620514 46.61% 69.43% 22.820% 2.69108 2.95E−06 AC005670.3 ENST00000663210 13.510% 3.48% 10.03% ENST00000658121 43.240% 76.57% 33.32% 5.85E−07 5.83E−05 A2M ENST00000472360 18.890% 6.80% 12.09% ENST00000318602 60.32% 75.140% 14.82% 1.06E−06 8.91E−05 VPS13C ENST00000559119 56.85% 29.49% 27.36% ENST00000249837 4.980% 25.74% 20.76% 1.69E−06 0.000133 ITGAE ENST00000263087 77.70% 46.03% 31.66% ENST00000570360 0.00% 15.87% 15.87% 2.11E−06 0.000154 CCL3 ENST00000614051 55.27% 22.98% 32.29% ENST00000613922 43.59% 76.49% 32.90% 5.20E−06 0.000356 PDS5A ENST00000503867 18.75% 0.53% 18.22% ENST00000303538 50.00% 59.94% 9.94% 8.86E−06 0.000571 ITGA4 ENST00000476089 24.09% 10.65% 13.44% ENST00000397033 31.89% 53.71% 21.83% 1.36E−05 0.00083 NBEAL1 ENST00000460355 94.15% 71.98% 22.17% ENST00000449802 4.190% 16.43% 12.25% 1.86E−05 0.001073 BHLHE40-AS1 ENST00000663474 7.58% 0.75% 6.82% ENST00000668962 84.850% 98.50% 13.65% 2.61E−05 0.00143 ATM ENST00000530958 43.10% 28.08% 15.03% ENST00000531525 8.62% 15.030% 6.40% 3.04E−05 0.001588 SEC61B ENST00000223641 100.00% 80.02% 19.980% ENST00000481573 0.00% 19.43% 19.43% 4.54E−05 0.002261 AC243829.4 ENST00000610845 22.46% 3.45% 19.01% ENST00000616926 69.91% 95.40% 25.49% 9.99E−05 0.004209 HA VCR2 ENST00000522593 33.83% 10.52% 23.310% ENST00000522902 25.37% 45.640% 20.26% 0.000112 0.004394 ASXL2 ENST00000673455 52.94% 13.33% 39.61% ENST00000435504 31.80% 65.00% 33.19% 0.000163 0.006162 IFITM2 ENST00000399815 90.30% 80.18% 10.12% ENST00000602569 3.53% 7.35% 3.82% 0.000246 0.008411 HMGB1 ENST00000341423 94.23% 71.92% 22.31% ENST00000399489 0.00% 13.74% 13.74% 0.000289 0.009604 MZB1 ENST00000302125 95.00% 53.26% 41.74% ENST00000503120 2.50% 41.71% 39.21% 0.000447 0.014012 MED13 ENST00000580896 9.43% 0.55% 8.88% ENST00000397786 83.02% 95.31% 12.29% 0.000495 0.014479 DOCK10 ENST00000472652 18.60% 2.09% 16.51% ENST00000644695 7.01% 14.58% 7.57% 0.000871 0.022236 ANKRD36 ENST00000421946 56.37% 39.41% 16.97% ENST00000652721 13.01% 25.20% 12.19% 0.001221 0.029081 RBM5 ENST00000494360 17.50% 3.81% 13.69% ENST00000464087 27.50% 37.73% 10.23% 0.001315 0.030676 Clorf122 ENST00000373042 46.87% 23.11% 23.77% ENST00000373043 25.00% 60.23% 35.23% 0.001486 0.033931 IFI30 ENST00000407280 100.00% 73.15% 26.85% ENST00000600463 0.00% 26.85% 26.85% 0.001524 0.034087 CD69 ENST00000228434 60.52% 43.49% 17.02% ENST00000543147 18.15% 34.11% 15.96% 0.001566 0.034335 FARP1 ENST00000319562 70.59% 17.54% 53.05% ENST00000596580 20.59% 50.35% 29.76% 0.002 0.041451 CASP4 ENST00000534356 25.81% 2.33% 23.48% ENST00000533730 0.00% 5.33% 5.33% 0.002151 0.043657 HMGN4 ENST00000477243 11.76% 0.00% 11.76% ENST00000377575 88.24% 100.00% 11.76% 0.002278 0.045349 AGO3 ENST00000324350 22.92% 13.04% 9.87% ENST00000373191 48.07% 70.42% 22.35% 0.002317 0.045349 SP100 ENST00000494901 21.21% 8.41% 12.80% ENST00000409897 5.68% 11.56% 5.88% 0.002633 0.049763

TABLE 11 Plasma B cell specific DCI genes Prevalance of Prevalance Prevalance Prevalance non-plasma difference of of cell Prevalance of Plasma plasma plasma Prevalance preferred of non- cell cell preferred cell preferred difference Non-plasma isoform non-plasma cell plasma preferred isoform in isoform in of cell in preferred isoform cell FDR Gene isoform plasma non-plasma plasma cell preferred isoform plasma in non-plasma preferred adj Name ID cells cells preferred isoform ID cells cells isoform P-values P-values CFLAR ENST00000490965 39.08% 11.58% 27.50% ENST00000309955 24.85% 57.48% 32.63% 2.86E−19 1.22E−16 CCDC144A ENST00000340621 33.58% 2.62% 30.95% ENST00000399273 41.40% 79.12% 37.72% 4.55E−14 9.72E−12 CYTOR ENST00000646865 18.40% 2.93% 15.47% ENST00000642451 7.08% 32.16% 25.08% 2.18E−13 4.00E−11 HMGB1 ENST00000399489 29.30% 6.18% 23.13% ENST00000341423 54.51% 82.12% 27.61% 2.64E−12 4.23E−10 EHMT1 ENST00000495657 81.41% 35.90% 45.51% ENST00000637318 7.76% 17.69% 9.93% 4.47E−11 6.36E−09 CBWD5 ENST00000441808 11.42% 0.88% 10.54% ENST00000461932 12.77% 23.07% 10.30% 1.66E−10 2.12E−08 NCKAP1L ENST00000547500 21.88% 2.25% 19.62% ENST00000293373 39.72% 77.05% 37.33% 2.62E−10 3.05E−08 SOD2 ENST00000535561 54.55% 4.840% 49.71% ENST00000535459 0.00% 16.75% 16.75% 4.80E−10 5.13E−08 TMSB4X ENST00000380636 20.07% 9.04% 11.02% ENST00000451311 79.93% 90.91% 10.98% 5.20E−10 5.13E−08 ANKRD10 ENST00000460846 20.97% 2.22% 18.75% ENST00000267339 18.95% 43.33% 24.38% 6.04E−10 5.53E−08 FNDC3A ENST00000541916 32.24% 8.21% 24.03% ENST00000398316 10.79% 33.020% 22.23% 2.34E−09 2.00E−07 BAZ2B ENST00000482503 31.03% 2.62% 28.41% ENST00000548440 10.34% 24.67% 14.32% 2.24E−08 1.80E−06 NEDD9 ENST00000504634 25.83% 8.75% 17.09% ENST00000379433 22.50% 63.34% 40.84% 4.19E−08 3.16E−06 CBWD3 ENST00000618921 12.29% 1.65% 10.64% ENST00000614377 11.34% 17.02% 5.67% 1.16E−07 8.27E−06 CARMIL1 ENST00000329474 95.65% 28.84% 66.81% ENST00000461945 4.35% 69.17% 64.82% 1.89E−07 1.28E−05 STK17B ENST00000409228 16.79% 2.00% 14.79% ENST00000449152 11.29% 25.40% 14.11% 3.45E−07 2.21E−05 MED13 ENST00000583958 14.29% 0.34% 13.95% ENST00000397786 85.71% 94.02% 8.31% 7.40E−07 4.34E−05 PECAM1 ENST00000564866 6.94% 0.98% 5.96% ENST00000563924 81.94% 96.17% 14.22% 7.45E−07 4.34E−05 COX14 ENST00000548985 21.74% 0.00% 21.74% ENST00000550487 78.26% 96.27% 18.01% 9.99E−07 5.35E−05 OST4 ENST00000429985 30.16% 7.24% 22.92% ENST00000456793 69.84% 92.76% 22.92% 1.00E−06 5.35E−05 PELI1 ENST00000468869 53.57% 9.09% 44.48% ENST00000358912 32.52% 69.280% 36.76% 1.35E−06 6.92E−05 MZB1 ENST00000503120 48.46% 14.23% 34.230% ENST00000302125 44.61% 84.640% 40.03% 3.14E−06 0.000155 MT2A ENST00000245185 97.71% 80.38% 17.33% ENST00000562017 1.14% 10.61% 9.47% 3.54E−06 0.000168 ATP5ME ENST00000505852 15.38% 1.020% 14.36% ENST00000304312 84.62% 95.90% 11.29% 3.92E−06 0.000179 ST6GAL1 ENST00000448408 85.18% 69.88% 15.30% ENST00000470633 1.18% 4.18% 3.00% 4.41E−06 0.000195 NDUFB11 ENST00000276062 53.33% 19.44% 33.89% ENST00000377811 46.67% 80.56% 33.89% 4.89E−06 0.000209 AFF1 ENST00000511442 59.46% 13.63% 45.83% ENST00000504956 0.00% 28.54% 28.54% 5.73E−06 0.000223 CBWD6 ENST00000617722 35.40% 16.63% 18.77% ENST00000611553 4.67% 17.16% 12.50% 5.63E−06 0.000223 PLCG2 ENST00000567980 11.22% 1.06% 10.17% ENST00000564138 55.44% 76.190% 20.750% 5.74E−06 0.000223 CHD2 ENST00000626782 17.34% 1.62% 15.72% ENST00000635856 0.68% 8.81% 8.13% 5.93E−06 0.000223 ERN1 ENST00000606895 42.92% 26.24% 16.67% ENST00000433197 32.78% 54.610% 21.82% 8.11E−06 0.000297 LMAN1 ENST00000587918 9.59% 4.30% 5.28% ENST00000251047 83.10% 94.57% 11.47% 1.05E−05 0.000375 LINC00861 ENST00000651667 25.00% 0.00% 25.00% ENST00000500989 60.00% 87.78% 27.78% 1.14E−05 0.000389 PRDM1 ENST00000369096 66.15% 46.23% 19.91% ENST00000481163 16.36% 29.27% 12.91% 1.15E−05 0.000389 SEC61B ENST00000481573 31.52% 11.40% 20.12% ENST00000223641 67.96% 88.18% 20.22% 1.45E−05 0.000478 DENND1B ENST00000468589 24.64% 8.04% 16.60% ENST00000620048 7.83% 43.720% 35.89% 1.83E−05 0.000585 TCERG1 ENST00000509810 41.11% 6.69% 34.42% ENST00000506524 25.56% 36.14% 10.58% 2.26E−05 0.000707 AC007780.1 ENST00000592030 42.86% 13.90% 28.95% ENST00000590353 46.43% 84.49% 38.06% 3.62E−05 0.001077 AKAP13 ENST00000560957 16.13% 2.91% 13.22% ENST00000560676 0.36% 10.42% 10.06% 4.08E−05 0.001189 PSMB10 ENST00000570304 26.09% 0.99% 25.10% ENST00000358514 69.56% 92.08% 22.51% 5.67E−05 0.001612 RBM5 ENST00000437500 28.57% 2.36% 26.20% ENST00000492472 0.00% 12.21% 12.21% 5.89E−05 0.001612 GTF2H2 ENST00000521858 24.14% 4.32% 19.81% ENST00000274400 24.14% 38.36% 14.22% 9.73E−05 0.002597 PSMA3 ENST00000554207 32.00% 6.63% 25.37% ENST00000557290 32.80% 70.55% 37.75% 0.0001283 0.003355 GOLGA8N ENST00000569536 31.58% 1.09% 30.49% ENST00000448387 68.42% 95.65% 27.23% 0.0001321 0.003383 SEPTIN6 ENST00000481072 41.36% 19.05% 22.31% ENST00000354416 46.97% 62.83% 15.85% 0.0001807 0.004538 CD74 ENST00000353334 34.54% 21.5% 13.03% ENST00000523836 9.10% 27.27% 18.17% 0.0002092 0.00509 SEC62 ENST00000460513 26.83% 10.50% 16.33% ENST00000337002 19.72% 51.50% 31.78% 0.0002106 0.00509 TAPBP ENST00000475304 15.56% 1.77% 13.79% ENST00000434618 73.33% 93.04% 19.71% 0.0002495 0.005811 CCNL1 ENST00000467849 18.75% 0.00% 18.75% ENST00000474539 38.39% 60.15% 21.76% 0.0003674 0.008116 MIR4435-2HG ENST00000409569 29.16% 9.61% 19.55% ENST00000603310 4.47% 12.450% 7.98% 0.0003869 0.008401 YARS ENST00000478828 31.58% 7.14% 24.44% ENST00000373477 17.08% 60.32% 43.23% 0.0004211 0.008991 SON ENST00000429093 15.91% 1.85% 14.06% ENST00000381679 14.80% 30.30% 15.50% 0.000492 0.010332 USP48 ENST00000527823 13.74% 0.00% 13.74% ENST00000464577 2.20% 12.38% 10.190% 0.0005296 0.010943 RABGAP1L ENST00000486220 21.62% 6.22% 15.40% ENST00000635248 5.41% 29.75% 24.340% 0.0005583 0.011351 GARS-DT ENST00000663944 37.340% 10.96% 26.38% ENST00000656943 3.03% 16.04% 13.01% 0.0005826 0.011662 CWF19L2 ENST00000462890 17.65% 0.000% 17.65% ENST00000282251 82.35% 100.00% 17.65% 0.0006352 0.012519 C18orf32 ENST00000582392 11.11% 0.000% 11.11% ENST00000318240 27.78% 47.49% 19.71% 0.0006872 0.013138 TOMM5 ENST00000540941 23.080% 2.86% 20.22% ENST00000321301 76.92% 90.48% 13.55% 0.0006855 0.013138 CXCR4 ENST00000409817 32.93% 11.37% 21.56% ENST00000241393 67.07% 88.63% 21.56% 0.0007137 0.013401 RBM47 ENST00000510871 33.33% 6.77% 26.56% ENST00000513473 4.17% 27.47% 23.30% 0.0007218 0.013401 PCM1 ENST00000523540 20.00% 2.050% 17.95% ENST00000327578 4.00% 10.93% 6.93% 0.0007561 0.013836 AL354740.1 ENST00000593917 85.11% 56.63% 28.47% ENST00000429998 12.96% 39.70% 26.74% 0.0008305 0.014983 RNF149 ENST00000424632 19.64% 5.34% 14.30% ENST00000295317 38.82% 43.20% 4.38% 0.0011199 0.019652 LNPEP ENST00000395784 24.24% 5.38% 18.87% ENST00000231368 59.69% 81.60% 21.91% 0.0011647 0.019893 UBE2B ENST00000511807 21.62% 2.80% 18.82% ENST00000499038 2.70% 21.190% 18.49% 0.0011612 0.019893 AC139887.2 ENST00000503185 24.000% 2.15% 21.85% ENST00000660016 0.00% 16.49% 16.49% 0.0012154 0.02004 MRPS31 ENST00000498078 19.13% 4.19% 14.94% ENST00000323563 64.80% 86.43% 21.630% 0.0011911 0.02004 SELENOS ENST00000398226 23.86% 11.51% 12.35% ENST00000526049 17.81% 41.20% 23.39% 0.0012203 0.02004 BHLHE40 ENST00000256495 84.38% 51.36% 33.01% ENST00000467610 15.63% 46.65% 31.03% 0.0013279 0.021531 SSR1 ENST00000462112 50.00% 9.72% 40.27% ENST00000244763 20.83% 72.22% 51.39% 0.0013947 0.022111 U2AF2 ENST00000592867 23.81% 2.56% 21.25% ENST00000587196 4.76% 26.50% 21.73% 0.0013981 0.022111 RRBP1 ENST00000468428 52.11% 20.28% 31.82% ENST00000470422 43.37% 73.58% 30.21% 0.0016103 0.025156 EDEM1 ENST00000443790 15.97% 0.81% 15.16% ENST00000256497 71.60% 88.02% 16.430% 0.0016507 0.025476 SMG6 ENST00000572205 37.50% 9.17% 28.33% ENST00000263073 37.50% 75.83% 38.33% 0.0017729 0.027036 IGHA2 ENST00000390539 97.89% 80.56% 17.34% ENST00000497872 2.11% 19.44% 17.340% 0.001836 0.02767 RUNX1 ENST00000300305 76.59% 33.14% 43.45% ENST00000469087 11.90% 27.16% 15.260% 0.0019933 0.029691 SEL1L3 ENST00000513416 25.05% 12.17% 12.88% ENST00000509290 0.00% 15.49% 15.49% 0.0020425 0.030073 MRPS5 ENST00000461916 29.17% 5.26% 23.90% ENST00000482821 0.00% 13.32% 13.320% 0.0022998 0.032734 STK4 ENST00000372806 8.93% 3.67% 5.26% ENST00000488618 57.46% 67.74% 10.28% 0.0022829 0.032734 C5orf56 ENST00000378953 28.05% 13.92% 14.12% ENST00000612967 31.71% 48.33% 16.62% 0.0025365 0.035707 LAMTOR2 ENST00000368304 13.51% 1.40% 12.12% ENST00000368305 83.78% 94.42% 10.63% 0.0025733 0.03583 HDLBP ENST00000460826 21.43% 5.43% 16.00% ENST00000425989 28.57% 51.68% 23.11% 0.0028858 0.039327 PRKCB ENST00000498058 15.79% 2.65% 13.14% ENST00000482000 0.00% 11.26% 11.26% 0.0028758 0.039327 SPDYE1 ENST00000652520 40.54% 17.97% 22.57% ENST00000258704 59.46% 82.03% 22.57% 0.0029923 0.040349 PFDN2 ENST00000468311 17.24% 2.19% 15.05% ENST00000368010 82.76% 97.81% 15.05% 0.0030687 0.040948 DOCK10 ENST00000458608 42.31% 8.52% 33.79% ENST00000489831 11.54% 23.11% 11.57% 0.0032831 0.043358 PLXNC1 ENST00000549187 20.00% 2.44% 17.56% ENST00000258526 37.35% 58.34% 20.99% 0.0035745 0.046252 U2SURP ENST00000488497 38.90% 6.31% 32.59% ENST00000480029 13.73% 32.21% 18.48% 0.0036316 0.046521 FUS ENST00000254108 12.82% 2.26% 10.56% ENST00000487509 48.72% 59.83% 11.11% 0.0040139 0.049921 ZNF518A ENST00000442635 31.58% 3.53% 28.05% ENST00000484770 47.37% 66.42% 19.05% 0.0039841 0.049921

TABLE 12 Fibroblast cell specific DCI genes Prevalance of Prevalance Prevalance Prevalance non-fibroblast difference of of cell Prevalance of Fibroblast fibroblast fibroblast Prevalance preferred of non- cell cell preferred cell preferred difference Non-fibroblast isoform non-fibroblast cell fibroblast preferred isoform in isoform in of fibroblast cell in preferred isoform cell FDR Gene isoform fibroblast non-fibroblast cell preferred preferred isoform fibroblast in non-fibroblast preferred adj Name ID cells cells isoform ID cells calle isoform P-values P-values MT2A ENST00000562017 27.18% 5.55% 21.63% ENST00000245185 56.32% 88.14% 31.82% 3.06E−42 7.22E−39 NBEAL1 ENST00000449802 38.28% 9.49% 28.79% ENST00000460355 35.33% 84.04% 48.710% 3.92E−35 4.62E−32 VAMP8 ENST00000409760 44.44% 1.24% 43.20% ENST00000263864 55.56% 95.36% 39.79% 7.74E−16 4.56E−13 TYMP ENST00000652237 25.00% 0.00% 25.00% ENST00000487162 62.50% 85.42% 22.92% 2.54E−15 1.20E−12 SYNE2 ENST00000441438 37.78% 0.15% 37.63% ENST00000358025 4.58% 11.61% 7.04% 3.69E−15 1.45E−12 S100A10 ENST00000368809 13.04% 0.00% 13.04% ENST00000368811 86.96% 99.36% 12.400% 2.90E−13 9.77E−11 ITPR2 ENST00000536627 33.33% 0.38% 32.96% ENST00000381340 55.56% 85.15% 29.60% 1.40E−11 4.11E−09 FNDC3A ENST00000492622 53.53% 10.01% 43.52% ENST00000398316 6.04% 26.90% 20.86% 1.50E−10 3.93E−08 AKAP13 ENST00000560676 44.88% 7.85% 37.02% ENST00000560340 0.00% 14.45% 14.45% 1.87E−09 4.01E−07 NR4A2 ENST00000339562 96.13% 32.07% 64.06% ENST00000409572 3.85% 62.44% 58.59% 1.78E−09 4.01E−07 AC015712.2 ENST00000560068 47.06% 6.15% 40.90% ENST00000656756 28.32% 93.85% 65.53% 2.39E−09 4.70E−07 GOT1 ENST00000471741 35.71% 2.19% 33.52% ENST00000370508 64.29% 97.81% 33.52% 7.60E−09 1.24E−06 NAMPT ENST00000393618 17.65% 3.32% 14.32% ENST00000486949 8.82% 20.59% 11.77% 7.11E−09 1.24E−06 SERPINB6 ENST00000644828 33.33% 1.33% 32.01% ENST00000644178 33.33% 67.07% 33.74% 7.91E−09 1.24E−06 NUTM2A-AS1 ENST00000433530 25.00% 0.89% 24.11% ENST00000660726 0.00% 8.30% 8.30% 4.10E−08 6.04E−06 PHKA2 ENST00000486231 33.33% 0.00% 33.33% ENST00000379942 55.56% 96.47% 40.92% 5.32E−08 7.38E−06 ARHGAP15 ENST00000548929 31.25% 0.00% 31.250% ENST00000409869 0.00% 19.64% 19.640% 6.32E−08 7.90E−06 C15orf48 ENST00000558632 46.67% 8.83% 37.830% ENST00000396650 36.66% 58.78% 22.11% 6.37E−08 7.90E−06 PAPPA2 ENST00000367661 62.76% 13.68% 49.080% ENST00000367662 33.24% 81.96% 48.72% 7.79E−08 9.18E−06 SASH1 ENST00000637729 20.69% 0.84% 19.85% ENST00000470750 0.00% 17.65% 17.65% 8.94E−08 1.00E−05 ST3GAL6 ENST00000495502 54.55% 2.05% 52.49% ENST00000477899 18.18% 55.46% 37.27% 1.11E−07 1.19E−05 COL4A1 ENST00000647632 13.66% 3.92% 9.74% ENST00000375820 47.70% 64.53% 16.83% 1.34E−07 1.37E−05 Z93930.2 ENST00000458080 50.00% 1.09% 48.91% ENST00000585003 50.00% 89.13% 39.13% 1.55E−07 1.53E−05 RBX1 ENST00000476110 12.500% 0.00% 12.500% ENST00000216225 87.50% 94.44% 6.94% 2.40E−07 2.26E−05 UBE2L3 ENST00000545681 25.00% 0.00% 25.00% ENST00000342192 75.00% 98.42% 23.42% 4.54E−07 4.12E−05 SELENOS ENST00000527833 16.67% 0.42% 16.25% ENST00000534014 8.33% 39.53% 31.19% 7.75E−07 6.77E−05 ERO1A ENST00000556223 21.35% 1.32% 20.03% ENST00000395686 48.24% 74.83% 26.59% 8.96E−07 7.55E−05 ARHGAP26 ENST00000469131 24.49% 0.00% 24.49% ENST00000646213 10.20% 39.66% 29.45% 1.30E−06 0.00011 MYO9A ENST00000569314 20.00% 0.00% 20.00% ENST00000356056 7.49% 30.80% 23.31% 1.51E−06 0.00012 RORA ENST00000561093 21.74% 3.16% 18.58% ENST00000335670 30.43% 52.28% 21.85% 1.60E−06 0.00012 RABGAP1 ENST00000480054 19.81% 1.29% 18.52% ENST00000456584 4.16% 13.88% 9.72% 1.76E−06 0.00013 N4BP2L2 ENST00000512755 54.59% 45.19% 9.41% ENST00000503814 8.08% 26.51% 18.42% 2.81E−06 0.0002 ARL10 ENST00000507151 30.00% 0.000% 30.00% ENST00000310389 20.000% 47.19% 27.19% 3.12E−06 0.00022 FAAP20 ENST00000420964 26.67% 2.01% 24.65% ENST00000378546 0.00% 45.58% 45.58% 3.69E−06 0.00025 CD59 ENST00000528987 44.44% 2.49% 41.96% ENST00000642928 11.11% 67.64% 56.53% 4.36E−06 0.00029 LIMS1 ENST00000434274 31.58% 9.79% 21.79% ENST00000393310 0.48% 21.00% 20.52% 5.42E−06 0.00035 CD44 ENST00000532339 23.08% 0.00% 23.08% ENST00000525209 0.00% 13.69% 13.69% 5.81E−06 0.00035 PDK1 ENST00000466437 10.53% 0.00% 10.53% ENST00000392571 0.00% 7.62% 7.62% 5.75E−06 0.00035 PCNX1 ENST00000554707 25.00% 1.23% 23.77% ENST00000554879 5.00% 18.22% 13.22% 6.77E−06 0.0004 ARHGAP24 ENST00000503917 30.95% 5.44% 25.51% ENST00000509709 4.76% 26.76% 22.00% 7.29E−06 0.00042 MRPS24 ENST00000418740 42.86% 2.83% 40.030% ENST00000317534 57.14% 94.99% 37.85% 7.58E−06 0.00043 COL4A2 ENST00000649101 25.47% 15.90% 9.57% ENST00000650225 9.33% 21.13% 11.80% 9.84E−06 0.00054 ADGRF5 ENST00000265417 82.01% 31.60% 50.41% ENST00000283296 9.99% 60.53% 50.54% 1.75E−05 0.00094 FKBP2 ENST00000449942 16.13% 0.64% 15.49% ENST00000541388 0.00% 9.73% 9.73% 1.94E−05 0.00101 PHKA2-AS1 ENST00000452900 44.44% 1.43% 43.02% ENST00000654006 55.56% 98.57% 43.02% 2.03E−05 0.00104 C1orf56 ENST00000473308 45.45% 4.26% 41.20% ENST00000368926 54.55% 91.49% 36.94% 2.14E−05 0.00107 ABCA6 ENST00000590645 28.03% 0.00% 28.03% ENST00000284425 41.48% 68.83% 27.35% 2.27E−05 0.00111 CREB5 ENST00000396298 30.00% 13.12% 16.88% ENST00000357727 30.00% 62.41% 32.41% 2.70E−05 0.00128 RRBP1 ENST00000620641 21.43% 0.00% 21.43% ENST00000468428 21.43% 35.29% 13.86% 2.72E−05 0.00128 EHMT1 ENST00000637335 10.71% 0.57% 10.14% ENST00000495657 30.060% 66.50% 36.44% 3.05E−05 0.00141 PNN ENST00000554117 5.26% 0.00% 5.26% ENST00000216832 89.47% 99.58% 10.10% 3.16E−05 0.00143 RUNX1 ENST00000358356 37.11% 1.44% 35.67% ENST00000300305 14.290% 52.30% 38.02% 3.31E−05 0.00147 KCP ENST00000616669 28.57% 2.24% 26.33% ENST00000492679 57.14% 86.49% 29.34% 4.12E−05 0.0018 AFF1 ENST00000511996 37.50% 1.41% 36.09% ENST00000511442 0.00% 26.34% 26.34% 4.28E−05 0.00183 PIK3R1 ENST00000518813 33.33% 3.59% 29.75% ENST00000521381 38.20% 61.32% 23.12% 4.49E−05 0.00189 GSK3B ENST00000474830 26.32% 3.05% 23.26% ENST00000264235 47.85% 87.79% 39.94% 4.64E−05 0.00192 TTC3 ENST00000484047 30.43% 2.81% 27.63% ENST00000399017 37.04% 50.52% 13.48% 5.31E−05 0.00216 SMARCB1 ENST00000643421 11.11% 0.45% 10.66% ENST00000644036 66.67% 92.15% 25.49% 6.33E−05 0.00253 YIF1A ENST00000359461 33.33% 12.24% 21.09% ENST00000376901 50.00% 87.75% 37.75% 6.99E−05 0.00275 CYB5A ENST00000583418 19.36% 5.98% 13.38% ENST00000340533 78.66% 91.47% 12.80% 7.14E−05 0.00276 SRP19 ENST00000505459 46.67% 22.66% 24.01% ENST00000445150 0.00% 12.95% 12.95% 7.70E−05 0.00293 TMEM147 ENST00000392204 45.45% 3.31% 42.15% ENST00000222284 54.55% 91.73% 37.19% 7.93E−05 0.00297 SMAD3 ENST00000439724 22.22% 0.00% 22.22% ENST00000327367 55.56% 91.21% 35.65% 8.72E−05 0.00321 NCOA2 ENST00000520416 25.00% 0.00% 25.00% ENST00000452400 53.46% 83.68% 30.22% 8.95E−05 0.00325 SRSF3 ENST00000339436 37.50% 7.03% 30.47% ENST00000373715 20.12% 64.62% 44.50% 9.99E−05 0.00357 GBP1 ENST00000479889 11.48% 0.00% 11.48% ENST00000370473 78.69% 91.91% 13.22% 0.000102 0.0036 TMED10 ENST00000555085 17.65% 3.36% 14.29% ENST00000303575 58.82% 85.90% 27.08% 0.000112 0.00387 CALCOCO2 ENST00000258947 70.59% 20.99% 49.60% ENST00000510356 0.00% 45.51% 45.510% 0.000119 0.00394 MTREX ENST00000508716 36.36% 12.09% 24.27% ENST00000230640 27.13% 73.35% 46.22% 0.000117 0.00394 TNPO1 ENST00000509030 18.75% 1.20% 17.55% ENST00000337273 56.24% 80.84% 24.59% 0.000118 0.00394 RHOA ENST00000265538 33.33% 18.88% 14.45% ENST00000418115 53.33% 71.33% 18.00% 0.000155 0.00499 NOC3L ENST00000461562 45.45% 6.10% 39.36% ENST00000371361 30.68% 78.05% 47.37% 0.000171 0.00545 XRN1 ENST00000467077 33.33% 2.08% 31.250% ENST00000264951 40.00% 53.28% 13.28% 0.000173 0.00545 GTF2H2 ENST00000523003 20.00% 3.17% 16.83% ENST00000521942 12.00% 26.19% 14.19% 0.000204 0.00634 SEC61B ENST00000481573 38.46% 13.42% 25.04% ENST00000223641 61.54% 86.10% 24.56% 0.000209 0.00639 OAS3 ENST00000549918 20.00% 0.00% 20.00% ENST00000548514 0.00% 34.69% 34.69% 0.000225 0.0068 SEC61G ENST00000450622 18.18% 6.25% 11.93% ENST00000395535 77.27% 93.20% 15.940% 0.000231 0.0069 VPS8 ENST00000460158 25.00% 0.00% 25.00% ENST00000485024 0.00% 29.66% 29.66% 0.000235 0.00692 MIR29B2CHG ENST00000637970 12.50% 0.00% 12.50% ENST00000608023 87.50% 96.76% 9.26% 0.00024 0.00699 PPIA ENST00000494484 18.90% 5.05% 13.85% ENST00000468812 78.02% 94.35% 16.33% 0.000253 0.00727 DLC1 ENST00000511869 34.13% 0.00% 34.13% ENST00000276297 42.72% 60.44% 17.72% 0.00026 0.00738 PPA1 ENST00000373230 30.00% 0.54% 29.460% ENST00000610026 20.00% 44.960% 24.96% 0.000272 0.00763 TOMM70 ENST00000483945 44.44% 5.21% 39.24% ENST00000284320 55.56% 88.54% 32.99% 0.000277 0.00767 BOLA2B ENST00000565525 15.38% 0.49% 14.89% ENST00000567436 0.00% 10.84% 10.84% 0.000284 0.0078 AC011447.3 ENST00000592022 18.18% 0.57% 17.61% ENST00000657925 72.73% 94.89% 22.160% 0.000293 0.00786 STX12 ENST00000472285 31.58% 12.500% 19.08% ENST00000373943 34.94% 59.17% 24.22% 0.00029 0.00786 IGFBP7 ENST00000514062 15.97% 5.42% 10.55% ENST00000295666 72.44% 90.25% 17.81% 0.000302 0.00791 KLHL28 ENST00000556239 18.90% 0.00% 18.90% ENST00000396128 37.50% 47.64% 10.14% 0.000314 0.00813 SPATA1 ENST00000490879 57.14% 37.21% 19.93% ENST00000460286 28.57% 49.61% 21.04% 0.000372 0.00943 RGS12 ENST00000502947 25.00% 0.00% 25.00% ENST00000382788 0.00% 44.52% 44.52% 0.000387 0.0097 RHOT1 ENST00000652713 37.50% 0.00% 37.50% ENST00000581031 0.00% 21.13% 21.13% 0.000391 0.00972 FAM91A1 ENST00000519721 19.85% 0.79% 19.05% ENST00000520246 0.00% 12.32% 12.32% 0.000497 0.01221 NUP58 ENST00000495460 16.67% 2.65% 14.02% ENST00000463407 4.17% 22.93% 18.77% 0.000504 0.01225 TAGLN ENST00000533863 42.86% 1.45% 41.41% ENST00000392951 57.14% 95.65% 38.51% 0.00054 0.01299 ANPEP ENST00000558740 40.00% 1.91% 38.09% ENST00000300060 60.00% 88.59% 28.59% 0.000592 0.0141 AOPEP ENST00000473778 54.55% 13.33% 41.21% ENST00000375315 9.09% 28.67% 19.58% 0.000629 0.01484 SNRPF ENST00000549580 27.27% 2.70% 24.57% ENST00000266735 63.64% 94.59% 30.96% 0.000641 0.01496 SMPD4 ENST00000433118 9.52% 1.05% 8.48% ENST00000409031 88.09% 98.95% 10.86% 0.000715 0.0162 PSME4 ENST00000488687 69.23% 41.33% 27.90% ENST00000404125 0.00% 44.78% 44.78% 0.00074 0.01662 PNKD ENST00000483797 11.11% 0.00% 11.11% ENST00000258362 0.00% 5.24% 5.24% 0.000806 0.01776 TNFAIP8 ENST00000504771 45.45% 8.11% 37.35% ENST00000513374 36.360% 70.93% 34.57% 0.000832 0.01817 IFIT3 ENST00000371811 90.38% 50.68% 39.70% ENST00000371818 9.62% 49.32% 39.70% 0.000865 0.01871 MICOS13 ENST00000587950 17.650% 2.170% 15.48% ENST00000309324 82.35% 95.04% 12.69% 0.000962 0.02062 MIS18BP1 ENST00000451174 28.57% 12.57% 16.01% ENST00000469020 0.00% 14.20% 14.20% 0.000971 0.02063 C5orf56 ENST00000378947 31.82% 13.56% 18.25% ENST00000612967 31.82% 45.67% 13.85% 0.001042 0.02175 LAMTOR2 ENST00000368302 15.79% 1.290% 14.500% ENST00000368305 78.95% 93.99% 15.040% 0.001056 0.02184 AC245297.3 ENST00000668239 12.00% 1.17% 10.83% ENST00000619653 65.33% 74.14% 8.81% 0.001118 0.02279 LSM1 ENST00000523511 28.57% 0.000% 28.57% ENST00000311351 28.57% 74.42% 45.85% 0.001121 0.02279 RNF216 ENST00000476345 33.33% 2.27% 31.06% ENST00000425013 33.33% 65.74% 32.410% 0.001154 0.02315 STAT6 ENST00000556155 46.67% 1.46% 45.20% ENST00000557781 23.33% 67.02% 43.69% 0.001169 0.02315 CHCHD3 ENST00000262570 24.03% 6.45% 17.58% ENST00000457942 69.23% 87.14% 17.91% 0.001202 0.02361 DKK3 ENST00000528188 25.00% 0.00% 25.00% ENST00000396505 75.00% 96.08% 21.08% 0.001235 0.02407 ABCE1 ENST00000510321 64.29% 30.85% 33.44% ENST00000296577 2.06% 64.89% 62.83% 0.001372 0.0263 DST ENST00000487754 30.43% 13.93% 16.51% ENST00000340834 18.48% 33.69% 15.21% 0.001437 0.02732 SUPT5H ENST00000598520 72.73% 12.630% 60.10% ENST00000599335 18.18% 46.32% 28.130% 0.001452 0.02739 HECTD1 ENST00000611816 37.50% 4.80% 32.70% ENST00000399332 18.75% 28.57% 9.82% 0.00152 0.02845 DENR ENST00000539463 52.22% 14.84% 37.38% ENST00000537955 0.000% 30.84% 30.84% 0.001598 0.02943 TENM4 ENST00000529798 22.22% 0.00% 22.22% ENST00000278550 66.77% 96.49% 29.73% 0.001597 0.02943 7-Mar ENST00000421037 17.62% 0.00% 17.62% ENST00000259050 0.58% 17.85% 17.27% 0.001761 0.03195 QSOX1 ENST00000367600 43.10% 20.41% 22.69% ENST00000367602 50.00% 78.50% 28.51% 0.001898 0.03391 HNRNPA2B1 ENST00000356674 23.28% 7.28% 16.00% ENST00000463181 18.45% 36.49% 18.05% 0.001963 0.03455 DNAJC10 ENST00000491074 50.00% 3.42% 46.58% ENST00000650903 0.00% 19.44% 19.44% 0.002048 0.03543 H2AFJ ENST00000501744 22.22% 3.35% 18.870% ENST00000544848 77.78% 96.65% 18.87% 0.002063 0.03543 KMT2E ENST00000257745 30.30% 6.02% 24.27% ENST00000476671 26.92% 38.73% 11.80% 0.002073 0.03543 SENP6 ENST00000493959 31.25% 4.05% 27.20% ENST00000424947 6.25% 16.89% 10.64% 0.002051 0.03543 SLC20A1 ENST00000492076 41.43% 16.190% 25.24% ENST00000272542 0.03% 33.870% 33.840% 0.002187 0.0371 HMCN1 ENST00000485744 28.57% 2.29% 26.280% ENST00000271588 71.43% 97.71% 26.28% 0.002213 0.03727 NBPF15 ENST00000577412 20.41% 5.47% 14.95% ENST00000584793 56.86% 81.58% 24.71% 0.002257 0.03775 CBWD5 ENST00000485088 22.58% 7.38% 15.21% ENST00000468198 14.84% 24.38% 9.54% 0.002403 0.03971 UVRAG ENST00000528264 25.00% 0.75% 24.25% ENST00000356136 62.50% 81.09% 18.59% 0.002408 0.03971 SNAPC3 ENST00000461041 25.00% 3.57% 21.43% ENST00000380821 28.66% 76.79% 48.13% 0.002556 0.04185 ZNF431 ENST00000598331 28.57% 6.32% 22.26% ENST00000311048 57.39% 90.52% 33.13% 0.002607 0.04239 DLD ENST00000489184 14.29% 0.00% 14.29% ENST00000205402 35.71% 58.55% 22.84% 0.002766 0.04438 ATAD2B ENST00000474583 42.86% 4.900% 37.96% ENST00000238789 16.06% 54.19% 38.13% 0.002983 0.04739 SYNE1 ENST00000409694 28.29% 2.50% 25.80% ENST00000478916 27.35% 60.49% 33.14% 0.002994 0.04739 IRF2 ENST00000509274 37.50% 1.65% 35.85% ENST00000512020 0.00% 27.95% 27.95% 0.003143 0.0494 RIC1 ENST00000251879 32.50% 9.23% 23.27% ENST00000276898 38.75% 85.51% 46.75% 0.003194 0.04987

TABLE 13 Consensus filtering of RCC1 variants Minimal cellular prevalence across all cells SNV counts past 0% (1 0.1% (3 0.2% (6 0.4% (13 0.8% (27 1% (34 5% (173 filtering cell) cells) cells) cells) cells) cells) cells) Minimal 1 3,001,137 249,149 86,212 26,895 9,146 6,632 1,004 consensus 2 2,801,998 240,527 83,188 25,939 8,805 6,390* 980 reads 3 556,867 43,562 15,399 5,960 2,951 2,399 568 supporting 4 121,169 11,979 5,548 2,855 1,678 1,382 383 5 35,991 6,464 3,424 1,948 1,124 954 296 6 17,311 4,639 2,634 1,472 870 739 245 *default

TABLE 14 Expanded mutations in tumor cells REF_tumor REF_normal ALT_tumor ALT_normal FDR diff_ALT_cellular cell cell cell cell adj prevalence_tumor vs CHR POS REF ALT numbers numbers numbers numbers P-values normal chr2 8.8E+07 T G 21 41 142 17 3.33E−15 0.578062196 chr6 3.1E+07 A T, C 47 171 80 30 2.09E−17 0.480667528 chr6 3.1E+07 C T 31 156 303 117 3.77E−34 0.4786142 chr6 3.1E+07 T C 29 155 311 120 4.25E−35 0.478342246 chr6 3.1E+07 C G 40 155 295 110 4.90E−31 0.465502675 chr3 1.5E+07 G A 13 40 275 39 1.54E−22 0.461190225 chr17 8376238 A T 3 14 88 15 9.78E−08 0.449791588 chr7 1.4E+08 G A 355 240 1014 101 2.75E−50 0.444498949 chr2 1.4E+07 G A 410 158 885 50 8.46E−32 0.443013068 chr6 3.1E+07 C T 41 152 286 117 8.29E−28 0.439673499 chr2 1.4E+07 G A, T 496 176 817 40 1.28E−30 0.437053962 chr6 3.1E+07 T C 69 172 241 92 3.90E−23 0.428934506 chr6 3.1E+07 C T 36 145 309 129 2.17E−28 0.424849254 KI270467.1 3371 C T, A 464 148 708 33 3.55E−24 0.421775121 KI270467.1 2940 A G, C 286 90 792 41 7.83E−21 0.421716778 chr8 4.2E+07 C T 259 68 487 21 9.56E−13 0.416859957 KI270467.1 3002 T C 420 106 684 27 2.97E−18 0.416557699 KI270467.1 3210 T A, G 384 124 787 43 6.40E−23 0.41459012 KI270467.1 2905 A T, G 396 112 715 34 1.61E−19 0.410687644 KI270467.1 2420 T A 303 155 937 83 8.53E−33 0.406905665 chr7 1.3E+08 A G, C 308 275 973 151 1.98E−49 0.405102748 KI270467.1 2835 T G, A 317 108 801 49 8.88E−22 0.40435605 chr3 3.8E+07 C T 13 54 254 66 7.09E−20 0.401310861 KI270467.1 2382 G T, A 302 154 938 85 4.86E−32 0.400803077 chr2 1.4E+07 C T 336 131 966 68 1.42E−27 0.400226941 KI270467.1 2279 A G 323 167 918 88 8.46E−32 0.394627988 KI270467.1 2820 G A, T, C 298 102 814 52 6.12E−21 0.394352051 KI270467.1 2074 A G, T 380 181 854 77 4.86E−30 0.393608734 chr2 1.4E+07 G A 506 153 792 43 1.03E−22 0.390781736 KI270467.1 2217 T A, G 396 177 840 72 1.71E−28 0.390455024 KI270467.1 2472 A G 433 174 799 61 2.32E−26 0.388964493 chr17 1.8E+07 G A 6 20 85 24 2.70E−06 0.388611389 KI270467.1 3221 C A 410 121 762 43 1.52E−19 0.387975527 KI270467.1 2253 C A, G 324 161 901 86 9.50E−30 0.387332066 KI270467.1 2898 G C, T, A 415 119 698 38 3.64E−18 0.385095656 KI270467.1 3082 G A 389 98 734 36 2.72E−16 0.384949695 chr6 3E+07 C G 14 55 158 63 1.76E−12 0.384706346 KI270467.1 3743 G A, T 451 91 568 19 5.70E−13 0.384681952 chr17 7626829 G C 8 44 198 60 1.04E−15 0.384241972 KI270467.1 2576 A T 421 163 804 61 6.17E−25 0.384005102 KI270467.1 2525 A C, T 469 182 755 56 3.21E−25 0.381535948 chr2 1.4E+07 G A 502 127 772 37 1.60E−18 0.380355707 KI270467.1 3368 T A 519 151 659 33 6.77E−20 0.380074924 KI270467.1 2841 A G 431 117 688 36 3.36E−17 0.379540556 chr6 3.1E+07 G C 40 112 257 107 1.31E−18 0.37673539 KI270467.1 2594 T A 446 160 771 56 1.09E−22 0.374265802 KI270467.1 3306 T C, G, A 424 132 743 47 2.16E−19 0.374105403 KI270467.1 2245 T C, A 424 180 812 71 5.80E−26 0.374089403 KI270467.1 1964 T G, A 341 120 823 60 7.86E−21 0.37371134 chr6 3E+07 T C 13 53 117 59 2.47E−09 0.373214286 KI270467.1 3252 G A 339 114 847 59 2.09E−20 0.373124799 KI270467.1 2927 T C, A 462 104 617 26 3.62E−14 0.371825765 chr3 3.4E+07 C T 8 21 85 25 1.16E−05 0.370500234 KI270467.1 3125 C T, A 404 99 709 36 5.04E−15 0.370350404 KI270467.1 3161 G A, C 349 98 776 46 1.05E−16 0.370333333 KI270467.1 2868 G A 210 81 882 63 8.32E−21 0.370192308 KI270467.1 3404 G T 466 144 709 44 2.27E−19 0.369361702 chr7 1.3E+08 A G 531 334 748 92 5.94E−37 0.368869459 KI270467.1 2292 T C 334 165 910 95 8.12E−28 0.366126639 KI270467.1 2338 C T 162 122 1079 124 1.76E−37 0.365395072 KI270467.1 2979 T C 481 111 644 29 1.19E−14 0.365301587 KI270467.1 2191 A C 573 213 671 45 1.53E−24 0.364970463 KI270467.1 3098 C T 362 84 755 38 6.27E−14 0.364442227 KI270467.1 3344 C G, T 503 133 661 34 6.29E−17 0.364276601 KI270467.1 3183 G T, C 420 104 703 37 6.02E−15 0.363590433 chr3 7E+07 C T 5 14 190 22 4.65E−11 0.363247863 KI270467.1 2442 G A 553 194 679 45 9.92E−23 0.362851845 KI270467.1 2165 A G 276 148 966 106 8.46E−29 0.360454943 KI270467.1 2296 T C, G 342 162 913 94 1.39E−26 0.36030254 chr6 4.4E+07 C T 403 300 621 98 9.97E−32 0.360214157 KI270467.1 2920 C T, G 337 81 728 39 2.30E−13 0.358568075 KI270467.1 2080 T C 400 177 841 83 4.33E−25 0.358448522 KI270467.1 2012 T C 265 118 930 86 6.12E−24 0.35667405 KI270467.1 2624 C A, T, G 223 105 977 89 4.30E−25 0.35540378 KI270467.1 3158 T A, C 584 130 545 19 8.80E−15 0.355211299 chr6 1.6E+08 G A 5 15 237 25 1.43E−13 0.354338843 chr7 1.4E+08 C T 225 175 1148 163 4.37E−40 0.353876752 chr7 1.4E+08 C T 485 226 874 92 2.71E−28 0.353811765 KI270467.1 2284 A G 341 162 903 96 9.16E−26 0.353791221 KI270467.1 3109 C T, A 314 82 795 47 1.81E−14 0.352520953 chrX 7.4E+07 T C 10 50 327 81 3.35E−22 0.352005799 KI270467.1 2629 G T, A 239 110 966 90 2.35E−24 0.351659751 chr7 1.4E+08 T C 237 183 1139 167 2.03E−39 0.350618771 KI270467.1 3049 T G, A 407 95 679 36 4.52E−13 0.350421042 KI270467.1 1950 T C 434 127 731 49 1.05E−16 0.34905872 chr1 2.4E+08 A G 174 44 224 12 1.78E−05 0.348528356 KI270467.1 3184 G C, A, T 588 124 531 18 1.15E−13 0.347770268 chr6 3.1E+07 G C 86 287 109 77 4.25E−15 0.347435897 chr6 3.1E+07 T C 85 281 110 78 7.58E−15 0.346832369 KI270467.1 2955 A C 444 97 634 31 2.97E−12 0.34593866 chr4 1.9E+08 C T 446 82 526 20 8.22E−10 0.345073832 KI270467.1 1961 T C 375 116 776 57 1.05E−16 0.344716582 KI270467.1 2512 A T, G, C 654 213 584 31 1.54E−21 0.344679414 KI270467.1 2921 A G, C 287 82 800 53 1.04E−14 0.343377969 KI270467.1 3301 G A, T 499 132 677 40 1.53E−15 0.343122133 chr2 1.4E+07 G A, C 397 79 776 37 9.08E−12 0.34258606 KI270467.1 2339 G A, T 503 172 717 56 1.42E−19 0.342090883 KI270467.1 3300 G T, C 476 123 677 40 8.91E−15 0.341765147 KI270467.1 3180 T C, A 523 115 601 28 6.85E−13 0.338893313 KI270467.1 3436 G A, T 549 139 609 32 4.99E−15 0.338772233 KI270467.1 2171 T A 270 141 971 113 8.00E−26 0.337551632 KI270467.1 2622 C T 154 94 1050 108 8.12E−29 0.337439558 KI270467.1 3329 C T, G, A 475 124 684 42 1.19E−14 0.337151886 KI270467.1 2996 G A, T, C 243 74 864 59 1.99E−15 0.336878782 KI270467.1 2048 G T, C 260 125 951 102 1.19E−23 0.335962197 KI270467.1 2449 T G, A 617 187 610 36 1.05E−18 0.335712537 KI270467.1 2175 G T 329 153 912 102 2.19E−23 0.334891217 KI270467.1 3134 G T, A 570 123 551 23 7.26E−13 0.333991177 KI270467.1 2034 A G 256 133 969 112 4.30E−25 0.333877551 KI270467.1 3257 A T 545 134 618 33 2.43E−14 0.33377956 KI270467.1 3309 T C, G 481 125 676 42 2.43E−14 0.332772657 KI270467.1 2287 C T, G 227 131 1008 123 3.55E−27 0.331942363 chr3 4984576 A G 8 26 167 43 8.60E−10 0.331097308 KI270467.1 3464 G T 613 143 520 21 3.25E−14 0.330909737 KI270467.1 2610 G T, A 503 153 705 52 9.40E−17 0.329950735 KI270467.1 1954 A G, T 388 116 776 59 2.04E−15 0.32952381 KI270467.1 2294 T C, A 266 134 967 112 3.61E−24 0.328981465 chr11 6.6E+07 C T 337 640 1107 499 1.39E−61 0.328516899 KI270467.1 2121 A C, G 190 122 1045 131 4.35E−29 0.328367285 KI270467.1 1966 T G, A 512 134 647 40 2.19E−14 0.328354804 chr2 1.4E+07 C T 195 97 1120 107 5.53E−26 0.327201223 KI270467.1 2152 T C, G 703 227 545 28 9.47E−21 0.326894796 KI270467.1 3243 A G, C 635 149 553 24 1.58E−14 0.326759892 KI270467.1 2133 T C, G, A 674 209 564 32 5.65E−19 0.322793423 KI270467.1 2025 G T, A 262 116 945 99 5.95E−21 0.322467775 chr11 6.5E+07 C T 507 355 838 153 1.02E−32 0.321867225 chr11 6.5E+07 G A 446 396 935 219 3.51E−38 0.320948058 KI270467.1 2565 G A 157 97 1064 119 1.31E−27 0.320490945 KI270467.1 2015 T C, G 262 111 937 95 5.63E−20 0.320319522 KI270467.1 1945 C G 505 116 622 35 4.03E−12 0.32011964 KI270467.1 3078 T C 602 120 527 21 1.80E−11 0.317848595 chr11 6.5E+07 A G 601 437 795 147 1.82E−35 0.317771912 chr11 6.5E+07 A G 658 460 736 123 9.58E−36 0.316999343 chr6 1.6E+08 T C 8 14 234 26 4.89E−10 0.316942149 KI270467.1 3061 A T 457 94 636 34 2.47E−10 0.316259721 chr17 7626953 C T 20 55 239 85 5.87E−13 0.315637066 chr3 4979718 C T 29 63 290 92 1.81E−14 0.315542522 KI270467.1 3418 C T 591 144 573 31 1.74E−13 0.315125184 KI270467.1 3448 G C 661 148 496 19 1.89E−13 0.314922446 KI270467.1 2626 T A, C 595 166 617 40 2.06E−15 0.31490115 KI270467.1 3074 T A, G 587 117 540 23 3.97E−11 0.314862467 chr9 1.4E+08 T C 9 20 66 26 0.001296 0.314782609 KI270467.1 3176 A G, T, C 444 99 670 40 5.04E−11 0.313666482 KI270467.1 2656 A G, T 224 92 958 91 6.85E−19 0.313222934 KI270467.1 3302 G T, A 579 140 596 34 4.17E−13 0.311831744 KI270467.1 3132 T G, C 604 122 524 22 2.88E−11 0.311761229 KI270467.1 3122 A G, T, C 618 123 493 19 3.89E−11 0.309941558 KI270467.1 3512 A T 646 142 492 20 1.47E−12 0.308880644 KI270467.1 3293 T C, G 478 126 707 51 3.84E−13 0.308488879 chr8 1.3E+08 A G 20 48 157 66 3.48E−08 0.308058281 KI270467.1 3761 C G, T 502 79 473 17 1.44E−07 0.308044872 KI270467.1 2375 C T, A, G 621 201 621 48 2.81E−17 0.307228916 KI270467.1 2791 A T, C 505 97 516 24 3.46E−09 0.307039768 chr7 1.3E+08 G A 319 234 955 186 6.42E−29 0.306750392 chr3 7E+07 G A 2 8 137 17 5.72E−07 0.305611511 KI270467.1 2908 C T, A, G 545 112 557 28 2.16E−10 0.305444646 chr12 9.7E+07 T C 233 37 267 11 0.000699 0.304833333 chr1 1.7E+08 C T 215 52 241 15 4.80E−05 0.304628175 KI270467.1 2170 T C 635 204 606 46 4.14E−17 0.304315874 chr11 6.5E+07 C T 581 419 779 154 7.89E−32 0.30403321 KI270467.1 2168 T C 684 216 560 37 1.16E−17 0.303915712 KI270467.1 1927 C A 226 67 860 64 6.05E−13 0.303347251 KI270467.1 3073 G C, A 648 120 469 16 2.05E−10 0.302227605 KI270467.1 2137 C T, A 688 223 556 38 7.10E−18 0.301351468 chr11 6.5E+07 T C 177 297 1251 403 9.16E−52 0.300336134 KI270467.1 3129 A C, T, G 565 108 537 25 1.30E−09 0.299325901 KI270467.1 3682 A T, G, C 569 102 477 19 6.41E−09 0.298998151 KI270467.1 2033 T G, C 617 193 604 47 6.57E−16 0.298843161 KI270467.1 2354 T G 661 211 581 43 6.78E−17 0.298502542 KI270467.1 2161 T A, G 555 163 643 51 2.77E−14 0.298410123 KI270467.1 3181 T C 690 134 445 14 3.27E−11 0.29747589 KI270467.1 2762 A G 541 108 520 26 1.98E−09 0.296073825 KI270467.1 3776 T A, G 406 68 578 28 4.62E−07 0.295731707 KI270467.1 2007 G A, T 254 107 947 104 6.72E−18 0.29561858 KI270467.1 2177 A G 234 118 994 125 6.43E−21 0.295042962 KI270467.1 3111 T C, A, G 698 123 421 11 3.12E−10 0.294139223 KI270467.1 2436 T C, A 328 130 903 102 4.19E−17 0.293894787 KI270467.1 3388 A C, G 531 133 649 46 7.06E−12 0.29301676 KI270467.1 3731 A T 492 87 539 26 6.73E−08 0.292704909 KI270467.1 2504 G T, A 785 218 457 18 6.83E−17 0.291683725 KI270467.1 2305 C A, G, T 739 224 512 30 5.73E−17 0.291162346 chr11 6.5E+07 T A 531 394 840 187 1.23E−29 0.290832602 KI270467.1 2544 A C, G, T 158 89 1047 122 1.32E−22 0.290680616 KI270467.1 3100 G A 598 104 506 21 1.05E−08 0.290333333 KI270467.1 3194 G T, A 684 128 445 15 2.80E−10 0.289259014 KI270467.1 3113 C G 651 113 456 16 4.09E−09 0.287893111 KI270467.1 2774 A G 534 105 518 27 8.18E−09 0.287849983 KI270466.1 997 C T 282 49 470 25 2.33E−05 0.287162162 KI270467.1 3096 C A 558 98 562 27 2.52E−08 0.285785714 KI270467.1 3469 G T, A, C 697 150 450 18 1.47E−11 0.285184955 KI270467.1 2793 A T 510 98 512 27 3.27E−08 0.284978474 KI270467.1 1957 C T, A 173 71 966 92 2.28E−16 0.283695201 chr6 3.1E+07 G A 618 98 497 19 6.66E−08 0.283346748 chr11 6.5E+07 A G 453 376 930 240 4.83E−30 0.282840803 KI270467.1 3543 C T, A 632 125 478 22 1.35E−09 0.280970767 KI270467.1 3848 T C, G 333 65 651 40 2.60E−07 0.280632985 KI270467.1 2767 A T 514 104 546 32 1.56E−08 0.279800222 KI270467.1 3890 C A 298 41 545 24 0.000119 0.277269824 KI270467.1 2780 C T, A 428 83 603 37 1.44E−07 0.276535726 KI270467.1 2471 T C 749 211 485 28 8.80E−15 0.275875982 chr9 3.6E+07 A G 9 23 85 39 0.000515 0.275223061 chr6 3E+07 C T 17 57 392 123 1.81E−18 0.275101874 KI270467.1 1873 T C, A 362 46 581 24 9.45E−05 0.273261627 KI270467.1 1175 C T 258 27 411 14 0.005654 0.272886361 KI270467.1 2138 C G, T 625 188 602 53 2.99E−13 0.270710534 chr11 6.5E+07 A G 668 429 712 140 1.06E−25 0.269896335 chr6 4031764 A G 13 49 230 103 3.37E−11 0.268870479 chr11 6.5E+07 T C, A, G 680 413 654 119 4.64E−24 0.266570662 chr11 6.5E+07 G A 622 359 666 120 9.23E−22 0.266558825 KI2704671 2450 T C, G, A 757 205 477 28 1.24E−13 0.266376138 KI2704671 3460 A T, C 691 150 457 23 3.11E−10 0.265135647 chr12 9.7E+07 A G 244 35 264 12 0.005312 0.26436589 chr11 6.6E+07 C T 156 421 1294 711 3.95E−54 0.26432192 KI270467.1 3537 A G 706 141 418 17 1.12E−09 0.264291184 KI270467.1 2760 T A 585 110 477 25 8.82E−08 0.263967357 KI270467.1 2315 T G, C 712 219 546 45 4.04E−14 0.263567712 chr6 3.3E+07 C A 19 56 111 81 2.94E−05 0.262605278 KI270467.1 3519 C T, A 611 116 479 25 4.81E−08 0.262144577 chr6 3.1E+07 C A 76 39 1032 79 2.41E−18 0.261916417 KI270466.1 407 A G, T 228 73 801 78 1.41E−10 0.261869365 KI270467.1 3259 G C, T 592 126 566 37 6.53E−09 0.261779883 KI270467.1 1933 G A, T 207 61 897 75 8.54E−11 0.261029412 KI270467.1 1932 G A, C 461 91 628 42 2.01E−07 0.260886376 chr6 3E+07 A T 254 267 345 123 3.07E−14 0.260575318 KI270467.1 2298 T C, A 723 219 538 44 9.85E−14 0.259345139 chr7 1.3E+08 A G 832 390 448 39 7.45E−23 0.259090909 chr7 1.3E+08 A G, T 784 374 495 55 2.45E−21 0.258815982 KI270467.1 1928 C A 485 92 606 39 4.02E−07 0.257743789 chr11 6.5E+07 C T 561 338 739 153 1.99E−20 0.256852577 KI270467.1 2863 A G 696 130 413 17 1.41E−08 0.256761316 KI270467.1 3351 G A, C, T 770 159 410 16 2.30E−10 0.256029056 chrX 7.4E+07 A C 10 26 364 66 1.93E−14 0.255870728 chrX 7.4E+07 A T 5 18 162 45 3.42E−07 0.255774166 KI270466.1 424 C T 239 77 799 82 3.18E−10 0.254026248 KI270467.1 3364 C G, A 162 71 1008 110 2.04E−15 0.253803655 KI270467.1 3811 G A, C 305 53 657 40 1.36E−05 0.252844656 KI270467.1 2351 A T, G 799 229 452 28 7.65E−14 0.252361535 chr4 2.1E+07 A G 372 64 495 30 4.37E−05 0.25178532 chrX 7.4E+07 T C 103 139 960 260 2.31E−28 0.251475349 chr6 3.1E+07 G T, C 163 446 142 122 4.15E−13 0.250785038 KI270467.1 3203 A G 739 134 410 16 1.66E−08 0.250165361 chr11 6.5E+07 C T, A 399 177 649 104 1.76E−12 0.249168048 KI270467.1 1943 T C 642 124 491 28 7.47E−08 0.249152227 chr7 1.3E+08 A G 667 320 588 90 2.26E−17 0.249013701 chr19 5.1E+07 G A 9 11 107 23 0.003693 0.245943205 KI270466.1 691 T A 244 51 610 45 1.30E−05 0.245535714 chr3 2.8E+07 A T, C 17 25 122 43 0.000567 0.245344901 chr6 2.6E+07 T C 143 22 230 13 0.040157 0.245193412 KI270467.1 2392 C T 89 76 1156 164 4.76E−26 0.245180723 chr6 3.1E+07 G A 1 3 198 9 6.58E−06 0.244974874 chr6 4.4E+07 C T 630 165 712 66 1.32E−10 0.24483713 chr6 3E+07 G A 12 52 443 140 2.41E−19 0.244459707 chr3 4984323 A G 9 19 107 40 0.000539 0.244447691 KI2704671 3719 G T 644 99 399 16 3.80E−06 0.243419901 chr8 4.2E+07 C T 157 32 321 24 0.003383 0.242976689 KI270467.1 2757 G A, C 472 89 582 40 3.12E−06 0.242104644 chr19 8364724 G A 343 55 354 20 0.000792 0.241224295 chr6 3E+07 G A 168 183 391 155 1.82E−11 0.240883446 chr4 2.2E+07 A T 243 23 340 12 0.043586 0.240333252 KI270466.1 1096 C T 323 44 365 18 0.002926 0.240200675 KI270467.1 2821 A G 737 141 382 16 2.55E−08 0.239465401 KI270467.1 3224 T C, G 814 155 366 12 3.00E−09 0.238313204 KI270467.1 1366 C T 154 19 601 24 0.00292 0.237886955 chr8 1.5E+08 A G 147 18 476 20 0.010845 0.237729154 KI270467.1 3330 A T, C 756 153 413 20 8.56E−09 0.237686477 chr6 3E+07 C A 7 21 144 53 1.06E−05 0.237426168 KI270467.1 3361 G C, A 772 161 403 19 3.08E−09 0.237423168 chr6 3E+07 A G 18 54 434 141 1.08E−16 0.237100068 chr4 2.1E+07 A G 415 62 413 22 0.000397 0.236887509 KI270467.1 2910 A G, C 529 103 576 41 1.48E−06 0.236544746 KI270467.1 3491 G A, C 677 139 476 30 7.37E−08 0.235321287 KI270467.1 1905 G C, T 466 66 548 29 0.000141 0.235170767 KI270467.1 1910 T C 476 77 562 34 3.28E−05 0.235119513 chrX 7.4E+07 T C 108 53 134 25 0.00336 0.233206188 chr6 3.7E+07 T C 21 43 139 75 7.66E−05 0.23315678 KI270466.1 649 T G, C 222 51 639 53 1.04E−05 0.232544894 chr6 3.3E+07 C G 21 54 108 83 0.000356 0.231369886 chr11 6.5E+07 A G 610 232 513 68 1.33E−11 0.230145444 KI270467.1 3631 T C, G 423 75 620 43 2.32E−05 0.230032338 KI270467.1 3369 T G 787 167 391 19 4.31E−09 0.229767968 chr7 1.3E+08 A G 834 378 445 51 7.31E−18 0.22904695 KI270467.1 1148 G A 205 23 519 22 0.011248 0.22796194 chr15 3.3E+07 C G 363 151 551 91 5.02E−09 0.226811581 chr4 2.1E+07 T C 371 62 542 36 0.000202 0.226300378 chr11 6.5E+07 G A 431 496 964 433 2.11E−25 0.224946854 chrX 7.4E+07 G A 547 317 733 169 1.02E−15 0.224919624 chrX 7.4E+07 T C 11 33 300 94 6.20E−11 0.224472745 chr5 1.4E+08 A G 445 69 644 40 7.93E−05 0.224395751 chr6 3.3E+07 A G 28 40 173 70 7.25E−05 0.224332881 KI270467.1 2470 A C, T 853 209 377 19 8.01E−11 0.223170732 chrX 7.4E+07 T C 118 63 163 35 0.001455 0.222928317 KI270467.1 3406 C G, A, T 787 165 380 19 1.54E−08 0.222360382 KI2704671 3913 C T 187 23 513 24 0.010221 0.222218845 KI270466.1 976 C T, A 353 49 340 18 0.004801 0.221963774 chr18 5.9E+07 T C 366 216 300 64 2.91E−09 0.221879022 KI270467.1 3657 C A, T, G 654 83 334 11 0.000142 0.221035404 KI270467.1 1853 T A, C 439 43 425 16 0.009145 0.220711707 KI270467.1 2221 C G, A 742 201 481 42 1.12E−09 0.22045567 KI270466.1 461 C G, T 659 139 349 20 4.65E−07 0.220443995 chr4 2.1E+07 A T 374 69 557 42 0.000115 0.219903039 chr6 3.3E+07 G C 5 16 100 44 0.000893 0.219047619 KI270467.1 1915 T C, A 471 74 566 36 0.000151 0.21853248 KI270467.1 3323 A G 756 156 419 25 9.68E−08 0.218474198 KI270467.1 2804 G C, A 737 136 371 18 6.75E−07 0.217954428 chr7 1.3E+08 T A, C, G 816 343 421 48 4.18E−15 0.217577383 KI270467.1 2620 T G, C, A 765 174 439 30 1.97E−08 0.217559117 KI270467.1 1906 T A 535 79 489 28 0.000229 0.21585682 KI270467.1 3695 G T, A 536 83 509 31 0.000144 0.215151515 chr11 6.5E+07 G A, C 696 356 635 127 9.09E−15 0.21414494 chr11 6.5E+07 C T 406 167 652 113 2.94E−09 0.21268566 chr3 8.2E+07 G A 7 4 175 12 0.015726 0.211538462 chrX 7.4E+07 G A 575 358 644 166 1.33E−14 0.211507994 chr2 3.5E+07 A C 276 35 356 19 0.022456 0.211439287 chr8 4.2E+07 G A 356 64 413 31 0.001079 0.210745329 KI270467.1 3335 A G, T 149 61 1023 120 1.25E−11 0.209883469 KI270466.1 294 G A 510 103 489 40 3.24E−05 0.20976921 chr19 8364224 G A 423 186 567 106 5.05E−09 0.209713574 KI270467.1 3619 T C 579 97 477 31 7.39E−05 0.209517045 chr8 1.3E+08 G A, C 469 68 525 32 0.000752 0.208169014 chr9 7.2E+07 G A 21 47 80 66 0.010146 0.208008411 KI270467.1 1981 T C 831 182 359 19 1.93E−08 0.207153309 chr11 295527 T C 104 47 403 67 4.58E−05 0.207152497 KI270466.1 298 T C 545 108 467 37 3.53E−05 0.206290037 KI270466.1 595 A C, G 532 99 426 31 9.29E−05 0.206214871 KI270467.1 3284 T A, C 713 137 466 32 3.16E−06 0.2059011 chrX 7.8E+07 C G 5 16 106 48 0.001051 0.204954955 KI270466.1 326 T A, C 626 112 375 23 3.91E−05 0.204255004 chr11 6.6E+07 T C, G, A 589 343 456 104 2.22E−12 0.203701444 chrX 7.4E+07 A G 6 17 199 56 2.19E−06 0.20360842 chr11 295445 A C 102 43 340 56 0.00043 0.203574204 chr9 1.4E+08 A G 9 20 125 54 0.000827 0.203106091 KI270467.1 2795 G A 729 127 374 20 9.29E−06 0.203020828 chr8 1.3E+08 G A 240 43 758 54 0.00017 0.202818007 chr4 2.1E+07 A G 355 63 587 46 0.000525 0.201123902 chr6 3E+07 C T 59 67 401 137 2.72E−08 0.200170503 KI270466.1 554 T C 364 89 648 70 1.92E−05 0.200064633 KI270466.1 247 T A, C 579 98 339 20 0.000205 0.19978952 chr11 6.6E+07 C T 102 283 1332 764 7.64E−39 0.199166377 KI270466.1 1129 T C 324 42 293 16 0.028336 0.199016375 KI270467.1 1987 C T, G, A 780 165 392 26 5.66E−07 0.198345335 KI270467.1 1956 A G 759 144 402 25 3.66E−06 0.198324236 KI270467.1 1119 C T, A 173 21 578 28 0.016112 0.198211908 KI270467.1 3022 T C, G, A 854 128 251 4 2.24E−06 0.196846291 KI270467.1 3782 G A, C 311 47 660 44 0.001544 0.196195154 chr11 6.5E+07 A G 892 473 488 89 4.80E−16 0.195260199 chr4 1.9E+08 G A 315 54 671 51 0.00065 0.194813098 KI270466.1 290 G A 613 116 381 27 7.03E−05 0.19448861 chr6 3.3E+07 A G 2 14 109 52 0.000318 0.194103194 chr8 1.3E+08 T C 511 63 438 23 0.004687 0.194096601 chr6 3.1E+07 T C 56 207 762 582 9.08E−24 0.193897757 chrX 7.4E+07 A G, C 513 278 633 156 1.45E−10 0.192909016 chr6 3E+07 T G 12 20 136 53 0.00178 0.192891522 chr11 295670 G A 118 41 289 44 0.0052 0.192426651 chr6 3.1E+07 C T 61 199 653 521 3.95E−19 0.190954715 KI270467.1 3735 T A, C 686 99 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0.181091954 chr6 3.1E+07 G A 104 246 728 560 2.61E−17 0.180210918 chr7 1.4E+08 G T 453 105 677 76 6.86E−05 0.179225541 chr11 6.8E+07 T C 241 79 488 76 0.000273 0.17908757 chr6 3.3E+07 C G 2 13 109 53 0.000779 0.178951679 chr6 2888837 A C 3 11 210 46 3.62E−06 0.178897949 chr6 3E+07 C T 19 23 138 54 0.009197 0.17768219 chr6 2889003 T C 5 10 156 38 0.00077 0.177277433 chr6 3E+07 C G 17 35 378 124 3.38E−09 0.177087811 chrX 5.6E+07 C G 4 8 216 33 4.14E−05 0.176940133 chr11 6.8E+07 T G 232 78 500 80 0.000256 0.176730995 chr4 2.1E+07 T C, A 506 65 215 9 0.01125 0.176575327 chr2 3.5E+07 A G 538 59 182 5 0.014926 0.174652778 chr6 3.1E+07 C G 74 199 666 527 4.40E−16 0.174104683 chr6 3.1E+07 C G 50 174 687 546 2.16E−18 0.173824062 chr6 3.3E+07 T C 5 13 98 46 0.01102 0.171795294 chr6 3.1E+07 G T, C 51 176 712 562 1.05E−18 0.171640969 chr6 3.3E+07 C T 2 11 107 47 0.001774 0.171306549 chr6 3E+07 A G 26 40 363 128 2.22E−07 0.171257192 KI270467.1 3786 T C 645 78 347 17 0.006406 0.170851019 chr8 1.3E+08 C G 160 42 959 92 6.80E−06 0.170448028 chr6 3.1E+07 A C, T 57 181 691 553 2.09E−17 0.170390797 chr19 1.8E+07 G T 142 77 272 73 0.002223 0.170338164 chr6 3.1E+07 A T 53 186 774 608 3.28E−20 0.170169865 KI270467.1 1936 T A, C 690 113 430 31 0.000748 0.168650794 chr6 3E+07 C A 19 32 357 114 1.55E−07 0.168646167 chr6 3.1E+07 T C 61 195 770 611 3.92E−19 0.168529948 KI270467.1 1890 G C, T, A 537 55 397 19 0.033804 0.168296776 chrX 4.9E+07 G A 20 35 292 116 3.61E−06 0.167685515 chr1 1.7E+08 T C 36 12 399 36 0.003711 0.167241379 chr6 3.3E+07 C T 16 36 169 106 0.000562 0.16703464 KI270466.1 651 G A 371 60 474 39 0.012383 0.167007352 chr17 7014384 C T 27 75 396 250 1.07E−09 0.166939444 chr6 3.1E+07 C A 44 167 727 579 4.09E−19 0.166791848 chr6 3.1E+07 G C 51 182 796 622 2.43E−20 0.166155644 chr9 7.3E+07 A C 7 19 134 70 0.001929 0.163837756 chrM 2001 C T 296 472 1170 819 6.00E−20 0.163698097 chr19 1.8E+07 G C 135 79 254 76 0.003889 0.162633718 chr6 3.1E+07 C A 57 200 882 697 1.11E−21 0.162262565 chr11 6.5E+07 A G 590 204 514 89 8.31E−06 0.161825444 KI270467.1 2796 T C, A, G 846 132 246 9 0.000107 0.161444938 chr14 2.7E+07 A G 493 121 451 56 0.000721 0.161370056 chr6 3.1E+07 A G 77 213 831 653 2.41E−18 0.161156667 chr6 3.1E+07 T C 62 188 776 613 7.56E−18 0.160720937 chr21 4.5E+07 T C 174 56 203 34 0.042662 0.160683761 chr15 3.5E+07 C T 437 163 569 111 2.73E−05 0.160496873 chr6 3.1E+07 A G 53 182 795 636 4.16E−19 0.159993888 chr6 3E+07 T G, A 19 29 289 102 1.83E−05 0.159685734 chr6 3.1E+07 G C 42 168 790 632 4.93E−20 0.159519231 KI270467.1 1836 A T, C, G 71 10 568 27 0.041011 0.159159159 chrX 1.5E+08 G A 414 110 421 58 0.001499 0.158953522 KI270466.1 207 G T, A 460 75 440 37 0.011614 0.158531746 chr6 3.1E+07 G A 54 176 771 611 6.03E−18 0.158179508 chr6 3.1E+07 T C 69 205 830 668 1.96E−18 0.158070505 chr6 4.4E+07 G A 5 12 149 51 0.00163 0.158008658 chr6 3.1E+07 A T 54 195 909 716 6.78E−22 0.157975728 chr6 3E+07 C T 71 171 748 529 2.20E−15 0.157594628 chr6 3.1E+07 C T 56 171 711 571 4.89E−16 0.157446487 chr15 3.5E+07 G A 477 155 467 79 0.000166 0.157096552 chr6 3.3E+07 G A 3 10 90 43 0.020259 0.156421181 chrX 1.5E+08 C T 490 112 326 36 0.002656 0.156266561 chr6 3.3E+07 C T 40 77 206 165 0.000611 0.155580192 chr6 3.3E+07 C G 20 46 169 130 0.001282 0.155543531 chr12 1.1E+07 T C 119 66 186 55 0.025556 0.155290611 chr6 3.1E+07 T G 56 193 907 717 8.22E−21 0.153936303 chr17 7312217 T C, G 16 35 106 88 0.026097 0.153405305 chr11 6.5E+07 C G 265 138 444 124 0.000181 0.15295169 chr10 7806641 A G 9 8 204 33 0.006648 0.15286843 chr11 6.6E+07 C T 90 243 1357 890 4.21E−28 0.152277195 chr6 3.1E+07 A C 49 163 745 599 1.00E−16 0.152197915 chr6 4.4E+07 T A 29 47 204 123 0.001276 0.152007069 chr6 3.3E+07 C G 95 167 460 350 1.18E−07 0.151846237 KI270466.1 379 G C, T 692 127 328 26 0.001306 0.151633987 KI270466.1 131 T C 508 79 399 32 0.01685 0.151623509 chr6 3.1E+07 T G 51 163 747 594 2.03E−16 0.151413872 chr11 1.1E+08 G A 646 556 473 207 3.31E−10 0.151401328 chr11 6.5E+07 C T 529 158 453 71 0.000315 0.151259794 chr6 3E+07 A G 16 29 375 122 3.00E−07 0.151132264 chr7 1.4E+08 C T 236 36 818 60 0.007479 0.151091082 KI270466.1 430 A G, T 719 135 318 25 0.000882 0.150403809 chr6 3.1E+07 G A 62 195 894 711 3.32E−19 0.150378232 chr10 4.5E+07 G A 382 118 284 45 0.003649 0.150352807 chr6 2.6E+07 G A 4 5 340 26 3.43E−05 0.149662416 chr6 3.1E+07 G A 54 187 924 726 1.43E−20 0.149604553 chr10 7.2E+07 T A 295 104 629 118 0.000284 0.149204399 chr6 3.1E+07 C G, T 51 168 793 635 3.05E−17 0.148788902 chr6 3.1E+07 G A 88 205 771 611 3.26E−14 0.148780787 KI270467.1 1465 G T, C 670 68 242 9 0.030975 0.14846776 chr6 3.1E+07 G A 55 170 781 625 1.95E−16 0.148047004 chr6 3.1E+07 G A 70 197 877 691 1.31E−17 0.147929212 chr6 3.1E+07 C T 50 167 792 639 3.55E−17 0.147813607 chr6 3.1E+07 A T, C, G 67 177 712 580 6.75E−14 0.147809999 KI270467.1 3778 G A, C 659 77 322 17 0.024534 0.14738543 chr6 3E+07 T C 58 168 947 653 1.68E−19 0.146917059 chrX 1.5E+08 C T 588 153 465 64 0.000619 0.146664566 KI270467.1 2631 T G 896 180 313 23 7.71E−05 0.145591153 chr11 6.5E+07 G C 198 54 339 51 0.036146 0.14557063 chr7 1.3E+08 G A 276 93 556 102 0.001287 0.145192308 chr6 3.1E+07 A G 79 204 877 692 1.79E−16 0.145042588 chr11 6.5E+07 T A 167 48 398 61 0.022263 0.144791751 chr6 3.1E+07 C T 52 160 762 607 1.25E−15 0.14472289 chr6 3.1E+07 T C 108 214 689 550 3.61E−11 0.144596556 chrX 7.4E+07 A C, G, T 707 400 470 137 1.02E−07 0.144199263 chr6 3.3E+07 C G, A 103 172 468 358 4.99E−07 0.144143013 KI270466.1 673 T C, G, A 93 27 780 81 0.000256 0.14347079 chr6 3.1E+07 G C 101 216 630 551 2.15E−10 0.143449794 chr6 3.1E+07 A G 87 212 879 697 9.57E−16 0.143161211 chr6 3.1E+07 C A 101 213 758 604 1.46E−12 0.143131335 chr6 3.3E+07 C G 19 43 190 141 0.001218 0.142786561 chr6 3E+07 A T, C 67 170 916 637 4.11E−17 0.142498056 chr6 3E+07 T C 23 30 359 118 1.78E−05 0.142493279 chr6 3.1E+07 T C 56 168 802 641 6.40E−16 0.142395717 chr11 6.5E+07 G A, C 778 340 501 113 7.24E−07 0.142264152 chr6 3.1E+07 T G 54 178 916 723 8.16E−19 0.141888165 chr6 3.3E+07 C T 19 44 195 147 0.001011 0.141581445 chr6 3.1E+07 G C 52 158 749 609 8.50E−15 0.141078541 chr6 3.1E+07 C T 48 173 922 737 2.07E−19 0.140625354 chr6 3.1E+07 T A 50 158 759 623 3.72E−15 0.14050004 chrX 7.4E+07 G A 7 9 130 38 0.040018 0.140394471 chr6 3E+07 A T 12 27 394 132 1.05E−07 0.140254671 KI270467.1 2782 C T, G 625 87 367 26 0.023395 0.139871182 chr6 3.3E+07 A C 23 49 163 137 0.005936 0.139784946 chrM 1948 C T 335 474 1132 814 2.39E−14 0.139655231 KI270466.1 690 G C 601 81 252 15 0.029549 0.139177902 chr6 3.1E+07 G A 58 167 799 643 4.78E−15 0.138494893 chr6 3.1E+07 G C, T 49 150 761 605 8.60E−15 0.13818167 chr4 2.2E+07 T C 26 7 435 29 0.022296 0.138045312 KI270467.1 3652 T A, C 769 98 274 14 0.011087 0.137703739 chr6 3E+07 T A 63 157 810 595 3.13E−14 0.136611647 chr6 3.1E+07 C T 103 219 857 680 2.18E−13 0.136312338 chr15 3.5E+07 G A 266 103 664 141 0.000445 0.136109642 chr6 3.3E+07 C A 23 45 180 136 0.005035 0.135318292 chr6 3.1E+07 T C 79 189 823 661 1.32E−13 0.134769793 chr6 3.3E+07 T C 25 47 179 136 0.006384 0.134281581 chr14 3.9E+07 A G 207 90 288 73 0.020202 0.133965421 chr6 3.3E+07 C G, T 108 168 458 350 6.04E−06 0.133511603 chr6 3E+07 C T 63 156 873 622 6.95E−15 0.133206447 KI270467.1 3620 A G 747 108 314 21 0.011535 0.133156522 chr3 1.9E+08 C T 312 276 712 355 5.70E−07 0.132713451 chr6 3.3E+07 A G 116 179 497 377 2.50E−06 0.132709167 chr14 1.1E+08 T G, A, C 118 192 50 38 0.013479 0.132401656 chr6 3.3E+07 A C 25 51 198 159 0.003396 0.13074952 chr19 1.8E+07 T A 143 73 237 71 0.043645 0.130628655 chr6 3.3E+07 T G, A 108 172 486 379 3.90E−06 0.130341528 chr6 3.3E+07 G T 102 165 517 396 1.63E−06 0.129335741 chr3 1.9E+08 T C 338 284 679 332 2.65E−06 0.128688912 chr6 3E+07 T G 45 146 975 701 5.00E−18 0.128255434 chr6 3E+07 A T, G 47 149 976 707 7.83E−18 0.128122117 KI270466.1 410 T A 736 136 306 27 0.005508 0.128021855 chr6 3.3E+07 C G 17 42 203 163 0.001591 0.127605322 chr6 3E+07 A G 47 149 998 716 4.15E−18 0.127278259 chr6 3E+07 A G 42 133 887 642 4.96E−16 0.126403 KI270467.1 2322 C A, G 1040 225 199 8 5.63E−06 0.126278634 chr6 3.3E+07 C G, A 96 154 478 371 8.20E−06 0.126085947 chr3 1.9E+08 C T 307 269 720 364 2.06E−06 0.126031586 KI270467.1 1904 G A 60 19 963 84 4.10E−05 0.125814993 chr6 3E+07 A G 52 145 941 669 5.86E−16 0.125766112 chr6 3E+07 A G 20 30 403 144 1.07E−05 0.125132469 chr6 3E+07 G C 60 147 868 628 1.12E−13 0.125022247 chr6 3E+07 A G 49 139 863 639 1.34E−14 0.124935169 KI270467.1 1898 T A 73 20 952 82 0.000212 0.124858919 chr6 3E+07 C G 48 143 950 685 2.09E−16 0.124609122 chrM 1905 C T, G, A 398 507 1065 774 1.05E−10 0.123740798 chr6 3E+07 C T 60 152 941 676 7.13E−15 0.123634819 chr14 6.2E+07 T C 5 3 254 18 0.046604 0.123552124 chr6 3.3E+07 G T 108 162 485 369 1.76E−05 0.122959957 chr6 3.3E+07 A G 19 44 151 144 0.019928 0.122277847 chr6 1.7E+08 T G 3 14 192 88 0.000393 0.121870287 chr6 3E+07 G A 29 35 433 155 3.60E−05 0.121439964 KI270467.1 3208 A G 118 33 1024 114 0.000229 0.1211623 chr6 3E+07 C T 19 29 416 147 9.76E−06 0.121094566 KI270467.1 3839 T G, C, A 779 86 178 6 0.028682 0.120780519 chr6 3.3E+07 T G 111 167 502 387 1.74E−05 0.120367371 chr6 3E+07 A C 53 137 851 629 3.63E−13 0.120222856 chr10 1.3E+08 C T 223 129 337 120 0.010766 0.119858003 chr6 3E+07 G C, T 44 136 986 709 2.39E−16 0.118228299 KI270467.1 3823 G C 65 16 889 70 0.001395 0.11791234 chr6 3E+07 A G 71 156 918 667 1.22E−12 0.117760739 chrM 1792 G A 537 620 929 661 6.70E−09 0.117694013 chr6 3.3E+07 C T, G 115 167 493 378 4.21E−05 0.117277282 chr11 6.5E+07 T G, C, A 1103 540 276 49 2.86E−09 0.116953182 chr6 3E+07 A T, C 51 130 861 622 6.82E−13 0.116951288 chr6 3.3E+07 C T 119 171 419 337 0.000299 0.115424582 chr1 6.7E+07 C T 0 3 303 23 1.01E−05 0.115384615 chr6 3E+07 cl T 67 151 940 682 9.05E−13 0.114738249 chr6 3.3E+07 A, T 19 41 209 166 0.005134 0.1147343 chr2 2.2E+08 C T 394 190 272 79 0.00758 0.114728111 chr6 3E+07 C T, G 64 141 815 612 7.94E−11 0.114440985 chr6 3.3E+07 C T, G 109 163 496 391 5.22E−05 0.114058537 chr6 3.3E+07 G C, T 111 168 500 401 4.94E−05 0.113585439 chr6 1.6E+08 A T 20 15 241 64 0.035557 0.113245065 chr6 3E+07 G T 46 133 968 706 8.31E−15 0.113157159 chr6 3E+07 C T 72 152 911 668 1.56E−11 0.112120686 chr10 3136689 C T 341 106 454 90 0.030965 0.111885509 chr6 3.2E+07 A G 73 119 297 266 0.003454 0.111793612 chr6 3.3E+07 T G, A 111 160 483 376 0.000119 0.111638776 chr6 3E+07 G A 57 134 872 641 9.15E−12 0.111546929 chrX 1.5E+08 G A 590 135 401 56 0.025018 0.111448059 chrM 8019 C T 254 367 1199 916 7.59E−11 0.111237588 chr12 1.2E+08 G A 286 201 490 218 0.001544 0.111156903 chr16 3069430 C T 536 444 343 172 8.73E−05 0.110995376 chr6 3.2E+07 G A 64 107 330 285 0.001549 0.110522635 chr10 3136723 C T 294 91 455 90 0.043123 0.110239066 chr6 3E+07 G A 48 131 971 702 4.61E−14 0.1101579 chr17 2.7E+07 A G 544 139 299 45 0.027538 0.110120429 chr6 3E+07 T C 54 129 821 622 5.95E−11 0.110056686 chr6 3E+07 G A 64 148 988 721 6.74E−13 0.1094742 chr6 3.3E+07 A C 109 159 516 402 7.90E−05 0.10902246 chr6 3E+07 C T 24 27 357 130 0.00111 0.108982396 chr1 2563650 G T, A 451 159 334 74 0.021152 0.10788114 chr17 4.9E+07 G C 266 179 360 157 0.009694 0.107817967 chr6 3.3E+07 T C 28 48 195 158 0.027194 0.107449171 chr6 3.2E+07 T G 64 103 277 246 0.007575 0.107445655 chr6 3.3E+07 G A 160 205 475 366 0.000486 0.107050761 chrM 9777 G A 553 618 894 646 2.75E−07 0.106754044 KI270466.1 650 A C, T 59 18 787 84 0.002514 0.106730636 chr6 3.3E+07 C T 15 35 200 165 0.00938 0.105232558 chr6 3.3E+07 T C 24 44 208 167 0.017764 0.10508253 chr19 8371280 C T 270 125 365 111 0.037004 0.104464167 chrM 3954 C T 674 658 680 435 3.11E−06 0.104228466 chr6 3.3E+07 G A 16 36 203 168 0.01102 0.103411228 chr6 3E+07 C T 56 134 973 715 3.62E−12 0.103410976 chr6 3.3E+07 A G 22 42 210 170 0.016346 0.103285621 chr6 3.3E+07 T G 131 178 467 375 0.000748 0.102817106 chr6 3.3E+07 A G 117 163 464 373 0.000687 0.102727541 chr6 3.3E+07 C A 149 191 455 356 0.001195 0.102488589 chr6 3.3E+07 G A, C 103 148 479 382 0.000439 0.102269338 chr6 3.3E+07 T C 136 180 494 386 0.000575 0.102148186 chr3 1.9E+08 C G 338 229 438 197 0.005125 0.101991675 chr6 3.3E+07 T G, A 86 132 478 387 0.000298 0.101852991 chr6 3E+07 G T 28 119 1144 831 3.80E−18 0.101372373 chr6 3E+07 G A 47 130 1056 776 6.50E−14 0.100876798 chr1 6.7E+07 A T 2 3 317 25 0.003177 0.100873265 chr11 6.5E+07 A G 1001 1057 479 303 2.62E−08 0.100854531 chr20 4.9E+07 T G, A, C 288 141 68 14 0.032722 0.100688655 chrM 7792 C T 594 650 855 623 1.72E−06 0.100666982 chr14 1.1E+08 C T, G 39 91 238 286 0.011087 0.100585087 Gene CHR Name RefSeq_ID Coding Func_type Mutation_type Cosmic chr2 MIR4435-1; . intergenic . haematopoietic_and_lymphoid_tissue; ANAPC1P4 cervix; lung chr6 HLA-C . intronic . lung; large_intestine chr6 HLA-C . intronic . large_intestine chr6 HLA-C . intronic . large_intestine chr6 HLA-C . intronic . lung; large_intestine chr3 MRPS25 . intronic . large_intestine chr17 KRBA2 . UTR5 . salivary_gland; large_intestine; urinary_tract; liver; stomach; haematopoietic_and_lymphoid_tissue chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue chr2 LOC100506474; . intergenic . pancreas LINC00276 chr6 HLA-C . intronic . large_intestine chr2 LOC100506474; . intergenic . pancreas LINC00276 chr6 HLA-C . intronic . large_intestine chr6 HLA-C . intronic . lung; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr8 GPAT4 . intronic . oesophagus KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver chr3 EXOG . intronic . oesophagus KI270467.1 NONE; NONE . intergenic . liver chr2 LOC100506474; . intergenic . pancreas LINC00276 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr2 LOC100506474; . intergenic . pancreas LINC00276 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr17 FLII NM_001256265 c.C3612T exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; large_intestine; thyroid; urinary_tract; lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-F . intronic . thyroid KI270467.1 NONE; NONE . intergenic . liver chr17 SAT2; . intronic . liver; SHBG lung; large_intestine; haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr2 LOC100506474; . intergenic . pancreas LINC00276 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-C . intronic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-F . intronic . haematopoietic_and_lymphoid_tissue; lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr3 PDCD6IP . intronic . oesophagus KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr3 MITF . intronic . oesophagus KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 LOC101929705 . ncRNA_intronic . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 EZR . intronic . large_intestine chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chrX FTX . ncRNA_intronic . large_intestine; lung; eye KI270467.1 NONE; NONE . intergenic . liver chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr1 MTR . intronic . haematopoietic_and_lymphoid_tissue; prostate KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-C . UTR5 . oesophagus; thyroid; biliary_tract chr6 HLA-C . UTR5 . upper_aerodigestive_tract; urinary_tract; haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver chr4 UFSP2 . upstream . skin KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr2 LOC100506474; . intergenic . pancreas LINC00276 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr3 BHLHE40 . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 MALAT1; . ncRNA_exonic . lung TALAM1 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr2 LOC100506474; . intergenic . pancreas LINC00276 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr6 EZR . intronic . large_intestine KI270467.1 NONE; NONE . intergenic . liver chr17 SAT2 NM_001320845 c.G531A exonic synonymous_SNV salivary_gland; large_intestine; urinary_tract; liver; stomach; haematopoietic_and_lymphoid_tissue chr3 BHLHE40-AS1 . ncRNA_intronic . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr9 EHMT1 NM_001354259 c.T351C exonic synonymous_SNV large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr8 CYRIB . intronic . haematopoietic_and_lymphoid_tissue; large_intestine; lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung chr3 MITF . intronic . oesophagus KI270467.1 NONE; NONE . intergenic . liver chr12 RMST . ncRNA_intronic . large_intestine; pancreas; haematopoietic_and_lymphoid_tissue chr1 PRRC2C; . intergenic . liver; MYOCOS breast KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic . liver chr6 PSORS1C3 . ncRNA_intronic . central_nervous_system; urinary_tract; lung chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver KI270467.1 NONE; NONE . intergenic liver chr9 CCDC107 NM_174923 c.A658G exonic nonsynonymous_SNV salivary_gland chr6 HLA-A . intronic . upper_aerodigestive_tract; soft_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr6 PRPF4B NM_003913 c.A247G exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; lung chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI2704671 NONE; NONE . intergenic . liver KI2704671 NONE; NONE . intergenic . liver chr12 RMST . ncRNA_intronic . large_intestine; pancreas; haematopoietic_and_lymphoid_tissue chr11 MALAT1; . ncRNA_exonic . central_nervous_system TALAM1 KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 . UTR3 . liver KI270467.1 NONE; NONE . intergenic . liver chr6 PSORS1C3 . ncRNA_intronic . central_nervous_system; urinary_tract; lung KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A . intronic . liver; eye; upper_aerodigestive_tract; lung; large_intestine; thyroid; NS KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chrX TSIX; . ncRNA_exonic . large_intestine; XIST lung; eye chrX FTX . ncRNA_intronic . large_intestine; lung; eye KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr4 KCNIP4 . intronic . lung chrX TSIX; . neRNA_exonic . large_intestine; XIST lung; eye chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung chr19 CLEC11A NM_002975 c.G882A exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; salivary_gland; lung KI270466.1 NONE; NONE . intergenic . liver chr3 AZI2 . intronic . large_intestine chr6 H4C8; . intergenic . haematopoietic_and_lymphoid_tissue; BTN3A2 lung KI270467.1 NONE; NONE . intergenic . liver chr6 PSORS1C3 . ncRNA_intronic . central_nervous_system; urinary_tract; lung chr6 VEGFA . UTR3 . lung chr6 HLA-A . intronic . haematopoietic_and_lymphoid_tissue chr3 BHLHE40 . UTR3 . lung KI2704671 NONE; NONE . intergenic . liver chr8 GPAT4 . intronic . liver KI270467.1 NONE; NONE . intergenic . liver chr19 ANGPTL4 . intronic . haematopoietic_and_lymphoid_tissue chr6 HLA-A . intronic . thyroid chr4 KCNIP4 . intronic . lung KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr8 ZNF252P-AS1; . intergenic . bone C80rf33 KI270467.1 NONE; NONE . intergenic . liver chr6 RPP21; NM_001199120 c.C469A exonic nonsynonymous_SNV thyroid TRIM39-RPP21 KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A . intronic . thyroid chr4 KCNIP4 . intronic . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chrX FTX . ncRNA_intronic . large_intestine; lung; eye chr6 SRSF3 . UTR3 . lung KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 . UTR3 . liver chr11 NEAT1 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver chr15 LINC02256 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue chr4 KCNIP4 . intronic . lung chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chrX TSIX; . ncRNA_exonic . large_intestine; lung; eye XIST chrX FTX . neRNA_intronic . large_intestine; lung; eye chr5 STING1; . intergenic . pancreas UBE2D2 chr6 TAPBP . UTR3 . central_nervous_system KI270467.1 NONE; NONE . intergenic . liver chrX FTX . ncRNA_intronic . large_intestine; lung; eye KI270467.1 NONE; NONE . intergenic . liver KI2704671 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver chr18 ZNF532; . intergenic . large_intestine; kidney; OACYLP haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver chr4 KCNIP4 . intronic . lung chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr11 NEAT1 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue chr3 GBE1 . UTR5 . oesophagus chrX XIST . ncRNA_exonic . large_intestine; lung; eye chr2 LINC01320 . ncRNA_exonic . thyroid chr8 GPAT4 . intronic . liver KI270467.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver chr19 ANGPTL4 . UTR5 . haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver chr8 NDRG1 . intronic . prostate chr9 CEMIP2 . intronic . liver; large_intestine KI270467.1 NONE; NONE . intergenic . liver chr11 PGGHG . UTR3 . lung KI270466.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chrX ATRX NM_138270 c.G2671C exonic nonsynonymous_SNV large_intestine; lung; eye KI270466.1 NONE; NONE . intergenic . liver chr11 MALAT1; . ncRNA_exonic . central_nervous_system TALAM1 chrX FTX . ncRNA_intronic . large_intestine; lung; eye chr11 PGGHG . UTR3 . lung chr9 DPP7 NM_013379 c.T162C exonic synonymous_SNV thyroid; large_intestine; skin; prostate KI270467.1 NONE; NONE . intergenic . liver chr8 NDRG1 . intronic . prostate chr4 KCNIP4 . intronic . lung chr6 HLA-A . intronic . thyroid KI270466.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver chr11 MALAT1; . ncRNA_exonic . lung TALAM1 KI270466.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr4 UFSP2 . upstream . skin KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr8 NDRG1 . intronic . lung; haematopoietic_and_lymphoid_tissue chr6 HLA-C NM_001243042 c.A985G exonic nonsynonymous_SNV thyroid; large_intestine; haematopoietic_and_lymphoid_tissue chrX XIST . ncRNA_exonic . large_intestine; lung; eye chr6 RPP21; NM_001199120 c.T468G exonic synonymous_SNV upper_aerodigestive_tract TRIM39-RPP21 chr11 PGGHG . UTR3 . lung chr6 HLA-C NM_001243042 c.G201A exonic synonymous_SNV oesophagus; lung; haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver chr8 NDRG1 . intronic . lung; haematopoietic_and_lymphoid_tissue chr2 UGT1A6 NM_001072 c.T19G exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; skin; large_intestine; thyroid; lung chr11 PGGHG . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver KI270467.1 NONE; NONE . intergenic . liver chr7 HILPDA . UTR3 . lung KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 . UTR5 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr14 HNRNPC . intronic . prostate chr1 PAPPA2 . UTR5 . pancreas KI270467.1 NONE; NONE . intergenic . liver chr8 GPAT4 . intronic . liver chr6 HLA-C NM_001243042 c.A872T exonic nonsynonymous_SNV upper_aerodigestive_tract; large_intestine; oesophagus chrX FTX . ncRNA_intronic . large_intestine; lung; eye chr6 HMGN3 . intronic . lung KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . neRNA_exonic . haematopoietic_and_lymphoid_tissue chr16 GALNS . UTR3 . thyroid chr6 HLA-C NM_001243042 c.C989T exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; thyroid; large_intestine; upper_aerodigestive_tract chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue chr11 NDUFV1 . intronic . haematopoietic_and_lymphoid_tissue chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 LOC101927730 . ncRNA_intronic . biliary_tract chr6 RPP21; . UTR3 . thyroid TRIM39-RPP21 chr6 LOC101927730 . ncRNA_intronic . biliary_tract chr6 HLA-A . intronic . large_intestine; liver chrX RRAGB . UTR5 . pancreas chr11 NDUFV1 . intronic . haematopoietic_and_lymphoid_tissue chr4 KCNIP4 . intronic . lung chr2 LINC01320 . neRNA_exonic . thyroid chr6 HLA-C NM_001243042 c.G47C exonic nonsynonymous_SNV thyroid; oesophagus; biliarytract chr6 HLA-C . UTR5 . oesophagus; thyroid; biliary_tract chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-C NM_001243042 c.C28G exonic nonsynonymous_SNV oesophagus; thyroid chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A . intronic . large_intestine; liver KI270467.1 NONE; NONE . intergenic . liver chr8 NDRG1 . intronic . prostate chr6 HLA-C NM_001243042 c.T512A exonic stopgain upper_aerodigestive_tract; biliary_tract; thyroid chr19 GDF15 NM_004864 c.G420T exonic synonymous_SNV stomach; large_intestine chr6 HLA-C NM_001243042 c.T972A exonic synonymous_SNV bone; kidney KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A . intronic . soft_tissue; liver; large_intestine chr6 HLA-C NM_001243042 c.A991G exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; largeintestine; thyroid KI270467.1 NONE; NONE . intergenic . liver chrX RBM3 . UTR3 . lung chr1 PRRC2C; . intergenic . liver; breast MYOCOS chr6 HLA-DMA NM_006120 c.G496A exonic nonsynonymous_SNV central_nervous_system KI270466.1 NONE; NONE . intergenic . liver chr17 RNASEK-C17orf49 . ncRNA_exonic . thyroid; haematopoietic_and_lymphoid_tissue chr6 HLA-C NM_001243042 c.G97T exonic nonsynonymous_SNV large_intestine; oesophagus; autonomic_ganglia chr6 HLA-C NM_001243042 c.C984G exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; large_intestine; thyroid chr9 ANXA1 NM_000700 c.A931C exonic nonsynonymous_SNV liver; large_intestine chrM NONE; MIR12136 . intergenic . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr19 GDF15 NM_004864 c.G25C exonic nonsynonymous_SNV lung; large_intestine; stomach; skin chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr11 NEAT1 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue KI270467.1 NONE; NONE . intergenic . liver chr14 NOVA1 . UTR5 . large_intestine chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr6 HLA-C NM_001243042 c.A925G exonic nonsynonymous_SNV lung; pancreas chr21 UBE2G2 . UTR3 . soft_tissue chr15 GOLGA8B . UTR5 . haematopoietic_and_lymphoid_tissue chr6 HLA-C NM_001243042 c.T956C exonic nonsynonymous_SNV pancreas; haematopoietic_and_lymphoid_tissue; kidney; thyroid; large_intestine chr6 HLA-A . intronic . large_intestine chr6 HLA-C NM_001243042 c.C829G exonic nonsynonymous_SNV upper_aerodigestive_tract; large_intestine; oesophagus KI270467.1 NONE; NONE . intergenic . liver chrX SLC6A8 . UTR3 . lung KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-C NM_001243042 c.C987T exonic synonymous_SNV upper_aerodigestive_tract; thyroid; large_intestine; haematopoietic_and_lymphoid_tissue chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr6 MRPL14 NM_001318771 c.C198T exonic synonymous_SNV lung chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr6 HLA-A NM_001242758 c.C41T exonic nonsynonymous_SNV thyroid chr6 HLA-C NM_001243042 c.G22A exonic nonsynonymous_SNV oesophagus; thyroid; biliary_tract chr15 GOLGA8B . UTR5 . haematopoietic_and_lymphoid_tissue chr6 HLA-DRB1 . intronic . haematopoietic_and_lymphoid_tissue; liver; large_intestine chrX PNCK NM_001039582 c.G817A exonic nonsynonymous_SNV endometrium chr6 HLA-DQA1 NM_002122 c.C60T exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DQA1 NM_002122 c.C705G exonic synonymous_SNV upper_aerodigestive_tract; kidney; large_intestine; oesophagus; thyroid; haematopoietic_and_lymphoid_tissue chr12 PRH1-PRR4 . ncRNA_exonic . haematopoietic_and_lymphoid_tissue; biliary_tract chr6 HLA-C . UTR3 . large_intestine chr17 EIF5A . UTR3 . thyroid; haematopoietic_and_lymphoid_tissue chr11 NEAT1 . neRNA_exonic . thyroid; large_intestine chr10 ATP5F1C . intronic . haematopoietic_and_lymphoid_tissue; lung chr11 MALAT1; . ncRNA_exonic . lung TALAM1 chr6 HLA-C NM_001243042 c.T142G exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; breast; thyroid; oesophagus; large_intestine; liver; biliary_tract; upper_aerodigestive_tract chr6 HSP90AB1 . UTR5 . lung chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine KI270466.1 NONE; NONE . intergenic . liver KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-C NM_001243042 c.A341C exonic nonsynonymous_SNV upper_aerodigestive_tract; thyroid; lung chr11 CRYAB NM_001368246 c.C286T exonic nonsynonymous_SNV biliary_tract; liver chr11 NEAT1 . neRNA_exonic . thyroid; large_intestine chr6 HLA-A . intronic . biliary_tract chr7 CYREN; . intergenic . large_intestine; WDR91 haematopoietic_and_lymphoid_tissue KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-C . UTR3 . large_intestine chr10 DEPP1 NM_007021 c.C611T exonic nonsynonymous_SNV liver chr6 H4C8; . intergenic . haematopoietic_and_lymphoid_tissue; BTN3A2 lung chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr10 DDIT4 . UTR3 . lung chr6 HLA-C NM_001243042 c.G735A exonic synonymous_SNV thyroid; large_intestine; lung; upper_aerodigestive_tract; eye; haematopoietic_and_lymphoid_tissue; NS chr6 HLA-C NM_001243042 c.C747T exonic synonymous_SNV large_intestine; thyroid; upper_aerodigestive_tract KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-C NM_001243042 c.C650T exonic nonsynonymous_SNV thyroid chr6 HLA-C . UTR3 . large_intestine chr6 HLA-C NM_001243042 c.G744A exonic synonymous_SNV large_intestine; oesophagus; thyroid; upper_aerodigestive_tract chr6 HLA-C NM_001243042 c.T539C exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; skin; thyroid; upper_aerodigestive_tract; central_nervous_system; biliary_tract KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A NM_001242758 c.T1029C exonic synonymous_SNV soft_tissue; central_nervous_system; upper_aerodigestive_tract chrX PNCK . UTR3 . biliary_tract; thyroid KI270467.1 NONE; NONE . intergenic . liver chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr7 HILPDA . UTR3 . lung chr6 HLA-C . UTR3 . large_intestine chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr6 HLA-C NM_001243042 c.G289A exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; upper_aerodigestive_tract; central_nervous_system chr6 HLA-C NM_001243042 c.A873G exonic synonymous_SNV large_intestine; oesophagus; central_nervous_system chrX TSIX; . ncRNA_exonic . large_intestine; lung; eye XIST chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-B NM_005514 c.C44A exonic nonsynonymous_SNV lung; haematopoietic_and_lymphoid_tissue; soft_tissue chr6 HLA-C . UTR3 . large_intestine chr6 HLA-C NM_001243042 c.G993T exonic nonsynonymous_SNV upper_aerodigestive_tract; thyroid chr6 HLA-DQA1 NM_002122 c.C714G exonic nonsynonymous_SNV upper_aerodigestive_tract; kidney; large_intestine; oesophagus; thyroid; haematopoietic_and_lymphoid_tissue chr6 HLA-A NM_001242758 c.A1033C exonic nonsynonymous_SNV soft_tissue; central_nervous_system; liver; upper_aerodigestive_tract chr6 HLA-A . intronic . biliary_tract chr6 HLA-C NM_001243042 c.A853G exonic nonsynonymous_SNV oesophagus; upper_aerodigestive_tract chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr6 HLA-C . UTR3 . large_intestine chr6 HLA-DQA1 NM_002122 c.C591T exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-C NM_001243042 c.C486G exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; thyroid; liver; central_nervous_system; biliary_tract; upper_aerodigestive_tract chr6 HLA-C . UTR3 . central_nervous_system; urinary_tract; lung chr6 HLA-C NM_001243042 c.A361T exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; skin; thyroid; large_intestine; lung chrX FTX . ncRNA_intronic . large_intestine; lung; eye chr6 HLA-A . intronic . large_intestine KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 NM_002122 c.A438C exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; upper_aerodigestive_tract; thyroid; large_intestine chrM NONE; MIR12136 . intergenic . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-C NM_001243042 c.C648T exonic synonymous_SNV thyroid chr6 HLA-C NM_001243042 c.C652A exonic nonsynonymous_SNV thyroid chr4 KCNIP4 . intronic . lung KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A NM_001242758 c.T97A exonic nonsynonymous_SNV thyroid chr6 HLA-C . UTR3 . large_intestine chr15 GOLGA8B . UTR5 . haematopoietic_and_lymphoid_tissue chr6 HLA-DQA1 NM_002122 c.C630A exonic synonymous_SNV upper_aerodigestive_tract; kidney; large_intestine; oesophagus; thyroid; haematopoietic_and_lymphoid_tissue chr6 HLA-C NM_001243042 c.A1087G exonic nonsynonymous_SNV thyroid chr6 HLA-DQA1 NM_002122 c.T708C exonic synonymous_SNV upper_aerodigestive_tract; kidney; large_intestine; oesophagus; thyroid; haematopoietic_and_lymphoid_tissue chr14 PNN . UTR3 . breast; stomach; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A NM_001242758 c.C448T exonic synonymous_SNV soft_tissue; urinary_tract; lung KI270467.1 NONE; NONE . intergenic . liver chr3 LINC02041; . intergenic . liver SST chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr14 ADAM6; . intergenic . ovary LINC00226 chr6 HLA-DQA1 NM_002122 c.A223C exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; thyroid chr19 GDF15 NM_004864 c.T142A exonic nonsynonymous_SNV lung; stomach; haematopoietic_and_lymphoid_tissue; soft_tissue; skin chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr3 LINC02041; . intergenic . liver SST chr6 HLA-A NM_001242758 c.T992G exonic nonsynonymous_SNV liver; eye; upper_aerodigestive_tract; lung; large_intestine; thyroid; NS chr6 HLA-A NM_001242758 c.A964G exonic nonsynonymous_SNV large_intestine KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 NM_002122 c.C169G exonic nonsynonymous_SNV lung; thyroid; biliary_tract; stomach; breast chr6 HLA-A NM_001242758 c.A967G exonic nonsynonymous_SNV soft_tissue; thyroid; upper_aerodigestive_tract; central_nervous_system chr6 HLA-A NM_001242758 c.A363G exonic nonsynonymous_SNV thyroid; soft_tissue KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr3 LINC02041; . intergenic . liver SST KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A NM_001242758 c.A652G exonic nonsynonymous_SNV upper_aerodigestive_tract; thyroid; large_intestine; skin chr6 HLA-A . intronic . haematopoietic_and_lymphoid_tissue; large_intestine chr6 HLA-A NM_001242758 c.G259C exonic nonsynonymous_SNV thyroid chr6 HLA-A NM_001242758 c.A524G exonic nonsynonymous_SNV upper_aerodigestive_tract; stomach KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A NM_001242758 c.C649G exonic nonsynonymous_SNV lung; large_intestine; thyroid; upper_aerodigestive_tract chrM NONE; MIR12136 . intergenic . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr6 HLA-A NM_001242758 c.C651T exonic synonymous_SNV lung; thyroid; large_intestine; skin chr14 HIF1A-AS3 . ncRNA_intronic . thyroid; haematopoietic_and_lymphoid_tissue chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DQB1-AS1 . neRNA_exonic . liver chr6 MPC1 . UTR3 . liver; breast chr6 HLA-A . intronic . large_intestine KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A . intronic . haematopoietic_and_lymphoid_tissue; large_intestine KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A NM_001242758 c.A502C exonic nonsynonymous_SNV upper_aerodigestive_tract; lung chr10 BNIP3 . UTR3 . stomach; large_intestine chr6 HLA-A NM_001242758 c.G829T exonic stopgain breast; soft_tissue; skin; lung; large_intestine; cervix; central_nervous_system; upper_aerodigestive_tract KI270467.1 NONE; NONE . intergenic . liver chr6 HLA-A NM_001242758 c.A633G exonic synonymous_SNV thyroid; large_intestine; skin chrM NONE; MIR12136 . intergenic . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr11 NEAT1 . ncRNA_exonic . thyroid; large_intestine chr6 HLA-A NM_001242758 c.A257C exonic nonsynonymous_SNV thyroid chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr1 SGIP1 . intronic . stomach chr6 HLA-A NM_001242758 c.C642T exonic synonymous_SNV thyroid; skin chr6 HLA-DQA1 NM_002122 c.A143T exonic nonsynonymous_SNV thyroid; urinary_tract; lung; pancreas; haematopoietic_and_lymphoid_tissue chr2 SERPINE2 . UTR3 . lung; large_intestine chr6 HLA-A NM_001242758 c.C180G exonic nonsynonymous_SNV thyroid chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 EZR . UTR3 . large_intestine chr6 HLA-A NM_001242758 c.G808T exonic nonsynonymous_SNV soft_tissue; NS; lung; upper_aerodigestive_tract; eye; central_nervous_system chr6 HLA-A NM_001242758 c.C762T exonic synonymous_SNV haematopoietic_and_lymphoid_tissue; soft_tissue; skin; thyroid chr10 PFKP . UTR3 . lung chr6 HLA-DRA . UTR3 . upper_aerodigestive_tract chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A NM_001242758 c.G219A exonic synonymous_SNV thyroid chrX PNCK . UTR3 . biliary_tract; thyroid chrM MIR12136 . upstream . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr12 UBC NM_021009 c.C1257T exonic synonymous_SNV breast; thyroid; lung; upper_aerodigestive_tract; cervix chr16 IL32 . UTR3 . lung; liver chr6 HLA-DRA . UTR3 . upper_aerodigestive_tract chr10 PFKP . UTR3 . large_intestine chr6 HLA-A NM_001242758 c.G691A exonic nonsynonymous_SNV thyroid; large_intestine; upper_aerodigestive_tract; central_nervous_system; eye; soft_tissue; haematopoietic_and_lymphoid_tissue; skin chr17 WSB1 . UTR3 . haematopoietic_and_lymphoid_tissue; large_intestine; thyroid; urinary_tract; lung chr6 HLA-A NM_001242758 c.T98C exonic nonsynonymous_SNV thyroid chr6 HLA-A NM_001242758 c.G945A exonic synonymous_SNV eye; large_intestine; NS; soft_tissue; haematopoietic_and_lymphoid_tissue chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A . intronic . large_intestine chr1 TNFRSF14 . UTR3 . large_intestine; thyroid; pancreas chr17 ATP5MC1 NM_001002027 c.G351C exonic synonymous_SNV stomach chr6 HLA-DQA1 NM_002122 c.T102C exonic synonymous_SNV kidney; thyroid; pancreas chr6 HLA-DRA NM_019111 c.T724G exonic nonsynonymous_SNV upper_aerodigestive_tract chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chrM MIR12136; . intergenic . large_intestine; breast; NONE haematopoietic_and_lymphoid_tissue; stomach KI270466.1 NONE; NONE . intergenic . liver chr6 HLA-DQA1 NM_002122 c.C177T exonic synonymous_SNV upper_aerodigestive_tract; thyroid chr6 HLA-DQA1 NM_002122 c.T229C exonic nonsynonymous_SNV thyroid chr19 ANGPTL4 NM_001039667 c.C683T exonic nonsynonymous_SNV soft_tissue; large_intestine; salivary_gland; urinary_tract chrM NONE; MIR12136 . intergenic . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr6 HLA-DQA1 NM_002122 c.G224A exonic nonsynonymous_SNV thyroid; urinary_tract chr6 HLA-A NM_001242758 cC987T exonic synonymous_SNV thyroid; skin; soft_tissue chr6 HLA-DQA1 NM_002122 c.A227G exonic nonsynonymous_SNV thyroid; haematopoietic_and_lymphoid_tissue chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 NM_002124 c.C736G exonic nonsynonymous_SNV haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr3 LINC02041; . intergenic . liver SST chr6 HLA-DRB1 . UTR3 . haematopoietic_and_lymphoid_tissue; liver; large_intestine chr6 HLA-A . UTR3 . lung; soft_tissue chr6 HLA-A NM_001242758 c.G1072A exonic nonsynonymous_SNV biliary_tract; urinary_tract; soft_tissue; haematopoietic_and_lymphoid_tissue chr1 SGIP1 . intronic . stomach chr11 MALAT1; . ncRNA_exonic . lung TALAM1 chr20 ZFAS1 . neRNA_intronic . skin chrM MIR12136 . upstream . large_intestine; breast; haematopoietic_and_lymphoid_tissue; stomach chr14 ADAM6; . intergenic . haematopoietic_and_lymphoid_tissue LINC00226

TABLE 15 Expanded mutations in myeloid cells prevalence_ myeloid FDR vs REF_myeloid REF_other ALT_myeloid ALT_other adj others cell cell cell cell P- diff_ALT_ Gene CHR POS REF ALT numbers numbers numbers numbers values cellular Name RefSeq_ID Coding Func_type Mutation_type Cosmic chr6 32444789 T A 30 447 102 212 7.68E−19 0.451027728 HLA- — UTR3 — upper_aerodigestive_ DRA tract chrX 23783269 G T 56 822 159 397 1.38E−26 0.413858099 SAT1 — UTR5 — haematopoietic_and_ lymphoid_tissue; lung chr6 32578838 G A 37 647 124 362 1.63E−19 0.411415275 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32578907 A G 52 753 109 288 2.67E−20 0.400361573 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32444815 G A 31 416 106 248 5.50E−15 0.400228652 HLA- — UTR3 — upper_aerodigestive_ DRA tract chr6 32445032 T A 45 438 82 151 1.26E−14 0.389302568 HLA- — UTR3 — upper_aerodigestive_ DRA tract chr6 32579023 A G 53 734 100 267 1.57E−18 0.386861505 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chrX 23783194 C T 69 920 147 387 2.24E−24 0.384457621 SAT1 — UTR5 — haematopoietic_and_ lymphoid_tissue; lung chr6 32584298 A G 48 596 98 243 3.47E−16 0.381602364 HLA- NM_002124 c.T181C exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32578889 G A 54 745 106 299 8.17E−18 0.376101533 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32584367 A C 30 479 110 334 1.10E−13 0.374890177 HLA- NM_002124 c.T112G exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32578921 G A 54 732 107 310 8.41E−17 0.367091475 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32578920 G A 51 702 110 329 4.08E−16 0.364122151 HLA- — UTR3 — haematopoietic_and_ DRB1 _lymphoid_tissue; liver;large_intestine chr6 32584162 G T, 54 649 94 242 1.52E−15 0.363530197 HLA- NM_002124 c.C317G exonic non- haematopoietic_and_ C DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584315 A T, 32 494 112 358 7.82E−13 0.357589984 HLA- NM_002124 c.T164C exonic non- haematopoietic_and_ G DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584366 C T 36 511 106 330 1.47E−12 0.354088861 HLA- NM_002124 c.G113A exonic stopgain haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32584193 T G 30 494 120 404 7.82E−13 0.350111359 HLA- NM_002124 c.A286C exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32578900 T A 60 744 102 293 1.52E−15 0.347083824 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32579069 C T 49 652 106 339 2.17E−13 0.341792259 HLA- — UTR3 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 32584370 G A 34 465 105 352 3.08E−10 0.32455113 HLA- NM_002124 c.C109T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32581704 C T 71 726 90 243 7.82E−13 0.308232217 HLA- NM_002124 c.G505A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32589702 G A 68 675 77 196 5.00E−12 0.30600578 HLA- NM_002124 c.C41T exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32579025 C G, 61 635 81 231 1.12E−10 0.303678886 HLA- — UTR3 — haematopoietic_and_ A, DRB1 lymphoid_tissue; T liver;large_intestine chr6 32584181 C T 46 545 105 351 6.65E−10 0.303623167 HLA- NM_002124 c.G298A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32589729 T C 67 646 79 211 1.10E−10 0.294888189 HLA- NM_002124 c.A14G exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr7 23274960 A T 16 217 36 145 0.002973 0.291755206 GPNMB — UTR3 — lung;haematopoietic and_lymphoid_ tissue chr6 32581675 C G, 79 772 81 211 1.62E−12 0.291600966 HLA- NM_002124 c.G534A exonic synonymous_ haematopoietic_and_ T DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32584180 G T, 75 676 67 149 5.10E−12 0.291224925 HLA- NM_002124 c.C299G exonic non- haematopoietic_and_ C DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584221 G A 68 663 81 231 4.16E−10 0.285234899 HLA- NM_002124 c.C258T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32581720 G A 79 738 81 222 1.12E−10 0.275 HLA- NM_002124 c.C489T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32581771 G A 77 714 82 228 2.91E−10 0.273685054 HLA- NM_002124 c.C438T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32581786 G A, 80 723 78 206 1.61E−10 0.271927076 HLA- NM_002124 c.C423G exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584354 C G, 27 228 91 228 1.20E−05 0.271186441 HLA- NM_002124 c.G125A exonic\ non- haematopoietic_and_ T DRB1; x3bsplicing synonymous_ lymphoid_tissue; HLA- SNV liver;large_intestine DRB1 chr6 32584283 A T 67 628 80 238 1.33E−08 0.269390897 HLA- NM_002124 c.T196A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32581763 C T, 76 704 84 242 9.77E−10 0.269186047 HLA- NM_002124 c.G446T exonic non- haematopoietic_and_ A DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 29945597 C G 83 1426 99 546 1.47E−11 0.267079776 HLA-A — UTR3 — upper_aerodigestive tract;haematopoietic_ and_lymphoid_ tissue chr6 32517546 T A 40 318 58 156 7.12E−05 0.262722811 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 32581719 C T 62 620 97 335 7.93E−08 0.259277553 HLA- NM_002124 c.G490A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 29945653 C T 86 1456 99 575 2.14E−10 0.252023368 HLA-A — UTR3 — upper_aerodigestive_ tract; lung chr2 60881694 G A 17 120 52 122 0.00676 0.249490957 REL — UTR5 — lung chr6 32589752 C G 73 649 71 209 1.05E−07 0.249465812 HLA- — UTR5 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine chr6 29943309 T C 64 1086 80 481 1.72E−07 0.248599589 HLA-A NM_001242758 c.T385C exonic non- thyroid;urinary_ synonymous_ tract SNV chr6 31533435 A T 26 420 24 127 0.004195 0.247824497 ATP6V1G2- — ncRNA — upper_aerodigestive DDX39B intronic tract; thyroid;skin;h aematopoietic_and lymphoid_tissue chr6 32584151 G A 83 723 67 185 1.73E−08 0.242922173 HLA- NM_002124 c.C328T exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32517630 A T 47 327 54 135 0.000187 0.242445673 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 32584249 C T 75 661 73 222 2.47E−07 0.241827615 HLA- NM_002124 c.G230A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chrX 23783776 C T 61 294 127 226 1.62E−06 0.24091653 SAT1 — intronic — lung chr6 31269077 T C 67 1134 88 566 5.93E−07 0.234800759 HLA-C — UTR3 — upper_aerodigestive_ tract chr6 29943494 G C 79 1204 68 361 8.81E−08 0.231914107 HLA-A NM_001242758 c.G570C exonic non- thyroid;large_intestine; synonymous_ kidney;biliary_ SNV tract;haematopoietic and_lymphoid_ tissue;skin chr6 32517567 G A 42 310 57 163 0.000836 0.231148696 HLA- — UTR3 — upper_aerodigestive_ DRB5 tract chr6 29943462 T C 71 1118 76 453 1.13E−06 0.228655434 HLA-A NM_001242758 c.T538C exonic synony oesophagus; large_ mous intestine; SNV haematopoietic_and_ lymphoid_tissue chr6 29943495 T G, 72 1125 71 416 1.32E−06 0.226548922 HLA-A NM_001242758 c.T571C exonic non- upper_aerodigestive_ A, synonymous_ tract C SNV chr6 29942954 G A 70 1126 71 432 1.94E−06 0.226267537 HLA-A NM_001242758 c.G271A exonic non- thyroid synonymous_ SNV chr6 29943445 C T 69 1117 74 461 2.62E−06 0.225340566 HLA-A NM_001242758 c.C521T exonic non- upper_aerodigestive_ synonymous_ tract; skin SNV chr6 29942965 G C 70 1115 70 436 5.98E−06 0.218891038 HLA-A NM_001242758 c.G282C exonic non- thyroid synonymous_ SNV chr6 32517639 G C 49 330 51 137 0.001259 0.216638116 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 32517455 T G 46 305 50 136 0.002738 0.212443311 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr19 48966392 C T 85 1188 107 627 7.77E−07 0.211837121 FTL NM_000146 c.C361T exonic non- lung;large_intestine; synonymous_ thyroid;haematopoietic_ SNV and_lymphoid_ tissue chr6 32517551 G T, 53 342 48 123 0.001259 0.210731396 HLA- — UTR3 — upper_aerodigestive A DRB5 tract chr6 32584271 C T 87 698 60 175 3.37E−06 0.207705075 HLA- NM_002124 c.G208A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32517566 G A 41 285 60 182 0.004683 0.204337779 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 32517511 G A 46 316 57 171 0.003813 0.202268695 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 29943483 A C 72 1094 74 482 4.71E−05 0.201011752 HLA-A NM_001242758 c.A559C exonic non- thyroid;upper_ synonymous_ aerodigestive_tract SNV chr6 32584266 G A 92 714 56 160 7.50E−06 0.195312017 HLA- NM_002124 c.C213T exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584262 C G 92 703 55 158 1.56E−05 0.190642111 HLA- NM_002124 c.G217C exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32580812 C T 57 558 90 407 0.000684 0.19048324 HLA- NM_002124 c.G697A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 29942886 G A 77 1158 61 397 0.000134 0.186723519 HLA-A NM_001242758 c.G203A exonic non- thyroid synonymous_ SNV chr6 32578849 C G, 5 109 124 377 0.000105 0.185520145 HLA- — UTR3 — haematopoietic_and_ A, DRB1 lymphoid_tissue; T liver;large_intestine chr6 32584273 A T 93 701 53 154 3.83E−05 0.18289674 HLA- NM_002124 c.T206A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32517624 G A 49 315 53 160 0.011719 0.182765738 HLA- — UTR3 — upper_aerodigestive DRB5 tract chr6 29943413 G A 68 1015 78 552 0.000599 0.1819811 HLA-A NM_001242758 c.G489A exonic synonymous_ thyroid SNV chr6 31356374 C T 93 1339 84 554 3.88E−05 0.181919113 HLA-B NM_005514 c.G412A exonic non- soft tissue;urinary_ synonymous_ tract;thyroid; biliary_ SNV tract chr6 29943469 C T 76 1091 72 487 0.000473 0.177867982 HLA-A NM_001242758 c.C545T exonic non- breast;upper_ synonymous_ aerodigestive_tract SNV chr6 31356248 G C 97 1374 77 500 5.38E−05 0.175719771 HLA-B NM_005514 c.C538G exonic non- upper_aerodigestive_ synonymous_ tract SNV chr6 31356246 C G 98 1371 80 519 5.59E−05 0.174835028 HLA-B NM_005514 c.G540C exonic synonymous_ upper_aerodigestive_ SNV tract;haematopoietic_ and_lymphoid_ tissue chr6 32584319 G A 85 654 56 187 0.000447 0.17480878 HLA- NM_002124 c.C160T exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large intestine chr6 32584310 C T 84 650 61 212 0.000585 0.17474998 HLA- NM_002124 c.G169A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr19 18177259 A G 34 139 76 150 0.034642 0.171877949 IFI30 NM_006332 c.A603G exonic synonymous_ skin;stomach;lung SNV chr2 89040248 A G 13 325 82 728 0.010396 0.17179987 MIR4436A; intergenic haematopoietic_and_ LOC107985911 _lymphoid_tissue chr6 32584301 A T 95 711 51 154 0.000124 0.171280386 HLA- NM_002124 c.T178A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 29943463 T G 69 996 80 576 0.001527 0.170500538 HLA-A NM_001242758 c.T539G exonic non- soft_tissue;pancreas; synonymous_ large_intestine SNV chr5 1.5E+08 C T 76 931 117 720 0.000338 0.170118283 CD74 NM_001025158 c.G29A exonic non- kidney synonymous_ SNV chr6 31356934 A C 94 1313 77 513 0.000189 0.169350448 HLA-B NM_005514 c.T97G exonic non- upper_aerodigestive_ synonymous_ tract; central_ SNV nervous_system chr6 31271672 C G 68 1017 60 437 0.002875 0.168199794 HLA-C NM_001243042 c.G270C exonic non- kidney;lung synonymous_ SNV chr6 32584290 C T 94 697 54 171 0.000309 0.167860257 HLA- NM_002124 c.G189A exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32584323 C T 94 699 51 162 0.000442 0.163570828 HLA- NM_002124 c.G156A exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr6 32584316 A T 95 694 50 161 0.000921 0.156523493 HLA- NM_002124 c.T163A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32584309 T A 97 717 46 147 0.000953 0.151539433 HLA- NM_002124 c.A170T exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chrX 1.54E+08 T G 5 167 77 641 0.029694 0.145707559 RPL10 — UTR5 — lung chr5 1.5E+08 G A 93 1025 92 557 0.003152 0.14521133 CD74 NM_001364083 c.C404T exonic non- kidney synonymous_ SNV chr6 32589710 G A 98 706 48 163 0.003304 0.141195202 HLA- NM_002124 c.C33T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large intestine chr6 32584279 A G 97 696 45 152 0.004195 0.137656125 HLA- NM_002124 c.T200A exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr5 1.5E+08 G A, 83 922 107 692 0.008898 0.134409444 CD74 NM_001025158 c.C463A exonic non- haematopoietic_and_ T synonymous_ lymphoid_tissue; SNV breast; lung chr5 1.5E+08 G A 102 1116 100 632 0.005066 0.133493441 CD74 — UTR3 — haematopoietic_and_ _lymphoid_tissue; breast; lung chr6 32584340 A G 96 678 47 167 0.01018 0.131038193 HLA- NM_002124 c.T139C exonic non- haematopoietic_and_ DRB1 synonymous_ lymphoid_tissue; SNV liver;large_intestine chr6 32589688 G A 99 703 47 167 0.008826 0.129963785 HLA- NM_002124 c.C55T exonic synonymous_ haematopoietic_and_ DRB1 SNV lymphoid_tissue; liver;large_intestine chr19 48965830 T C, 34 541 156 1224 0.006011 0.127568212 FTL NM_000146 c.T163G exonic non- lung;large_intestine; G synonymous_ thyroid; SNV haematopoietic_and_ lymphoidtissue chrM 3021 C T 82 1148 131 1163 0.028825 0.111778126 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chr6 32589759 G A 101 682 41 149 0.040776 0.109430349 HLA- — UTR5 — haematopoietic_and_ DRB1 lymphoid_tissue; liver;large_intestine

TABLE 16 Expanded mutations in plasma B cell ALT_ REF_ REF_ other plasma other cell ALT_plasma cell FDR adj diff_ALT_ Gene CHR POS REF ALT B cell numbers B cell numbers P-values cellular Name RefSeq_ID Coding Func_type Mutation_type Cosmic chr14 1.06E+08 C G, 16 397 235 1687 4.41E−05 0.12675402 MIR8071- — intergenic — haematopoietic_ A 1; and_lymphoid MIR8071-1 tissue chr14 1.06E+08 T C 25 249 171 725 0.002818 0.128095797 MIR8071- — intergenic — haematopoietic_ 1; and_lymphoid ELK2AP tissue chr14 1.06E+08 C G 16 212 181 782 0.000653 0.132061404 MIR8071- — intergenic — skin 1; ELK2AP chr14 1.06E+08 T C 26 217 158 635 0.015042 0.113390488 MIR8071- — intergenic — skin 1; ELK2AP chr14 1.06E+08 T C 16 204 163 629 0.00023 0.155512484 MIR8071- — intergenic — thyroid 1; ELK2AP chr14 1.06E+08 C G 45 507 180 807 7.52E−06 0.185844749 MIR8071- — intergenic — large_intestine 1; ELK2AP chr14 1.06E+08 C G 49 528 186 795 2.90E−06 0.190582332 ELK2AP; — intergenic — large_intestine MIR4539 chr14 1.06E+08 T C, 14 246 224 1140 0.000201 0.118665648 ELK2AP; — intergenic — large_intestine A MIR4539 chr14 1.06E+08 G A 54 1184 193 997 1.08E−18 0.324246761 ELK2AP; — intergenic — large_intestine MIR4539 chr14 1.06E+08 G A 73 608 76 363 0.02053 0.136225713 ADAM6; — intergenic — haematopoietic_ LINC00226 and_lymphoid tissue chr14 1.06E+08 G T, 58 432 37 140 0.036763 0.144718439 ADAM6; — intergenic — haematopoietic_ C LINC00226 and_lymphoid tissue chr14 1.06E+08 C T, 102 717 33 117 0.026166 0.104156675 ADAM6; — intergenic — haematopoietic_ G, LINC00226 and_lymphoid A tissue chr14 1.06E+08 C T, 96 742 56 234 0.012183 0.128666954 ADAM6; — intergenic — haematopoietic_ G LINC00226 and_lymphoid tissue chr14 1.06E+08 C G, 107 787 41 165 0.031477 0.103707699 ADAM6; — intergenic — haematopoietic_ A, LINC00226 and_lymphoid T tissue chr14 1.06E+08 T G, 101 660 28 73 0.003794 0.117463541 ADAM6; — intergenic — haematopoietic_ C, LINC00226 and_lymphoid A tissue chr14 1.06E+08 T C, 59 434 43 111 0.000131 0.217898903 ADAM6; — intergenic — haematopoietic_ A, LINC00226 and_lymphoid G tissue chr14 1.06E+08 C T 109 835 44 162 0.004123 0.125094237 ADAM6; — intergenic — haematopoietic_ LINC00226 and_lymphoid tissue chr14 1.06E+08 C T, 45 315 52 185 0.028068 0.166082474 ADAM6; — intergenic — haematopoietic_ G, LINC00226 and lymphoid A tissue chr2 88857538 C T, 28 680 230 1863 2.73E−06 0.158873576 MIR4436A; — intergenic — haematopoietic_ G LOC107985911 and_lymphoid_ tissue; cervix; lung chr2 88857650 G A 17 771 241 1761 5.84E−13 0.238610897 MIR4436A; — intergenic — haematopoietic_ LOC107985911 _and_lymphoid tissue; cervix; lung chr2 88861908 T C, 123 1237 67 397 0.015042 0.109669523 MIR4436A; — intergenic — haematopoietic_ G, LOC107985911 and_lymphoid A tissue chr2 88947319 T C, 74 930 130 720 5.65E−06 0.200891266 MIR4436A; — intergenic — haematopoietic_ G LOC107985911 and_lymphoid tissue chr2 88947322 G A 53 633 153 1038 0.010237 0.121533527 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947331 A C, 110 1041 91 561 0.041735 0.102549052 MIR4436A; — intergenic — haematopoietic_ G, LOC107985911 and_lymphoid T tissue chr2 88947332 G A, 100 1039 103 620 0.004423 0.133670055 MIR4436A; — intergenic — haematopoietic_ C, LOC107985911 _and_lymphoid T tissue chr2 88947333 T C, 93 969 111 691 0.008419 0.127852587 MIR4436A; — intergenic — haematopoietic_ A LOC107985911 and_lymphoid tissue chr2 88947343 A T, 47 625 139 837 0.000219 0.174808408 MIR4436A; — intergenic — haematopoietic_ G LOC107985911 and_lymphoid tissue chr2 88947349 C T 107 1094 99 576 0.002974 0.135672345 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947351 G C 96 1023 110 642 0.001244 0.148394997 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947353 A G 125 1238 81 428 0.001076 0.136301122 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947397 G A, 86 882 95 590 0.018497 0.124046661 MIR4436A; — intergenic — haematopoietic_ T LOC107985911 and_lymphoid tissue chr2 88947404 C T, 95 964 87 510 0.008089 0.132024692 MIR4436A; — intergenic — haematopoietic_ G LOC107985911 and_lymphoid tissue chr2 88947493 G A, 107 1024 63 349 0.01724 0.116400326 MIR4436A; — intergenic — haematopoietic_ C, LOC107985911 and_lymphoid T tissue chr2 88947501 T A, 124 1151 51 252 0.007712 0.111813461 MIR4436A; — intergenic — haematopoietic_ C, LOC107985911 and_lymphoid G tissue chr2 88947504 T C 69 737 105 690 0.030668 0.119916391 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947558 T A 87 923 89 488 0.001056 0.159827814 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 88947562 A G, 74 788 79 476 0.012872 0.139757591 MIR4436A; — intergenic — haematopoietic_ T, LOC107985911 and_lymphoid C tissue chr2 89040248 A G 28 310 123 687 0.021248 0.125502335 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 89142767 C T, 131 1426 78 430 0.000287 0.141524707 MIR4436A; — intergenic — haematopoietic_ G, LOC107985911 and_lymphoid A tissue chr2 89142777 C T, 131 1409 80 461 0.001032 0.132622855 MIR4436A; — intergenic — haematopoietic_ A, LOC107985911 and_lymphoid G tissue chr2 89319775 C T, 83 862 72 447 0.028373 0.123034082 MIR4436A; — intergenic — haematopoietic_ A, LOC107985911 and_lymphoid G tissue chr2 89319782 G C 94 950 68 416 0.031692 0.115214287 MIR4436A; — intergenic — haematopoietic_ LOC107985911 and_lymphoid tissue chr2 89319825 T C, 114 1101 50 274 0.022449 0.105605322 MIR4436A; — intergenic — haematopoietic_ G, LOC107985911 and_lymphoid A tissue chr2 89319827 C T, 98 982 49 289 0.043838 0.105953318 MIR4436A; — intergenic — haematopoietic_ A, LOC107985911 and_lymphoid G tissue chr2 89319865 T C, 111 1057 42 216 0.020662 0.104831878 MIR4436A; — intergenic — haematopoietic_ G, LOC107985911 and_lymphoid A tissue chr22 22712918 G T, 42 529 44 238 0.004123 0.201328037 GGTLC2; — intergenic — large intestine A, MIR650 C chr22 22712933 C G 60 739 37 193 0.002672 0.174361754 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid tissue chr22 22712970 A G, 71 785 21 84 0.003833 0.131598039 GGTLC2; — intergenic — haematopoietic_ T, MIR650 and_lymphoid C tissue chr22 22712980 G C, 59 695 37 219 0.024431 0.14581054 GGTLC2; — intergenic — haematopoietic_ A MIR650 and_lymphoid tissue chr22 22712982 A G, 42 596 52 307 0.001375 0.213213638 GGTLC2; — intergenic — haematopoietic_ C MIR650 and_lymphoid tissue chr22 22712988 A C, 52 611 39 225 0.02053 0.159432673 GGTLC2; — intergenic — haematopoietic_ G MIR650 _and_lymphoid tissue chr22 22712997 A C, 47 577 46 302 0.041735 0.151051415 GGTLC2; — intergenic — haematopoietic_ G, MIR650 and_lymphoid T tissue chr22 22713005 G A, 59 697 26 145 0.032566 0.133673327 GGTLC2; — intergenic — haematopoietic_ C, MIR650 and_lymphoid T tissue chr22 22713022 A G, 67 781 30 161 0.014607 0.138365399 GGTLC2; — intergenic — haematopoietic_ T MIR650 and_lymphoid tissue chr22 22713038 T C. 59 727 35 200 0.011145 0.156590695 GGTLC2; — intergenic — haematopoietic_ G MIR650 and_lymphoid tissue chr22 22713044 G A 53 553 43 280 0.034847 0.147809486 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid tissue chr22 22713060 A G, 54 690 36 191 0.002731 0.183200908 GGTLC2; — intergenic — haematopoietic_ C, MIR650 and_lymphoid T tissue chr22 22713061 G A, 69 746 22 99 0.014946 0.124598478 GGTLC2; — intergenic — haematopoietic_ C, MIR650 and_lymphoid T tissue chr22 22713064 A T, 58 648 38 228 0.04829 0.135559361 GGTLC2; — intergenic — large intestine G, MIR650 C chr22 22713166 A T, 45 587 46 277 0.007861 0.184892654 GGTLC2; — intergenic — cervix G, MIR650 C chr22 22713180 G C 45 624 52 328 0.004234 0.191544659 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid tissue chr22 22895517 C T 72 798 125 859 0.023839 0.116111007 IGLL5 NM_001256296 c.C243T exonic synonymous_ ovary SNV chr22 28798829 T C 81 320 85 139 8.86E−05 0.209215949 XBP1 — intronic — haematopoietic_ and_lymphoid tissue; lung

TABLE 17 Expanded mutations in endothelial cells FDR diff_ALT_ REF_endothelial REF_other ALT_endothelial ALT_other adj cellular cell cell cell cell P- prevalence_ Gene RefSeq_ Func_ Mutation_ CHR POS REF ALT # # # # values endothelial Name ID Coding type type Cosmic chr10 1.7E+07 C T 28 497 113 1019 0.0380461 0.129254851 VIM — UTR3 — lung;large_ intestine chr11 6.6E+07 C T 43 934 129 1477 0.0110995 0.137391124 MALAT1; — ncRNA_ — lung TALAM1 exonic chr11 6.6E+07 C T 18 559 158 1847 0.0032383 0.1300631 MALAT1; — ncRNA_ — central_nervous_ TALAM1 exonic system chr15 4.5E+07 C T 68 1437 93 976 0.0011808 0.173163995 B2M — UTR3 — lung;thyroid; large_intestine chr16 1964501 C T 67 1261 61 524 0.0011666 0.183005077 RPS2 NM_002952 c.G125A exonic non- lung synonymous_ SNV chr17 6.4E+07 G A 70 914 82 588 0.012879 0.147995655 DDX5 — UTR3 — haematopoietic_ and_lymphoid_ tissue chr19 4.2E+07 C T 62 1090 66 591 0.007725 0.164048557 RPS19 NM_001321485 c.C268T exonic non- lung synonymous_ SNV chr4 8.8E+07 C T 31 65 111 103 0.0367043 0.168594903 SPARCL1 — UTR5 — pancreas;large_ intestine chr4 8.8E+07 T C 32 61 107 89 0.03605 0.176450839 SPARCL1 — UTR5 — soft tissue chr6 3E+07 G A 72 1163 58 400 0.0003172 0.19023574 HLA- NM_001242758 c.G203A exonic non- thyroid A synonymous_ SNV chr6 3E+07 G A 67 1129 65 438 4.04E−05 0.212909246 HLA- NM_001242758 c.G271A exonic non- thyroid A synonymous_ SNV chr6 3E+07 G C 68 1117 62 444 0.0004052 0.192490021 HLA- NM_001242758 c.G282C exonic non- thyroid A synonymous_ SNV chr6 3E+07 T C 70 1080 66 495 0.0025336 0.171008403 HLA- NM_001242758 c.T385C exonic non- thyroid;urinary_ A synonymous_ tract SNV chr6 3E+07 G A 64 1019 72 558 0.0025336 0.175575366 HLA- NM_001242758 c.G489A exonic synonymous_ thyroid A SNV chr6 3E+07 C T 71 1115 65 470 0.0009615 0.181411208 HLA- NM_001242758 c.C521T exonic non- upper_aerodigestive_ A synonymous_ tract;skin SNV chr6 3E+07 T C 72 1117 64 465 0.0013231 0.176656503 HLA- NM_001242758 c.T538C exonic synonymous_ oesophagus;large_ A SNV intestine; haematopoietic_and_ lymphoid_tissue chr6 3E+07 T G 66 999 70 586 0.0229369 0.144989794 HLA- NM_001242758 c.T539G exonic non- soft_tissue;pancreas; A synonymous_ large_intestine SNV chr6 3E+07 C T 71 1096 66 493 0.0022885 0.171493801 HLA- NM_ c.C545T exonic non- breast;upper_ A 001242758 synonymous_ aerodigestive_tract SNV chr6 3E+07 A C 65 1101 69 487 1.00E−04 0.20825031 HLA- NM_ c.A559C exonic non- thyroid;upper_ A 001242758 synonymous_ aerodigestive_tract SNV chr6 3E+07 G C 71 1212 63 366 4.75E−07 0.23821009 HLA- NM_ c.G570C exonic non- thyroid;large_ A 001242758 synonymous_ intestine;kidney;biliary_ SNV tract;haematopoietic_ and_lymphoid_ tissue;skin chr6 3E+07 T G, 68 1129 65 422 2.22E−05 0.216639277 HLA- NM_ c.T571C exonic non- upper_aerodigestive_ A, A 001242758 synonymous_ tract C SNV chr6 3E+07 C G 70 1439 78 567 2.25E−07 0.244374983 HLA- — UTR3 — upper_aerodigestive_ A tract;haematopoietic_ and_lymphoid_ tissue chr6 3E+07 C T 74 1468 78 596 2.02E−06 0.224398205 HLA- — UTR3 — upper_aerodigestive_ A tract;lung chr6 3.1E+07 T C 52 1149 94 560 9.50E−11 0.316158612 HLA- — UTR3 — upper_aerodigestive_ C tract chr6 3.1E+07 C G 57 1028 71 426 4.75E−07 0.261702631 HLA- NM_001243042 c.G270C exonic non- kidney; lung C synonymous_ SNV chr6 3.1E+07 C G 78 1391 74 525 6.69E−06 0.212833755 HLA- NM_005514 c.G540C exonic synonymous_ upper_aerodigestive_ B SNV tract;haematopoietic_ and_lymphoid_ tissue chr6 3.1E+07 G C 80 1391 72 505 1.06E−05 0.207334 HLA- NM_005514 c.C538G exonic non- upper_aerodigestive_ B synonymous_ tract SNV chr6 3.1E+07 C T 76 1356 75 563 3.00E−05 0.203306772 HLA- NM_005514 c.G412A exonic non- soft_tissue;urinary_ B synonymous_ tract; thyroid; biliary_ SNV tract chr6 3.1E+07 A C 73 1334 72 518 8.69E−06 0.2168541 HLA- NM_005514 c.T97G exonic non- upper_aerodigestive_ B synonymous_ tract;central_ SNV nervous_system chr6 3.2E+07 A T 22 424 31 120 2.64E−06 0.364317425 ATP6V1G2- — ncRNA_ — upper_aerodigestive_ DDX39B intronic tract;thyroid;skin; haematopoietic_ and lymphoid_tissue chr8 6562969 C G 27 86 113 109 0.0002761 0.248168498 ANGPT2 — UTR5 — haematopoietic_and_ lymphoid_tissue; lung chr8 6563013 A G 22 89 117 92 3.39E−07 0.333439326 ANGPT2 — UTR5 — haematopoietic_and_ lymphoid_tissue; lung chr8 6563053 A G 29 86 111 106 0.0005323 0.24077381 ANGPT2 — UTR5 — large_intestine chr9 1.4E+08 G A 0 27 166 106 8.00E−07 0.203007519 EGFL7 — UTR3 — liver chrM 10685 G A 44 751 63 525 0.0126112 0.17734304 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 14159 C T, 54 721 56 373 0.0150223 0.168140269 MIR12136; — intergenic — large_intestine; A NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 14846 G A 76 1180 74 603 0.0054693 0.155139278 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 15041 G A 74 1210 82 646 0.0007761 0.177580681 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 15437 G A 81 1346 81 663 0.0009352 0.169985067 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 1792 G A 49 1108 127 1463 0.0033645 0.152551625 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 1905 C T, 36 869 141 1698 0.0079399 0.135137633 NONE; — intergenic — large_intestine; G, MIR12136 breast;haematopoietic_ A and_lymphoid_tissue; stomach chrM 1948 C T 32 777 145 1801 0.0188776 0.120605471 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 3021 C T 64 1166 107 1187 0.0458633 0.121268606 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 3954 C T 63 1269 92 1023 0.012032 0.147213309 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 5980 C T 74 1380 84 742 0.0004052 0.181975447 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 6173 C T 44 1016 116 1142 0.0001818 0.195806302 NONE; — intergenic — large_intestine; MIR12136 breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 6957 G A 35 906 135 1459 0.0003725 0.177204328 MIR12136 — downstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 7215 C T 49 1030 125 1452 0.0160527 0.133378718 MIR12136 — downstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 7235 C T 44 1026 130 1459 0.0019012 0.1600037 MIR12136 — downstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 7792 C T 50 1194 124 1354 0.0003433 0.181246504 MIR12136 — upstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 8019 C T 23 598 151 1964 0.0456478 0.101227489 MIR12136 — upstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 8140 C T, 37 1054 137 1512 3.38E−05 0.198112362 MIR12136 — upstream — large_intestine; A breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 8372 C T 69 1290 88 853 0.0031903 0.162469423 MIR12136 — upstream — large_intestine; breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 8841 C T 61 1144 107 1200 0.0389821 0.124959369 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrM 9777 G A 54 1117 121 1419 0.0186474 0.131885985 MIR12136; — intergenic — large_intestine; NONE breast;haematopoietic_ and_lymphoid_tissue; stomach chrX 2.4E+07 G T 56 822 96 460 6.03E−08 0.272764595 SAT1 — UTR5 — haematopoietic_and_ lymphoid_tissue; lung chrX 2.4E+07 C T 31 324 71 282 0.0012703 0.230731897 SAT1 — intronic — lung

TABLE 18 Expanded mutations in follicular B cells REF_follicular ALT_follicular diff_ALT_cellular B REF_other B ALT_other prevalence_ cell cell cell cell FDR adj follicular Gene RefSeq_ CHR POS REF ALT numbers numbers numbers numbers P-values B vs Name ID Coding Func_type Mutation_type Cosmic chr11 65499858 A G 34 2024 150 632 1.98E−50 0.577265584 MALAT1; — ncRNA_ — lung TALAM1 exonic chr22 22720669 T C 19 313 95 110 1.06E−25 0.573286052 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid_ tissue chr22 22720862 G A, 12 289 109 140 9.51E−26 0.57448612 GGTLC2; — intergenic — haematopoietic_ C MIR650 and_lymphoid_ tissue chr22 22720863 G A, 11 275 111 147 9.71E−25 0.561494833 GGTLC2; — intergenic — haematopoietic_ C, MIR650 and_lymphoid_ T tissue chr22 22720950 A G, 14 276 106 151 7.64E−22 0.529703357 GGTLC2; — intergenic — haematopoietic_ C, MIR650 and_lymphoid_ T tissue chr22 22720978 A G 9 188 113 261 3.58E−10 0.344937749 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid_ tissue chr22 22721004 A C, 9 249 112 173 8.59E−21 0.515667228 GGTLC2; — intergenic — haematopoietic_ T, MIR650 and_lymphoid_ G tissue chr22 22721048 C G, 11 311 111 140 1.05E−28 0.59941478 GGTLC2; — intergenic — haematopoietic_ T MIR650 and_lymphoid_ tissue chr22 22721060 C G, 10 275 112 160 5.99E−24 0.550216695 GGTLC2; — intergenic — haematopoietic_ T MIR650 and_lymphoid_ tissue chr22 22721063 A G, 24 286 96 143 3.69E−17 0.466666667 GGTLC2; — intergenic — haematopoietic_ T MIR650 and_lymphoid_ tissue chr22 22721066 A G, 14 287 108 135 3.02E−25 0.565340688 GGTLC2; — intergenic — haematopoietic_ C MIR650 and_lymphoid_ tissue chr22 22721096 T C 14 298 109 152 2.93E−24 0.548401084 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid_ tissue chr22 22721123 C T 10 217 111 227 2.30E−13 0.406094111 GGTLC2; — intergenic — haematopoietic_ MIR650 and_lymphoid_ tissue chr22 22721127 A C, 12 248 112 150 9.94E−22 0.526341384 GGTLC2; — intergenic — haematopoietic_ G, MIR650 and_lymphoid_ T tissue chr22 22721129 C G, 8 234 113 171 2.25E−20 0.511662075 GGTLC2; — intergenic — haematopoietic_ T MIR650 and_lymphoid_ tissue chr22 22899624 A C, 32 721 126 731 4.83E−10 0.294024828 IGLL5; — intergenic — ovary T, RSPH14 G chr22 22906484 C T 58 923 108 698 8.19E−06 0.220004014 IGLL5; — intergenic — large_intestine RSPH14 chr6 31533435 A T 8 438 15 136 0.00089 0.415240115 ATP6V1G2- — ncRNA_ — upper_ DDX39B intronic aerodigestive_ tract; thyroid; skin; haematopoietic_ and_lymphoid tissue chr6 32444789 T A 27 450 48 266 0.00052 0.26849162 HLA-DRA — UTR3 — upper_ aerodigestive_ tract chr6 32444815 G A 27 420 51 303 0.00317 0.234759017 HLA-DRA — UTR3 — upper_ aerodigestive_ tract chr6 32445032 T A 31 452 34 199 0.0111 0.217393359 HLA-DRA — UTR3 — upper_ aerodigestive_ tract chr6 32578889 G A 46 753 48 357 0.00663 0.189016676 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32578907 A G 45 760 47 350 0.00483 0.19555425 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32578920 G A 43 710 51 388 0.00804 0.189183428 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32578921 G A 43 743 51 366 0.00169 0.21252614 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32579023 A G 47 740 43 324 0.01684 0.1732665 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32579069 C T 37 664 52 393 0.0032 0.212462662 HLA-DRB1 — UTR3 — haematopoietic_ and_ lymphoid_tissue; liver;large_ intestine chr6 32584162 G T, 38 665 36 300 0.03585 0.175605657 HLA-DRB1 NM_002124 c.C317G exonic non- haematopoietic_ C synonymous_ and_ SNV lymphoid_tissue; liver;large_ intestine chr6 32589702 G A 44 699 32 241 0.03585 0.164669653 HLA-DRB1 NM_002124 c.C41T exonic non- haematopoietic_ synonymous_ and_ SNV lymphoid_tissue; liver;large_ intestine

TABLE 19 Expanded mutations in T & NK cells diff_ALT_ cellular ALT FDR prevalence_ REF_T&NK REF_other ALT_T&NK other adj T&NK cell cell cell cell P- vs Gene RefSeq_ Func_ Mutation_ CHR POS REF ALT numbers numbers numbers numbers values others Name ID Coding type type Cosmic chr14 1.06E+08 C G, 24 245 43 157 0.001602 0.251243781 ADAM6; — intergenic — ovary A, LINC00226 T chr14 1.06E+08 G A 35 275 39 120 0.002481 0.223229559 ADAM6; — intergenic — ovary C LINC00226 chr14 1.06E+08 G A 37 279 37 131 0.023736 0.180487805 ADAM6; — intergenic — ovary LINC00226 chr17 6774861 A C 17 168 30 118 0.034784 0.22571046 XAF1 — UTR3 — stomach chr6 29942886 G A 146 1089 119 339 1.01E−10 0.211661646 HLA-A NM_001242758 c.G203A exonic non- thyroid synonymous_ SNV chr6 29942954 G A 126 1070 135 368 5.54E−15 0.261330392 HLA-A NM_001242758 c.G271A exonic non- thyroid synonymous_ SNV chr6 29942965 G C 128 1057 134 372 8.50E−14 0.251128478 HLA-A NM_001242758 c.G282C exonic non- thyroid synonymous_ SNV chr6 29943309 T C 126 1024 137 424 4.32E−11 0.228094868 HLA-A NM_001242758 c.T385C exonic non- thyroid;urinary_tract synonymous_ SNV chr6 29943413 G A 124 959 145 485 1.26E−08 0.203160881 HLA-A NM_001242758 c.G489A exonic synonymous_ thyroid SNV chr6 29943445 C T 131 1055 140 395 3.47E−13 0.244191373 HLA-A NM_001242758 c.C521T exonic non- upper_aerodigestive_ synonymous_ tract;skin SNV chr6 29943462 T C 136 1053 131 398 1.67E−10 0.216343113 HLA-A NM_001242758 c.T538C exonic synonymous_ oesophagus;large_ SNV intestine; haematopoietic_and_ lymphoid_tissue chr6 29943463 T G 124 941 145 511 2.53E−07 0.187105083 HLA-A NM_001242758 c.T539G exonic non- soft_tissue;pancreas;large_ synonymous_ intestine SNV chr6 29943469 C T 127 1040 144 415 3.81E−13 0.246141946 HLA-A NM_001242758 c.C545T exonic non- breast;upper_ synonymous_ aerodigestive_tract SNV chr6 29943483 A C 135 1031 130 426 1.26E−08 0.198184432 HLA-A NM_001242758 c.A559C exonic non- thyroid;upper_ synonymous_ aerodigestive_tract SNV chr6 29943494 G C 148 1135 113 316 1.84E−11 0.215169351 HLA-A NM_001242758 c.G570C exonic non- thyroid;large_intestine; synonymous_ kidney;biliary_tract; SNV haematopoietic_and_ lymphoid_tissue;skin chr6 29943495 T G, 138 1059 119 368 1.83E−09 0.205151347 HLA-A NM_001242758 c.T571C exonic non- upper_aerodigestive_tract A, synonymous_ C SNV chr6 29945597 C G 150 1359 169 476 1.51E−19 0.270380019 HLA-A — UTR3 — upper_aerodigestive_ tract; haematopoietic_and_ lymphoid_tissue chr6 29945653 C T 157 1385 174 500 1.23E−18 0.260427769 HLA-A — UTR3 — upper_aerodigestive_ tract;lung chr6 31269077 T C 114 1087 180 474 3.70E−21 0.308593393 HLA-C — UTR3 — upper_aerodigestive_tract chr6 31271672 C G 121 964 145 352 2.49E−16 0.277635578 HLA-C NM_001243042 c.G270C exonic non- kidney;lung synonymous_ SNV chr6 31356246 C G 177 1292 153 446 3.95E−12 0.207019563 HLA-B NM_005514 c.G540C exonic synonymous_ upper_aerodigestive_ SNV tract; haematopoietic_and_ lymphoid_tissue chr6 31356248 G C 179 1292 151 426 1.31E−12 0.20961301 HLA-B NM_005514 c.C538G exonic non- upper_aerodigestive_tract synonymous_ SNV chr6 31356374 C T 172 1260 161 477 4.81E−12 0.208872085 HLA-B NM_005514 c.G412A exonic non- soft_tissue;urinary_ synonymous_ tract;thyroid; SNV biliary_tract chr6 31356934 A C 173 1234 154 436 3.90E−12 0.209870168 HLA-B NM_005514 c.T97G exonic non- upper_aerodigestive_ synonymous_ tract;central SNV nervous_system chr6 31533435 A T 27 419 31 120 7.24E−06 0.31184825 ATP6V1G2- — ncRNA_ — upper_aerodigestive_ DDX39B intronic tract;thyroid;skin; haematopoietic_ and_lymphoid tissue chr6 32578849 C G, 4 110 62 439 0.0495 0.139758238 HLA- — UTR3 — haematopoietic_and_ A, DRB1 lymphoid_tissue;liver; T large_intestine

TABLE 20 Expanded mutations in plasma B cells diff_ALT_ REF_ REF_ ALT_ ALT_ cellular plasma other plasma other FDR prevalence_ B cell cell B cell cell adj plasma B num- num- num- num- P- vs Gene RefSeq_ Func_ Mutation_ CHR POS REF ALT bers bers bers bers values others Name ID Coding type type Cosmic chrX 4.8E+ T C 14 446 29 126 1.97E− 0.454138884 TIMP1 NM_ c.T372C exonic synonymous_ lung 07 07 003254 SNV

The foregoing description of illustrative embodiments of the invention has been presented for purposes of illustration and of description. It is not intended to be exhaustive or to limit the invention to the precise form disclosed, and modifications and variations are possible in light of the above teachings or may be acquired from practice of the invention. The embodiments were chosen and described in order to explain the principles of the invention and as practical applications of the invention to enable one skilled in the art to utilize the invention in various embodiments and with various modifications as suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims appended hereto and their equivalents.